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Biphasic activation of two mitogen-activated protein kinases during the cell cycle in mammalian cells

, : Biphasic activation of two mitogen-activated protein kinases during the cell cycle in mammalian cells. Journal of Biological Chemistry 267(28): 20293-7

We studied mitogen-activated protein kinase (MAPK) activities during the cell cycle of Chinese hamster ovary (CHO) cells using site-specific antibodies against extracellular signal-regulated kinase-1, a 44-kDa MAPK (Boulton, T.G., Yancopoulos, G.D., Gregory, J.S., Slauer, C., Moomaw, C., Hsu, J., and Cobb, M.H. (1990) Science 249, 64-67). These antibodies detected two distinct MAPKs (44- and 42-kDa MAPKs) in CHO cells. CHO cells were arrested at metaphase in the M phase by treatment with nocodazole, and activities of MAPKs were analyzed at specific time points after release from arrest. Immune complex kinase assay and renaturation and phosphorylation assay in substrate-containing gel revealed that both 44- and 42-kDa MAPKs had activities in the G1 through S and G2/M phases and were activated biphasically, in the G1 phase and around the M phase. MAPKs were inactivated in metaphase-arrested cells. The amount of MAPKs did not change significantly in the cell cycle. In the G1, S, and G2/M phases, MAPKs were phosphorylated on both tyrosine and threonine residues and dephosphorylated in metaphase-arrested cells. Our data suggest that MAPKs may play some role in the cell cycle other than G0/G1 transition.


PMID: 1400347

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Eliades, G.; Georgiou, A.; Papadopoulos, I., 2004: Effect of conventional irrigation and alternate rootzone drying with sufficient or deficient irrigation water on the production of Marsh seedless grapefruit. The partial root zone drying (PRD) technique by which water is delivered alternately on either side of the tree, and conventional irrigation (CI) where water is applied on both sides, combined with sufficient or deficient water, were tested with M...

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Mitchell G.P.; Mutch Alexander D., 1956: Geology of the Hardy Mine, Sudbury District, Ontario. Canadian Mining and Metallurgical Bulletin (Canadian Institute of Mining and Metallurgy) Montreal

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Smolinskaya V.A.; Mazepa I.V., 1972: Copper zinc barium and manganese metabolism in blood diseases. Mikroelementy v Meditsine Respublikanskii Mezhvedomstvennyi Sbornik 3: 75-77