+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

CAT vectors for analysis of eukaryotic promoters and enhancers

, : CAT vectors for analysis of eukaryotic promoters and enhancers. Gene 45(1): 107-111

We have constructed two sets of plasmids for analysis of factors affecting mammalian gene expression. The pOCAT series contains a bacterial chloramphenicol-resistance expression unit (cat) and no eukaryotic promoter. The pUTKAT series contains the same cat unit under the control of the thymidine-kinase promoter of Herpes simplex virus. These plasmids are designed for testing effects of inserted regulatory elements on cat expression after transient transfection of mammalian cells in culture. We demonstrate here that the pOCAT series is useful for studying activities of inserted eukaryotic promoters, and the pUTKAT series is useful for studying activities of inserted eukaryotic enhancers.


PMID: 3023200

Other references

Morishita, K., 1931: Dengue Fever, especially recent Studies on it.. In the course of this paper it is stated that Aedes argenteus, Poir. (aegypti, auct.) is generally found on the plains near the coast in Formosa and is common in the Pescadores Islands. A. albopictus, Skuse, is also common in the Islands.

Brosious-Henson, E.; Abbey, M.J.; Fritz, P.D.; Guzik, A., 1996: NP of the year: profiles in practice innovation. Np News 4(3): 1, 4-1, 4

Boeger, W.A.; Husak, W.S.; Martins, M.L., 1995: Neotropical Monogenoidea 25 Anacanthorus penilabiatus n sp Dactylogyridae, Anacanthorinae from Piaractus mesopotamicus Osteichthyes, Serrasalmidae, cultivated in the State of Sao Paulo,; Brazil. Anacanthorus penilabiatus n. sp. is described from the serrasalmid fish, Piaractus mesopotamicus (Holmberg, 1887), cultivated in the Centro de Aqueicultura, Universidade Estadual Paulista. The new species is characterized by having a relatively st...

Zambrana-García, J.L.; Macías Blanco, C.; Fernández-Suárez, A.; Peñacoba Masa, A.; Olivares Durán, M.J.; Aguilar Benítez, J.M.; Zambrana-Luque, J.L., 2017: Impact of a do-not-do intervention on 12 laboratory measurements. In recent years, various scientific societies and healthcare organisations have created recommendations aimed at decreasing the use of healthcare interventions that have shown no efficacy or effectiveness. The aim of this study was to assess the i...

Mitchell, D.; Beatty, E.T.; Cox, P.K., 1977: Behavioral differences between 2 populations of wild rats implications for domestication research. Wild rats [Rattus norvegicus] were habituated to a familiar food prior to being offered a choice between a familiar and a novel food. There was a significant difference between the 2 strains in their neophobic avoidance of the novel food. Animals...

Whitacre, J.; Yarnell, S.H.; Oberg, A.G.; Mccrery, J.; Mcwhirter, L., 1944: Calcium, phosphorus, and iron contents of turnip greens as influenced by method of sampling. Fe estimations were made by a procedure based 011 the o-phenanthroline method of Saywell and Cunningham (Abst. 58, Vol. 7). For moisture, Ca and P. methods of the A. O. A. C. 1940 were used. Three methods of sampling were employed. One consisted i...

Khan, M.A.; Alahyan, M.; Gaylor, V.; Lam, K.; Yacoub, M., 1998: Cytomegalovirus induced activation of MAP kinase in human coronary artery smooth muscle cells. FASEB Journal 12(8): A1308, April 24

Coutinho, Elsimar-Metzker, 1993: One year contraception with a single subdermal implant containing nomegestrol acetate (Uniplant). One single silastic capsule containing nomegestrol acetate, Uniplant, was inserted subcutaneously in 100 women of reproductive age who desired to avoid conception. Insertions and removals of the capsules were made in the gluteal region following i...

Ricci, M.; Aliquo, M.R.; Bottinelli, G.; Mascolo, A.; Piro, R., 1989: Enrichment in Rappaport Vassiliadis Broth in the isolation of Salmonella in foods in relation to the official method. Rappaport Vassiliadis Broth [Rappaport et al. Journal of Clinical Pathology (1956) 9, 261] showed a greater sensitivity and specificity as an enrichment medium in the isolation of Salmonella in food than the traditional medium selenite broth. Usin...

Gill, D.; Cobcroft, R.G.; Seldon, M.; Nottage, M.; Enno, A.; Deveridge, S.F.; Marlton, P.V.; Spencer, A.S., 1998: Oral therapy followed by autologous PBSCT in de novo multiple myeloma. Blood 92(10 SUPPL 1 PART 1-2): 364B, Nov 15