geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

CHAPS solubilization of a G-protein sensitive 5-HT1A receptor from bovine hippocampus


, : CHAPS solubilization of a G-protein sensitive 5-HT1A receptor from bovine hippocampus. Life Sciences 45(21): 1997-2005

The binding of [3H] 8-OH-DPAT to membrane-bound 5-HT1A receptors from bovine hippocampus was saturable and corresponded to a single high-affinity state. Solubilization of the bovine hippocampal membranes with 10 mM CHAPS containing 200 mM NaCl, renders a preparation which binds [3H] 8-OH-DPAT with high-affinity (Kd = 1.9 nM) and is guanine nucleotide sensitive and ketanserin insensitive. 50% of [3H] 8-OH-DPAT binding activity is solubilized. The presence of GMP-P(NH)P promotes a low-affinity (Kd = 9.6 nM) state which is characteristic of receptors coupled to G-proteins. GMP-P(NH)P markedly accelerates the dissociation [3H] 8-OH-DPAT from solubilized membranes while having negligible effects on association. Thus, the agonist can activate the terniary complex rather than to promote its formation. 8-OH DPAT, WB 4101 and 5-carboxamidotryptamine dose responsively inhibit soluble [3H] 8-OH-DPAT binding with IC(50) values of 16.1, 15.6 and 1.3 nM, respectively. The CHAPS solubilized membrane preparation retains many of the [3H] 8-OH-DPAT binding characteristics of the membrane bound form.

US$29.90

PMID: 2532283


Other references

Shevchuk, T.V.; Zakharchenko, N.S.; Dyachenko, O.V.; Buryanov, Y.I., 2001: The effect of the gene encoding EcoRII DNA methyltransferase on the phenotype of transgenic tumor cell lines of Nicotiana tabacum and the methylation level of CpNpG sequences in their genome. Several tumor cell lines were obtained by transforming Nicotiana tabacum plants with the recombinant Ti plasmid comprising the gene encoding EcoRII DNA methyltransferase (M.EcoRII) and subjected to analysis. The transformed lines differed in their...

Sinha, K.P.; Goldberg, H., 1958: Marfan's syndrome: a case with complete dissection of the aorta. American Heart Journal 56(6): 890-897

Herrmann, M., 2014: Antimicrobial effects promoting biofilm formation and persistent disease: the role of a DNA-binding regulator, SarA, in staphylococcal endocarditis. Journal of Infectious Diseases 209(8): 1153-1155

Shen Qizhang., 1990: In low temperature keep the embryo of Protosalanx hyalocranius. Zoological Research, 113: 258

Tryon, G.W., 1880: Manual of Conchology, vol. ii. Muricinae, Purpurinae. G. W. TRYON gives in the second volume of his Manual of Conchology, pp. 1-71, a general sketch of the external features, shell, anatomy, distribution in time and space, and history of classification of the Prosobranchiate, making free use of Kiefe...

Buggle, K.; Delahunty, J.J.; Philbin, E.M., 1971: N.M.R. and I.R. spectra of some -arylthiocinnamic acids and esters. Proceedings of the Royal Irish Academy. Section B: Biological, Geological, and Chemical Science 71(17): 257-267

Hayflick, L., 1974: Biomedical gerontology. Current theories of biological aging. Gerontologist 14(5 Pt 1): 454, 458-454, 458

Giedosz, B., 1938: The effect of vitamin E on the histological picture of the endocrine glands. DAILY injection of 2 c.c. of a solution of vitamin E in oil for 5 days failed to induce histological changes in the gonads and other endocrine glands of and guinea-pigs.

Nelson, M Lee; Mcmahon, T.E.; Thurow, R.F., 2002: Decline of the migratory form in bull charr, Salvelinus confluentus, and implications for conservation. Large-bodied, migratory life history forms of bull charr, Salvelinus confluentus, were historically abundant in northwestern North America, but many remaining populations of this now-threatened species presently persist as small-bodied residents i...

Li-Zhen-Ting; Ma-Yao-Feng, 2007: Comparison research on demographic character of inbound tourists. Based on the data of investigation, the demographic characters of inbound tourists are researched. It is found that there are some common demographic characters of inbound tourists and different cities of our six big tourism cities have its own c...