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Characterisation and specificity of glomerular basement membrane antigens identified by sera of patients with anti-GBM nephritis

, : Characterisation and specificity of glomerular basement membrane antigens identified by sera of patients with anti-GBM nephritis. Nephrology, Dialysis, Transplantation 1(3): 155-163

The sera of 21 patients positive for antibodies against GBM in indirect immunofluorescence tests were examined by immunoblotting. We demonstrated antibodies against 50, 48, 43 and 29 kD molecular weight peptides in 20 of 21 sera using collagenase-digested GBM, in 19 of 21 using trypsin-digested GBM, and in 10 of 21 using elastase-digested GBM. Although the spectrum of molecular weights of the antigenic proteins was similar in all three digests, they differed with respect to preservation of antigenicity upon reduction with mercaptoethanol. Many of the sera of patients and controls reacted with proteins unrelated to GBM, e.g. albumin and prealbumin. Furthermore, some control sera reacted with one single peptide of the above-mentioned specific GBM peptides. Our results suggest that the highly purified 29 kD peptide of the collagenase digest or the 50 kD peptide of the trypsin digest provide the best antigens to develop a screening test for antibodies against GBM. However, serum antibodies against these antigens will not be absolutely specific for anti-GBM antibody-mediated nephritis, as shown by the immunoblot experiments.


PMID: 3110669

Other references

Reed, A., 1969: Marriage guidance: the counsellor and the client. District Nursing 12(6): 104-105

Kirley, B.B.; Farnworth, B.; Zhang, C.; Worley, C.K.; Lynch, J.Z.; Sakai, H.; Shapiro, A.D., 2003: Characterization of Arabidopsis mutants with alterations in disease resistance-associated PCD. Activation tagged populations of Arabidopsis, carrying a strong enhancer element randomly dispersed throughout the genome such that individual plants overexpress one or a few genes, were screened for alterations in a programmed cell death (PCD) re...

Bisaillon A.; Pierard J.; Lariviere N., 1976: The cervical segment of carnivores adapted to aquatic life mammalia carnivora. Canadian Journal of Zoology 54(4): 431-436

Scriver, C.R., 1986: Cystinuria. Aetiology, diagnosis and treatment of cystinuria are discussed with reference to the findings of Jaeger et al. on anticystinuric effects of glutamine and of dietary sodium restriction.

Caglar, G.S.; Ozdemir, E.D.; Kiseli, M.; Demirtas, S.; Cengiz, S.D., 2015: The association of osteocalcin and adiponectin with glucose metabolism in nondiabetic postmenopausal women. This study was designed to determine if osteocalcin is associated with insulin resistance, metabolic risk factors and adiponectin levels in nondiabetic postmenopausal women. A total of 87 menopausal nondiabetic subjects were enrolled into the stud...

Mayston, M.J.; Harrison, L.M.; Stephens, J.A., 1997: Effects of transcranial magnetic brain stimulation in patients with callosal agenesis. Society for Neuroscience Abstracts 23(1-2): 2371

Zhang, Z.; Zhou, H.; Nightingale, C.-Louise., 1997: Phytal meiofauna of a rocky shore at the Cape d'Aguilar Marine Reserve, Hong Kong. Unknown

Mazursky, M.M.; Sawan, E., 1950: Female pseudohermaphroditism due to congenita adrenal cortical hyperplasia with associated Addisonian-like symptoms. Journal of Pediatrics 36(6): 789-796

Junk, Jürgen.; Helbig, A.; Krein, A., 2004: Screening and scenarios of traffic emissions at Trier, Germany. Scope and Background. In the course of the European Council Directive on permissible air pollutant limit values, valid starting from 2005 there is an urgent call for action, particularly for fine dust (PM10). Current investigations (Junk & Helbig...

Grabovskaya K.B., 1968: Comparative data on the distribution of colicins among different types of intestinal bacteria under conditions of intra species and inter species detection of them. Byulleten' Eksperimental'noi Biologii i Meditsiny 65(2): 64-67