+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization of specific binding sites for PAF in the iris and ciliary body of rabbit

, : Characterization of specific binding sites for PAF in the iris and ciliary body of rabbit. Biochemical and Biophysical Research Communications 160(1): 250-256

The protective effect exerted by BN 52021 a specific PAF-receptor antagonist in experimentally induced ocular inflammatory disorders led us to investigate the possible presence of specific receptors for PAF in rabbit iris and ciliary body. Two classes of PAF binding sites were found in isolated iris and ciliary process of pigmented rabbit eyes: a high affinity site Kd1 congruent to 4.9 +/- 0.47 nM, Bmax1 congruent to 3.17 +/- 0.50 pmoles/mg protein, a low affinity sites Kd2 congruent to 11.6 +/- 0.33 nM, Bmax2 congruent to 12.46 +/- 2.3 pmoles/mg protein for iris. The specific binding was not affected by lyso-PAF the biologically inactive precursor and metabolite of PAF, up to 10(-6) M; inhibition by unlabelled PAF demonstrated a biphasic curve partially antagonized by BN 52021. The present results demonstrate the presence of specific binding sites for PAF in rabbit eyes which could mediate the action of this mediator in eye inflammatory processes and explain the protective effect observed with BN 52021.


PMID: 2540746

Other references

Bi, P.; Zong, W.; Li, J.; Song, Y., 2002: The design of a novel multifunction ECG board. In this paper an ECG amplifying circuit is comprehensively presented. This circuit has all the necessary functions which include isolated ground, programmable ECG lead and filter band, programmed gain, rapid baseline restoring, anti defibrillating...

Chung, M.Woo.; Jeong, O.; Park, Y.Kyu.; Lee, K.Hwa.; Lee, J.Hyuk.; Lee, W.Sik.; Joo, Y.Eun.; Choi, S.Kyu.; Cho, S.Bum., 2014: Comparison on the long term outcome between endoscopic submucosal dissection and surgical treatment for undifferentiated early gastric cancer. There are controversies on the efficacy and safety of endoscopic submucosal dissection (ESD) for un-differentiated early gastric cancer (EGC) despite the expansion of ESD indications. The aim of this study was to evaluate the long term outcome of...

Awiti L.R.S., 1981: A strange multinuclear condition in the epithelial cells of the mesadenial accessory reproductive gland of dysdercus fasciatus. The structure and function of the male accessory reproductive gland system of the cotton stainer, D. fasciatus Signoret, were studied using ultrastructural techniques. The gland system is composed of a pair of mesadenia and an ejaculatory duct, wh...

Antelava, N.V., 1974: Nikolaĭ Vardenovich Antelava. Vestnik Khirurgii Imeni I. I. Grekova 113(8): 2-2

Dobbin Júnior, J.E.; Valença Júnior, J.V.; Cruz, A.E., 1969: Various reports on pulicideos from the floor of dwellings and wild animals in the Northeast of Brazil. Revista Brasileira de Malariologia E Doencas Tropicais. Publicacoes Avulsas 21(4): 735-743

Guenther, W.H.; Mautner, H.G., 1964: Analogs Of Parasympathetic Neuroeffectors. I. Acetylselenocholine, Selenocholine, And Related Compounds. Acetyl-selenocholine, benzoylselenocholine, butyrylselenocholine, selenocholine diselenide, and some of their dimethyl and monomethyl analogs were pre -pared. The use of the Bunte salts of selenocysteamines for the preparation of diselenides is de...

Bockaert J.; Delaage M., 1989: Inserm national institute of health and medical research colloquium vol 182 interactions between biology and chemistry molecular bases and applications 3 intracellular transmission of external signals summer school in pharmacochemistry 1986. Bockaert, J And M Delaage Colloque Inserm (Institut National De La Sante Et De La Recherche Medicale), Vol 182 Interactions Chimie-Biologie: Bases Moleculaires Et Applications, 3 Relais Intracellulaires Des Signaux Externes; (Inserm (National Institute Of Health And Medical Research) Colloquium, Vol 182 Interactions Between Biology And Chemistry: Molecular Bases And Applications, 3 Intracellular Transmission Of External Signals); Summer School in Pharmacochemistry, 1986 Xiv+302p

Boctor Nabil Z.; Frantz J.D., 1980: Mineral-solution equilibria in the system Mn (sub 3) O (sub 4) -H (sub 2) -H (sub 2) O-HCl. Year Book - Carnegie Institution of Washington 79): 345-347

Garrett, B.L., 2011: Videographic geographies Using digital video for geographic research. This article is a review of the ways in which human geography has engaged with film and video. Beginning with a look at the history of cinematic analysis within the discipline, the paper outlines different possible uses for digital video, focusing...

Berberovic L.J., 1967: Inst x ray investigations of morpho physiology of the intestinal tract in several birds columba livia buteo buteo and nycticorax nycticrax. Bulletin Scientifique Conseil des Academies de la RSF de Yougoslavie 12(11/12): 309-312