geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization of the inhibitory effect of lysolipids on perforin-mediated hemolysis


, : Characterization of the inhibitory effect of lysolipids on perforin-mediated hemolysis. Molecular Immunology 27(3): 257-261

The ability of lysolipids to inhibit the lytic activity of perforin from cytotoxic T lymphocytes was investigated. Sublytic concentrations of various lysolipids were incorporated into the membranes of sheep red blood cells (RBC) and the cells were then lysed with purified perforin. Lysophosphatidylcholines (lysoPC) can effectively block perforin-mediated lysis at micromolar concentrations. This is in marked contrast to phosphorylcholine, the putative calcium-dependent receptor for perforin, which inhibits lysis only at greater than or equal to millimolar concentrations. Unlike the inhibitory action of lipids, the lysolipids do not show a strict dependence on headgroup composition, as lysophosphatidylserine (lysoPS) is just as effective as lysoPC. All the lysoPC tested, ranging from lysolauroyl PC to lysostearoyl PC, are good inhibitors, with lysomyristoyl PC being the most effective. Binding of lysoPC to RBC is reversible; the inhibition by lysoPC can be removed with bovine serum albumin (BSA), and washing RBC that had been pretreated with lysoPC leads to a loss of inhibition. Binding of perforin to membranes is temperature-independent and precedes a temperature-dependent, insertion/pore-formation stage; hemolysis experiments that take advantage of this fact indicate that lysoPC acts mostly by blocking perforin binding to the RBC membranes.

US$29.90

PMID: 2342489


Other references

Tuigunov, M.M.; Gashimova, D.T.; Akhtareeva, A.A.; Gabidullin, Y.Z.; Bulgakov, A.K.; Davletshina, G.K.; Idiatullina, G.A., 2016: Morpho-Functional Characteristic Of Enterobacteria Adhesive Activity. Study adhesive activity of enterobacteria in the model of hemagglutination reaction with animal and avian erythrocytes and clarify structures responsible for adhesion in enterobacteria. 58 cultures of enterobacteria were used, of which: 21 Escheri...

Banerjee, D.; Liefshitz, A., 2001: Potential of the proteasomal inhibitor MG-312 as an anticancer agent, alone and in combination. Proteasomal activity is required for normal cellular functions including cell division, where entry and exit from mitosis is strictly regulated by cyclins and cyclin-dependent kinases which are among the important substrates of the proteasomal deg...

Swanson, G.J., 1988: Regeneration of sensory hair cells in the vertebrate inner ear. Trends in Neurosciences 11(8): 339-342

Koizumi, N.; Matsumiya, Y., 1997: Assessment of stream fish habitat based on Index of Biotic Integrity. We have assessed the stream fish habitat in the Kanto district, Japan based on IBI, Index of Biotic Integrity. The IBI, proposed by KARR (1981) is composed of 12 metrics to assess the attributes of fish communities, and indicates the sum of scores...

Sel Tser V.K., 1967: Physiological studies in radiation hygiene human. Trudy Leningradskogo Nauchno-Issledovatel'skogo Instituta Radiatsii i Gigieny (3): 112-121

Chemir, F.; Alemseged, F.; Workneh, D., 2014: Satisfaction with focused antenatal care service and associated factors among pregnant women attending focused antenatal care at health centers in Jimma town, Jimma zone, South West Ethiopia; a facility based cross-sectional study triangulated with qualitative study. Client satisfaction is essential for further improvement of quality of focused antenatal care and to provide uniform health care services for pregnant women. However, studies on level of client satisfaction with focused antenatal care and associat...

Pokrovski, A.A.; Kon', I.Y.; Natanson, A.O.; Kravchenko, L.V., 1971: Activity of some enzymes in liver and kidneys of rats in excess of vitamin D-2 and experimental rickets. Growing Wistar rats of initial weight 60 to 70 g, on a stock diet were given daily by stomach tube crystalline ergocalciferol in sunflower oil at the rate of 800 000 IU/kg bodyweight; control rats were given the oil alone. The rats were killed aft...

Woodside, J.S.; Peterson, E.W.; Dogwiler, T., 2008: Examination of the relationship between longitudinal profile and sediment mobility within a fluviokarst stream system. The complex drainage systems within karst settings can result in atypical longitudinal profiles. Features, such as cave entrances, can be expressed as anomalous "bumps" in the longitudinal profile of a stream if down-cutting has continue...

Roy S.; Tandon H.D., 1972: Pathology of acute rheumatic fever. Annals of Indian Academy of Medical Sciences 8(1): 99-102

Priĭma, G.Ia., 1978: Grigorii Iakovlevich Priima. On his 70th birthday. Fiziologicheskii Zhurnal SSSR Imeni I. M. Sechenova 64(6): 889-889