geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Chemically induced mutagenesis in a shuttle vector with a low-background mutant frequency


, : Chemically induced mutagenesis in a shuttle vector with a low-background mutant frequency. Proceedings of the National Academy of Sciences of the United States of America 83(10): 3402-3406

We have developed a recombinant DNA shuttle vector that permits the molecular analysis of mutations induced in human cells by chemical or physical mutagens. The vector is able to replicate as a plasmid in Escherichia coli and in Epstein-Barr virus (EBV)-transformed human lymphoblastoid cell lines and contains the herpes simplex virus type 1 thymidine kinase gene (HSV tk) as the target for mutagenesis studies. After introduction of the vector into an EBV-transformed lymphoblastoid cell line (LCL-721) by electroporation, approximately equal to 2% of the transfected cells expressed the vector-encoded gene for hygromycin resistance. Plasmid DNA isolated from cells immediately after selection for hygromycin resistance (10 population doublings posttransfection) contained mutations in the HSV tk gene at a frequency of 6 X 10(-5). Treatment of plasmid-bearing LCL-721 cells with N-ethyl-N-nitrosourea resulted in a dose-dependent increase of up to 15-fold in the frequency of mutations in the HSV tk gene. The dose-response for the induction of mutations in the plasmid-encoded gene closely paralleled that for the induction of mutations in the cellular gene for hypoxanthine (guanine) phosphoribosyltransferase.

US$29.90

PMID: 3010297


Other references

Meyerholz, G.; Gaskin, J., 1978: Environmental sanitation and management in disease prevention. Pork industry handbook Purdue University Cooperative Extension Service

Tiou, M.M.; Huang, S.C.; Huang, S.C.; Shieh, C.S.; Lee, C.H., 1995: Idiopathic common bile duct perforation: a case report. Spontaneous or idiopathic perforation of the bile duct (IPBD) in neonates and infants is a rare disease. An 1-year-5-month-old female child admitted with progressive abdominal distension and generalized jaundice. She had previously been treated fo...

Feckley David L., 1993: Hydrogeologic assessment of shallow flow systems in the Walnut Formation, central Texas. Abstracts with Programs - Geological Society of America 25(1): 10

Rosemary GIBSON.P.ilippa GANDER.D.wn ELDER, 2012: Factors differentiating infants identified by parents as problem sleepers, and those that are not. Sleep problems are common during infancy. Possible factors which contribute to parental perception of problem sleep were investigated as part of a pilot study collecting objective data on the sleep of 1-year-old New Zealand infants (n= 52 families...

Gist, D., H.; Dawes, S., M.; Turner, T., W.; Sheldon, S.; Congdon, J.D., 2002: Sperm storage in turtles: A male perspective. The storage and behavior of sperm collected from the epididymis of two emyiid turtles were examined. In Chrysemys picta, the weight of the epididymis does not change significantly throughout the year as does the testis. However, in this species, a...

Godson, G.N.; Roberts, R.J., 1976: dna, single stranded/*metab. Virology 73(2): 561-567

Hammel H.B.; Goguen J.D.; Sinton W.M.; Cruikshank D.P., 1983: Observational test for sulfur allotropes on Io. Bulletin of the American Astronomical Society 15(3): 850

Hartikka P.; Dahlbom M.; Westermarck H., 1983: Effect of iron saccharate injections of finnish horses. In 4 training stables 5 trotter horses of the Finnish cold-blooded breed and 7 warmblooded trotters were treated with a ferrisaccharate preparation (Hippiron) i.v. A short increase in heart frequency after the injection was noted. The cold solutio...

Lobban, F.; Robinson, H.; Appelbe, D.; Barraclough, J.; Bedson, E.; Collinge, L.; Dodd, S.; Flowers, S.; Honary, M.; Johnson, S.; Mateus, C.; Mezes, B.; Minns, V.; Murray, E.; Walker, A.; Williamson, P.; Wintermeyer, C.; Jones, S., 2017: Protocol for an online randomised controlled trial to evaluate the clinical and cost-effectiveness of a peer-supported self-management intervention for relatives of people with psychosis or bipolar disorder: Relatives Education And Coping Toolkit (REACT). Label="INTRODUCTION">Despite clinical guidelines recommendations, many relatives of people with psychosis or bipolar disorder do not currently receive the support they need. Online information and support may offer a solution.Label=&q...

Jordhoy, M.S.; Axelsson, B.; Ahlner Elmqvist, M.; Groenvold, M.; Kaasa, S., 2002: Organisation of palliative care A Scandinavian perspective. International Journal of Cancer Supplement (13): 22-23