geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Clinical usefulness and limitations of serum thyrotropin measurement by 'ultrasensitive' methods. Comparisons of five kits


, : Clinical usefulness and limitations of serum thyrotropin measurement by 'ultrasensitive' methods. Comparisons of five kits. Hormone Research 26(1-4): 105-117

Five different ultrasensitive thyrotropin (TSH) assay kits (Boots-Celltech, Immunotech, ORIS-CIS, Travenol and Boehringer) have been used for TSH measurements in various conditions. All the kits were based on an immunometric method but differed with regard to components and procedure. The sensitivity appeared essentially the same for the five kits (0.10 microU/ml) as well as the intraassay precision (coefficient of variation less than 12%). In contrast, the interassay coefficients of variation in the low TSH range varied from 12.8 to 21.3%. Discrepancies from kit to kit were observed and accounted for by differences in the components and procedure of the kits. Basal serum TSH was determined in normal subjects (n = 261) and in patients with thyroid dysfunction (n = 392). No overlap was shown between normals and patients with overt hypothyroidism. In contrast, an overlap existed between normals and hyperthyroids for all the kits but one. Measurements in patients with nontoxic goiter showed that TSH may be undetectable in clinically euthyroid patients, whatever the kit used. After TRH stimulation, 95% of the 375 patients tested associated either an absence of response to TRH with undetectable basal TSH values, or a blunted response with low basal TSH levels or normal response with normal basal TSH concentrations. However, 9 patients with suppressed TSH showed a response to TRH and 7 patients with normal basal TSH levels presented an exaggerated response to TRH. Taken together, these results demonstrate that even though ultrasensitive measurements of TSH do not meet the expectation of completely discriminating euthyroid from hyperthyroid patients, ultrasensitive TSH assay kits represent a powerful tool in the diagnosis of thyroid dysfunction, which would eliminate, in most instances, the need for TRH test and diminish thyroid hormone assay requests.

US$29.90

PMID: 3110041


Other references

Finochietto, R., 1955: Enrique Finochietto and our thoracic surgery. El Dia Medico 27(81): 2676-2680

Cooper, H.W.; Krawiec, J.F.; Dodson, D.E., 1959: What automation does for laundry service. Modern Hospital 92(6): 150 Passim-150 Passim

Harbach R.E.; Larsen J.R., 1976: Ultrastructure of sensilla on the distal antennal segment of adult oncopeltus fasciatus hemiptera heteroptera lygaeidae. International Journal of Insect Morphology & Embryology 5(1): 23-33

Shokes, F.M.; Lyda, S.D.; Jordan, W.R., 1977: Effect of water potential on the growth and survival of macrophomina phaseolina. A vapor-equilibration technique was developed to study the effect of growth-medium water potential on growth of M. phaseolina [root and stem rot pathogen]. Maximum growth at 30.degree. C was attained at water potentials near -17 bars, with reduced...

Carrillo, J.Antonio.; González, Jé.Antonio.; Gervasini, G.; López, R.; Fernández, Mía.Angeles.; Núñez, G.Martín., 2004: Thrombocytopenia and fatality associated with olanzapine. We report on a patient with fibromuscular dysplasia who presented with a right-sided giant calcified cavernous internal carotid artery (ICA) aneurysm and two additional supraophthalmic ICA aneurysms. Endovascular closure of the right ICA using det...

Wang, Y.; Huang, T.; Xie, L.; Liu, L., 2017: Integrative analysis of methylation and transcriptional profiles to predict aging and construct aging specific cross-tissue networks. Aging is a complex process relating multi-scale omics data. Finding key age markers in normal tissues could help to provide reliable aging predictions in human. However, predicting age based on multi-omics data with both accuracy and informative b...

Rataj, R., 1964: Complications In Tabetic Spondylopathy. Polish Medical Science and History Bulletin 7: 164-166

Anonymous, 2005: Transition to standardized drug concentrations by the end of 2008. Joint Commission Perspectives. Joint Commission on Accreditation of Healthcare Organizations 25(4): 3, 5-3, 5

Tienthai, P., 2005: An immunolohistochemical study of anti-apoptotic Bcl-2 in the pig's oviduct during the oestrous cycle. The purpose of this study was to determine the localization of Bcl-2 protein in porcine oviducts. Anti-human Bcl-2 monoclonal antibody was used to localize Bcl-2 protein immunohistochemically in the uterotubal junction (UTJ), isthmus and ampulla o...

King, T.A.; Cable, J., 2007: Experimental infections of the monogenean Gyrodactylus turnbulli indicate that it is not a strict specialist. Parasites represent a threat to endangered fish species, particularly when the parasite can host switch and the new host is vulnerable. If the parasite is highly host specific then successful host switching should be a rare occurrence; however, t...