+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cloning and expression of a cDNA encoding a novel human neurotrophic factor

, : Cloning and expression of a cDNA encoding a novel human neurotrophic factor. Febs Letters 266(1-2): 187-191

A cDNA encoding a novel human neurotrophic factor (designated nerve growth factor-2; NGF-2) was cloned from a human glioma cDNA library using a synthetic DNA corresponding to human nerve growth factor (NGF). The cloned cDNA encodes a polypeptide composed of 257 amino acid residues including a prepro-sequence of 138 residues and a mature region of 119 residues. The amino acid sequence of human NGF-2 exhibits 58% similarity with that of human NGF. Conditioned medium of COS-7 cells transfected with an expression plasmid for human NGF-2 cDNA supported the survival of sensory neurons isolated from dorsal root ganglia of embryonic chicks. A 1.5 kb of NGF-2 mRNA can be detected from an early development stage in rat brain, by Northern blotting analysis.


PMID: 2365067

Other references

Giobel, G., 1937: Some results of trials conducted by the Swedish Grassland Association on pasture leys with distribution of fertilizers during the growing period. Artificial N fertilizers should be applied in repeated liberal doses during the vegetative period; they are most effective under normal or slightly dry weather conditions, but particular circumstances should be studied in determining type and quan...

Pennypacker, C.S.; Sharpless, I., 1948: Clinical study of a new antihistaminic drug, thephorin. Pennsylvania Medical Journal 51(12): 1407-1411

Borovikov, A.M., 1985: Organizing the production and consumption of lumber for construction purposes. A discussion of the new Soviet code of practice TU 13-722-83 'Structural boards' and its practical and economic consequences. Two grades K24 and K19 are specified, each denoting the min. edgewise bending strength in MPa with probability...

Okrucká, A., 1996: Antithrombotic therapy in acute myocardial infarct. Thrombotic occlusion of coronary arteries is the reason of most acute coronary syndromes. A significant role in their prevention and therapy is taken by antiplatelet therapy. Acute coronary syndrome justifies also the use of anticoagulation therap...

Popov, A.M.; Bakai, A.F., 1989: Hydrochloric and mineral briquettes for cattle. Briquettes prepared as salt licks for cows in winter and summer and for sheep contained, respectively, NaCl 632, 695 and 503 kg/t; monocalcium phosphate 368, 0 and 423; disodium phosphate 0, 305 and 0; elemental sulphur 0, 0 and 74; CuSO4 0.28, 1....

Gani, M., 2001: Cividale dairy cooperative: an updated tradition. A brief historical account is given of a dairy cooperative founded in Cividale del Friuli, Italy, in 1924. Information is outlined on new initiatives and traditional products, including different cheeses.

Loban, K.M.; Ryzhkov, E.V., 1966: On the problem of vesicular dermatoses. Sovetskaia Meditsina 29(1): 135-139

Slavkin, H.C., 2000: The body's skin frontier and the challenges of wound healing: keloids. Journal of the American Dental Association 131(3): 362-365

Kozlowski, J.A.; Lowe, D.B.; Guzik, H.S.; Zhou, G.; Ruperto, V.B.; Duffy, R.A.; Mcquade, R.; Crosby, G., J.; Taylor, L.A.; Billard, W.; Binch, H., IIi; Lachowicz, J.E., 2000: Diphenyl sulfoxides as selective antagonists of the muscarinic M2 receptor. Structure-activity studies on (4-(phenylsulfonyl)phenyl)methylpiperazine led to the discovery of 4-cyclohexyl-alpha-(4-((4-methoxyphenyl)(S)-sulfinyl)phenyl)-1-piperazineacetonitrile, 1, an M2 selective muscarinic antagonist. Affinity at the clone...

Gangwere, SK. de Viedma, MG.; Llorente, V., 1986: Report on a red book of Iberian Orthoptera. Proceedings Triennial Meeting Pan American Acridological Society, 4: 129-148