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Cloning and expression of cDNA encoding 25-hydroxyvitamin D3 24-hydroxylase


, : Cloning and expression of cDNA encoding 25-hydroxyvitamin D3 24-hydroxylase. Febs Letters 278(2): 195-198

A cDNA encoding 25-hydroxyvitamin D3 24-hydroxylase (P450cc24) was isolated from a rat kidney cDNA library using specific antibodies to the enzyme. The isolated cDNA was 3.2 kbp long and contained a 1542-bp open reading frame encoding 514 amino acids. The deduced amino acid sequence contained a presequence typical of mitochondrial enzymes in the N-terminal region. The amino acid sequence shows less than 30% similarity to those of any other cytochrome P450s so far reported and, therefore, P450cc24 constitutes a novel family of P450. COS-7 cells transfected with the cDNA produced a protein that was reactive with the antibodies and catalyzed NADPH-dependent 24-hydroxylation of 25-hydroxyvitamin D3 in the presence of adrenodoxin and NADPH-adrenodoxin reductase. Using the cDNA as a probe we demonstrated that the increase of 24-hydroxylation activity caused by administration of vitamin D3 into rats was accompanied by an increase of the mRNA.

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PMID: 1991512


Other references

Stucke, K., 1950: Elastic behaviour of the tendon of Achilles in experimental rupture. Langenbecks Archiv für Klinische Chirurgie ... Vereinigt Mit Deutsche Zeitschrift für Chirurgie 264: 589-590

Bandarage, U.K.; Kuehne, M.E.; Glick, S.D., 1999: Total syntheses of racemic albifloranine and its anti-addictive congeners, including 18-methoxycoronaridine. Condensation of methyl 3-benzyl-1,2,3,4,5,6-hexahydroazepino(4,5-b)indole-5-carboxylate (12) with 4-(1,3-dioxolan-2-yl)-6-benzyloxyhexanal (11a) provided the tetracyclic intermediates methyl (3aSR,4RS,11bRS)-3-benzyl-2,3,3a,4,5,7-hexahydro-4-(2-ze...

Stratta, R.J.; Taylor, R.J.; Ozaki, C.F.; Bynon, J.S.; Miller, S.A.; Knight, T.F.; Fischer, J.L.; Neumann, T.V.; Wahl, T.O.; Duckworth, W.C., 1993: A comparative analysis of results and morbidity in type I diabetics undergoing preemptive versus postdialysis combined pancreas-kidney transplantation. Although combined pancreas-kidney transplantation (PKT) has become a valid treatment option for selected type I diabetics, the timing of PKT relative to the degree of nephropathy remains controversial. We analyzed results and morbidity in 30 type...

Alonso-Gonzalez, R.; Swan, L., 2014: Treating cardiac disease in pregnancy. Maternal cardiac disease is a very significant cause of both maternal morbidity and mortality in westernized countries. An effective treatment program must tackle all of the contributing components, including those due to public health issues, poo...

Paulová, M., 1993: Anatomy of the mammary gland and the physiology of breast feeding. Ceskoslovenska Pediatrie 48 Suppl 1: 13-14

Miura, N.; Takeda, A.; Terao, S.; Tanaka, H.; Ishikawa, S.; Mitsuma, T., 1996: Somatoparaphrenia caused by the lesion in the dominant cerebral hemisphere--a case report. We reported a rare case of cerebral infarction with somatoparaphrenia (SP) caused by involvement of the dominant cerebral hemisphere. The patient was 77-year-old right-handed woman who was noted to have atrial fibrillation, right hemiplegia, hemis...

Ertan, A.K.; Hendrik, H.J.; Tanriverdi, H.A.; Bechtold, M.; Schmidt, W., 2003: Fetomaternal Doppler sonography nomograms. To constitute Doppler flow velocity nomograms for use in obstetric clinics and to analyse the technical infrastructure of constructing Doppler sonography nomograms for clinical use. On a cross-sectional study plan basis 602 flow results of 370 pre...

Chong Soo C., 1970: Influence of ginseng d extract on the lymphoid tissues of normal mice and mice receiving pain or restraint stress. Journal of Catholic Medical College 19: 317-331

Krieg, I., 1975: Development of family and household structures in ten villages formerly settled by small farmers in the German Federal Republic--a contribution to social changes. Forschungsgesellschaft fur Agrarpolitik und Agrarsoziologie Schriftenreihe: 232

Ehgartner, E.M.; Langmann, G.; Langmann, A.; Lerchner, H.; Hanselmayer, H., 1987: Ultrastructure and histology in "healed" stromal herpes keratitis. Fortschritte der Ophthalmologie 84(6): 557-560