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Cloning of mitogen- and antigen-reactive B lymphocytes on filter paper discs. II. Paratope frequencies within the mitogen-selected repertoire

, : Cloning of mitogen- and antigen-reactive B lymphocytes on filter paper discs. II. Paratope frequencies within the mitogen-selected repertoire. Cellular Immunology 98(2): 506-516

Paratopic frequencies of C57BL/6 (Igh-Vb) and BALB/c (Igh-Va) mice were compared by determining the frequency of lipopolysaccharide-reactive, splenic B lymphocytes secreting antibody specific for (4-hydroxy-5-iodo-3-nitrophenyl) acetyl (NIP), trinitrophenyl (TNP), phosphorylcholine (PC), NIP/TNP, NIP/PC, and sheep erythrocytes. Despite the known genotypic and phenotypic differences between the two Igh-V loci, no significant differences in paratope frequencies were demonstrated. Similar determinations in C.B-20 mice, Ighb congenics of the BALB/c strain, and in C57BL/10 nude mice indicated that the mitogen-generated paratope frequencies directly reflected the capacity of immunoglobulin variable region elements rather than complex interactive or regulatory controls to generate diversity. We conclude that at least for the paratopic repertoire, the role of the somatic processes for the generation of antibody diversity exceeds the influence of germ-line differences between the Ighb and Igha haplotypes.


PMID: 3530507

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Giles, G.G., 1997: What do we know about risk factors for glioma?. Cancer Causes & Control 8(1): 3-4

Savchenko-Ye, M., 1975: Taxonomy and geographical distribution of the limoniid fly dicranoptycha mirabilis diptera limoniidae. The previously unknown males of D. mirabilis were found in one locality with the paratypical females (Turkmenia, Ispas on the Amu-Daryka river [USSR]). Overall they look like females, from which they differ only by a structure of the genital appar...

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Gurin, I.L., 1965: Angioma Of The Nasal Cavity From Arteries Of The Closing Type. Vestnik Otorinolaringologii 27: 45-49

Koriath, G., 1970: Development of performance of cattle twins reared under different conditions. The 32 pairs of dizygotic twins referred to in No. 1759 were used, the management, ages at 1st mating and seasons of calving being as in the previous experiment. Dairy performance was only slightly affected by age at 1st mating and season of calvi...

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Schmidt Nielsen, S.; Espeli, A., 1946: The bone marow in cattle and swine. It is well known to butchers that the marrow fat of meat animals is softer in the lower bones of the extremities than in the higher bones. To find the cause of this, the marrow fat of the humerus, ulna and radius, metacarpus, tibia and fibula, and...

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De-Mendonca-Lima, A.; Hartz, S., M.; Kindel, A., 2004: Foraging behavior of the white-browed (Basileuterus leucoblepharus) and the golden-crowned (B. culicivorus) warblers in a semidecidual forest in southern Brazil. This study intended to evaluate if differences occur in the foraging behavior of the Whitebrowed (Basileuterus leucoblepharus) and the Golden-crowned (B. culicivorus) warblers during different seasons (winter and summer). The variables evaluated w...

Robinson J.D., 1966: Conformational changes in brain microsomal membranes induced by specific nucleotides. Pharmacologist 8(2): 213