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ColIb plasmid genes that inhibit the replication of T5 and T7 bacteriophage

, : ColIb plasmid genes that inhibit the replication of T5 and T7 bacteriophage. Plasmid 20(3): 182-193

The colicin Ib (ColIb) plasmid genes that inhibit the replication of the T5-like and T7 bacteriophage have been cloned on an approximately 7200-bp ClaI fragment and their sites relative to each other and to the colicin immunity (imm) gene have been mapped. The inhibition of wild-type T7 by the clone is shown to be caused by the same gene or genes (pic) that cause the inhibition of T7 kinase-negative mutants and is a different gene than the one that causes inhibition of T5 (ibf or abi). The pic gene does not hybridize to the pif genes of the F plasmid that also cause the replication of T7 to be inhibited. The abi gene and the pic gene map very closely together but are under the control of different promoters. The abi gene has a maximum size of 900 bp and lies approximately 3000 bp away from the immunity gene, distal to the colicin gene. A site which maps in or near the gene binds very tightly to Escherichia coli RNA polymerase. The pic gene or genes lie between the abi gene and the imm gene and are contiguous with abi. Promoters for pic have been mapped and hypotheses to explain the inhibition of T7 by a cloned gene but not the whole ColIb plasmid are presented.


PMID: 3072576

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Haider, L.; Snabre, P.; Boynard, M., 2004: Ultrasound scattering from concentrated suspensions of aggregated red cells in shear flow. Ultrasound scattering technique is used to investigate dynamics of reversible fractal aggregates in dense suspensions and analyze shear break-up processes of Rayleigh fractal clusters. On the basis of an homogenous fractal flocculation and the hyb...

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Knight, Harry H., 1927: Acetropis americana, a new species of Miridae from Oregon. A. americana (p. 206), type in author's coll.

Taylor, B., 1980: An exclusive profile/interview of Richard S. Ross, MD, dean of the Johns Hopkins Medical Faculty and Vice President for Medicine. Interview by Blaine Taylor. Maryland State Medical Journal 29(1): 35-37

Wright C.P., 1971: Development of glufultyrless 1 1 1 en 7 a lethal mutant of drosophila melanogaster. Journal Of The Elisha Mitchell Scientific Society: 159-160

Bal, Bernard., 1996: Oxygastra curtisi at Roc de Chere provisional end to the enigma Oxygastra curtisi au Roc de Chere fin provisoire de lenigme. Sympetrum, 9: 27-29

Volpe, R.; Edmonds, M.; Lamki, L.; Clarke, P.V.; Row, V.V., 1972: The pathogenesis of Graves' disease. A disorder of delayed hypersensitivity?. Mayo Clinic Proceedings 47(11): 824-834