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Constitutive expression of exogenous c-myb gene causes maturation block in monocyte-macrophage differentiation


, : Constitutive expression of exogenous c-myb gene causes maturation block in monocyte-macrophage differentiation. Biochimica et Biophysica Acta 1088(3): 380-384

A nuclear protooncogene c-myb has been hypothesized to play an important role in hematopoiesis, but little is known about the physiological function of the c-myb gene products. To study the role of c-myb gene expression in monocyte-macrophage differentiation and proliferation, we introduced exogenous c-myb gene into murine myelomonocytic leukemia WEHI-3B(D+) cells which can be induced to differentiate into mature monocytes with granulocyte-colony stimulation factor (G-CSF) and actinomycin D. Expression of the transfected gene was found to result in elevated levels of c-myb transcripts, which were not subject to normal down-regulation by differentiation induction. This constitutive expression of c-myb gene allowed the c-myb transfectants to differentiate into promonocytes with G-CSF and actinomycin D, but blocked further maturation from promonocytes to mature monocytes. It is concluded that normal down-regulation of c-myb gene expression during monocyte-macrophage differentiation is required for the maturation of promonocytes to mature monocytes.

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PMID: 1707676


Other references

Tregoning P.; Lambeck K.; McQueen H., 2000: Postglacial rebound near the Lambert Glacier. Geophysical Research Abstracts: 00.

Lin, F.; Goldman, J.M.; Cross, N.C.P., 1994: A comparison of the sensitivity of blood and bone marrow for the detection of minimal residual disease in chronic myeloid leukaemia. We have sought to determine whether peripheral blood or bone marrow is more sensitive for assessment of minimal residual disease in chronic myeloid leukaemia (CML). Contemporaneous blood and marrow specimens were taken from 21 patients at various...

Hwang, J.Ha.; Vaezy, S.; Martin, R.W.; Cho, M-Yon.; Noble, M.L.; Crum, L.A.; Kimmey, M.B., 2003: High-intensity focused US: a potential new treatment for GI bleeding. High-intensity focused US has been shown to achieve hemostasis in lacerated large veins and arteries. High-intensity focused US was studied as a potential endoscopic treatment for GI bleeding. A segment of the auricular vein of the rabbit was lace...

Johnson, H.-W.S.erbeck, J., 1986: Oral history. Mr. Johnson discusses agricultural research in the United States as it relates to agronomy and plant genetics.

Coltorti M.; Ollier C.D., 2000: Geomorphic and tectonic evolution of the Ecuadorian Andes. In Early Miocene times the Cordillera did not exist and the Pacific Ocean reached the Oriente. In the Middle Miocene, the uplift of an elongated swell, consisting of Palaeozoic and older rocks, created the Eastern Cordillera. Decollements were act...

Kovacs M.; Turcsanyi G.; Tuba Z.; Wolcsanszky S.E.; Vasarhelyi T.; Dely Draskovits A.; Toth S.; Koltay A.; Kaszab L.; E.A., 1989: The decay of reed in hungarian lakes. Salanki, J And S Herodek (Ed ) Symposia Biologica Hungarica, Vol 38 Conservation And Management Of Lakes; Third International Conference, Keszthely, Hungary, September 11-17, 1988 Xi+645p Akademiai Kiado: Budapest, Hungary Illus Maps 461-472

Löllgen, H., 2011: Not Available. Mmw Fortschritte der Medizin 153(12): 29-33

Seahorn, T.; Cornick, J.; Cohen, N., 1999: Prognostic indicators for horses with duodenitis-proximal jejunitis: 75 horses (1985-1989). Journal of veterinary internal medicine 6(6): 307-311

Adams, T.E.; Epa, V.C.; Garrett, T.P.; Ward, C.W., 2000: Structure and function of the type 1 insulin-like growth factor receptor. A review. The protein structure, ligand binding and functions of type 1 insulin-like growth factor receptor are described, the effects of mutations and the role of this receptor in apoptosis, signal transduction, proliferation and differentiation...

Jansson, K.N.; Glasser, N.F., 2008: Modification of peripheral mountain ranges by former ice sheets; the Brecon Beacons, southern UK. The Brecon Beacons in southern Wales is a large upland area (900 km2 ) close to the periphery of the former British-Irish Ice Sheet. The geomorphology of the Brecon Beacons highlands and surrounding areas was mapped using satellite imagery and a d...