geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Culture of human fetal ovarian epithelium in a chemically-defined, serum-free medium: a model for ovarian carcinogenesis


, : Culture of human fetal ovarian epithelium in a chemically-defined, serum-free medium: a model for ovarian carcinogenesis. Anticancer Research 10(5a): 1233-1240

We report on of the possibility to culture normal human fetal ovarian epithelium in vitro under chemically defined conditions. Cell growth was dependent upon the presence of epidermal growth factor, insulin, and hydrocortisone in the culture medium but was unaffected by the presence of estrogen, progesterone, follicle stimulating hormone, or Müllerian inhibiting Substance. Growth could be maintained for up to 6 passages in culture. The possibility of culturing these cells in serum-free medium may represent an attractive model to investigate the biology of normal ovarian epithelium as well as its role in human ovarian tumorigenesis.

US$29.90

PMID: 1700663


Other references

Kittlesen, D.J.; Brown, L.R.; Braciale, V.L.; Sambrook, J.P.; Gething, M.J.; Braciale, T.J., 1993: Presentation of newly synthesized glycoproteins to CD4+ T lymphocytes. An analysis using influenza hemagglutinin transport mutants. Human lymphoblastoid cells transiently expressing the hemagglutinin (HA) glycoprotein of influenza virus are rapidly and efficiently recognized by CD4+ HA-specific T lymphocytes. This endogenous presentation pathway is sensitive to chloroquine and...

Anonymous, 1988: Examining surgeons. Lancet 2(8619): 1078-1078

Pecherstorfer, M.; Zimmer Roth, I.; Ludwig, H., 1992: Tumor associated anemia in squamous cell carcinoma Treatment with recombinant human erythropoietin. Annals of Hematology 65(SUPPL ): A102

Rosen, D.; Nichols, L.; Helms, D.; Rother, J.; Atkins, L.; Schieber, S.; Van D.W.ter, P.; Jones, S.; Goodman, J.; Leach, D., 1995: The future of employment-based health benefits. Ebri Issue Brief: 1-31

Karlson, A.G.; Martin, J.K.; Harrington, R., 1964: Identification Of Mycobacterium Tuberculosis With One Tube Of Liquid Medium. Qf first importance in the diagnosis and mangement of tuberculosis, Konno's test for niacin permits identification of M. tuberculosis by an in vitro procedure which, in comparison to animal pathogenicity tests, is relatively inexpensive and r...

ter Haar, E.; Hamel, E.; Balachandran, R.; Day, B.W., 1997: Cellular targets of the anti-breast cancer agent Z-1,1-dichloro-2,3-diphenylcyclopropane: type II estrogen binding sites and tubulin. Z-1,1-dichloro-2,3-diphenylcyclopropane (a.k.a. Analog II, A-II) is a known anti-breast cancer agent with apparent antiestrogenic effects and remarkably low toxicity in rodents. We have recently shown that A-II and its major metabolite Z-alpha-chl...

Slijepcevic, P.; Al-Wahiby, S., 2005: Telomere biology: integrating chromosomal end protection with DNA damage response. Telomeres play the key protective role at chromosomes. Many studies indicate that loss of telomere function causes activation of DNA damage response. Here, we review evidence supporting interdependence between telomere maintenance and DNA damage r...

Hutson R.W.; Dowding C.H., 1987: Microcomputer control of direct shear tests. Proceedings - Symposium on Rock Mechanics 28: 125-132

Lottes, M.T.; Henderson, H.Z., 1979: The relation of sucrose consumption to dental caries in a sample of Indianapolis children. Journal 58(1): 22-25

Gordon, R.D., 2000: South American Coccinellidae (Coleoptera). Part VII: A systematic revision of South American Scymnus (Pullus) Mulsant (Scymninae: Scymnini). Taxonomic revisions for the South American Scymnus species are presented. A key to the species, illustrations, synonymies and descriptions are provided.