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DAPI-DNA cytofluorometric study of glioma cells--application of DAPI-DNA cytofluorometry to paraffin embedded archival glioma tissue for nuclear DNA content analysis

, : DAPI-DNA cytofluorometric study of glioma cells--application of DAPI-DNA cytofluorometry to paraffin embedded archival glioma tissue for nuclear DNA content analysis. No to Shinkei 42(4): 352-359

Using DAPI-DNA cytofluorometry, the author analyzed nuclear DNA content of formalin fixed, paraffin embedded, glioma material obtained from 14 glioma cases at surgery. Sections of 10 microns were deparaffinized. Following simultaneous DAPI (4,6-diamidino-2-phenylindole dihydroporphyrin chloride)/HP (hematoporphyrin) staining, DAPI binds DNA and DNA-DAPI complexes emit blue fluorescence when exited by ultraviolet (UV) light. Through Zeiss fluorescence microscope, the author measured nuclear fluorescence intensity with histological verification of glioma cells. A DNA histogram was obtained with fluorescence intensity recorded on the abscissa and number of cells plotted on the ordinate. Samples of 20 normal non-neoplastic astrocytes taken from apparently normal brain tissue included in the histological slide were used as diploid (2 C) control. Based on DNA content, tumor cells were classified into 4 groups: N-group composed of cells with 2 C DNA content (normoploid), S-group with less than 2 C (hypoploid), L-group more than 4 C (hypertetraploid), I-group between 2 C and 4 C (intermediate ploidy). Intermediate ploidy was significantly higher and normoploid was significantly lower in glioblastoma compared with those of benign astrocytoma. Thus, DNA content and histological malignancy were well correlated. Due to limitation of measuring diaphragm of turret in the microscope, some extra large cell could not be included in it and was excluded from the measurement.


PMID: 1697181

Other references

Paone A., 2004: Evidence of crustal contamination, sediment, and fluid components in the Campanian volcanic rocks; a geochemical overview to explain the magmatic origin and the relationship between the mantle source and crustal effects in the Campanian volcanic subprovince. Pages 50-52 2004

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Behrendt, N.; Rønne, E.; Ploug, M.; Petri, T.; Løber, D.; Nielsen, L.S.; Schleuning, W.D.; Blasi, F.; Appella, E.; Danø, K., 1990: The human receptor for urokinase plasminogen activator. NH2-terminal amino acid sequence and glycosylation variants. The receptor for human urokinase-type plasminogen activator (u-PA) was purified from phorbol 12-myristate 13-acetate-stimulated U937 cells by temperature-induced phase separation of detergent extracts, followed by affinity chromatography with immo...

Vasil' ev, N., 1986: Land improvement - an important part of intensification of agriculture. There are 19.7 Mha of irrigated land and 14.6 Mha of drained land in the USSR. The improved land area is constantly being expanded so that, by 1990, a harvest of 32 Mt of grain and 80 000 Mt of fodder units is expected from this land. The article...

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Amin, H.M.; Lietz, H.; Hoppensteadt, D.A.; Walenga, J.M.; George, M.; Ahmad, S.; Fareed, J., 1998: Immuno-quantitation of soluble P-selectin in human plasma Effect of anticoagulant matrix and preliminary clinical data. Clinical Chemistry 44(6 PART 2): A51, June

Skripkina, G.I.; Garifullina, A.Zh., 2016: Serial clinical examinations as the main approach to dental caries prevention in children. Leading scientific and organizational prerequisites for the feasibility of clinical examination of the entire child population of the Russian Federation to the dentist is, above all, the high prevalence and intensity of dental diseases in children...

Waterhouse J.D., 1984: Investigation of pollution of the karstic aquifer of the Mount Gambier area in South Australia. 1: 202-205 1984

Abraham, D.; Leon, O.; Leon, S.; Lustigman, S., 2000: Development of a recombinant antigen vaccine against infection with the filarial worm Onchocerca volvulus. Efforts to control Onchocerca volvulus, the aetiologic agent of river blindness, have been limited to vector control and drug treatment to eliminate microfilariae, with no means available to prevent infection. The goal of this study was to develop...

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