geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

DNA degradation in terminally differentiating lens fiber cells from chick embryos


, : DNA degradation in terminally differentiating lens fiber cells from chick embryos. Proceedings of the National Academy of Sciences of the United States of America 74(12): 5579-5583

During the terminal differentiation of lens fiber cells, nuclear DNA is known to accumulate free 3'-OH ends and is progressively lost from the nucleus. Toward the end of this process, nuclei undergo pycnosis and disappear. The size of the DNA in the epithelia and in early and late stages of fiber cell development was examined by electrophoresis on nondenaturing agarose/polyacrylamide gels. Low molecular weight DNA of discrete sizes appears only at the final stages of nuclear degeneration in central fiber cells and persists after the disappearance of the nuclei. These low molecular weight DNA fragments appear as multiples of a monomeric unit and are similar to the fragments produced by the digestion of epithelial cell nuclei by micrococcal nuclease. The data indicate that in lens fiber nuclei the double-strand breaks in vivo affect the chromatin during nuclear degeneration, and the data suggest that the DNA of these cells is organized into chromatin composed of discrete subunits.

US$29.90

PMID: 271985


Other references

Daniels, W.; Miller, A., 2002: I-BEAM--an innovative building air quality software tool. Applied Occupational and Environmental Hygiene 17(6): 406-408

Messerschmitt, J., 1954: Perivascular disposition of plasmocytes of the bone marrow. Revue D'hematologie 9(2): 198-200

Schwartz, D.L.; Sosa, A.; Chun, S.G.; Ding, C.; Xie, X-Jin.; Nedzi, L.A.; Timmerman, R.D.; Sumer, B.D., 2017: SBRT for early-stage glottic larynx cancer-Initial clinical outcomes from a phase I clinical trial. To confirm safety and feasibility of hypofractionated SBRT for early-stage glottic laryngeal cancer. Twenty consecutive patients with cTis-T2N0M0 carcinoma of glottic larynx were enrolled. Patients entered dose-fractionation cohorts of incremental...

Chen, C.; Gibson, P., 1974: Seed development following matings of Trifolium nigrescens X Trifolium occidentale at different ploidy levels. Crop science: 14 (1) 72-77

Tucker H., 1990: Non steroidal anti androgens in the treatment of prostate cancer. Drugs of the Future 15(3): 255-265

Marsico, S.; Pizzo, A.; Mancuso, A.; Sodo, G., 1986: Maternal and cord prolactin during labor and delivery. Minerva Ginecologica 38(1-2): 19-25

Núñez, I.G.; Jidy, M.D.; Núñez, F.A.; Díaz, O.M., 2005: Echinococcus granulosus infection (hydatid cyst): a case report. Larval Echinococcus granulosus infection (hydatid cyst) was reported in a 10 years-old Ukranian girl. The clinical manifestations were slight since she only complained of frequent abdominal pain. The diagnosis was made by ultrasound and immunoenzy...

Bohme, H.; Oslage, H.J., 1986: Studies on the efficiency of salinomycin sodium as growth promoter for fattening pigs. During growth from 20 to 100 kg liveweight 156 SPF German Landrace boars were given a control diet or salinomycin, olaquindox or tylosin as growth promoters. Salinomycin was given at 80 or 50 mg/kg during the growing period, at 40 or 25 mg/kg duri...

Barone, R., 1980: The World Association of Veterinary Anatomists: 1955-1980. Anatomia, Histologia, Embryologia 9(3): 280-288

Watt, C.B.; Yang, J.H.; Jones, B.W.; Shaw, M.V.; Marc, R.E., 2002: Circuitry in the Rabbit Inner Plexiform Layer Enhanced Analysis with Deep-Etch EM Immunogold. Purpose: Our goals involve deciphering multiple inhibitory feedforward and feedback circuits that shape ganglion cell receptive fields. We sought to develop high-performance tools for complete immunolabeling of neuronal and glial cohorts in the ma...