geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Detection of viral and chlamydial antigens in open-lung biopsy specimens


, : Detection of viral and chlamydial antigens in open-lung biopsy specimens. American Journal of Clinical Pathology 83(3): 371-374

The recovery of viruses and Chlamydia trachomatis from cell cultures and the detection of their antigens in impression smears prepared from open-lung biopsy (OLB) specimens from immunocompromised adults were compared. Touch impression smears were prepared on three slides, each containing eight wells. OLB tissue was homogenized (Stomacher) and inoculated into MRC-5, primary monkey kidney, and McCoy cell cultures. The direct and indirect immunofluorescence (IF) tests were used to detect antigens to the following organisms: herpes simplex virus, adenovirus, parainfluenza types 1 and 3, respiratory syncytial virus, cytomegalovirus (CMV), Chlamydia trachomatis, influenza types A and B, and varicella-zoster virus. Of 105 OLB specimens, 21 viral isolates (20%) were recovered in cell culture; 20 were CMV and one was an influenza virus type A (H3N2). Both culture and IF results were positive with 12 specimens, but in nine instances a virus was isolated and IF was negative or eight times culture results did not yield the organism but IF test results were positive. Chlamydia trachomatis was never isolated or detected by IF. The authors recommend that for optimal detection of CMV from OLB specimens a new rapid centrifugation-enhanced cell culture system be used in preference, or in conjunction with a preliminary IF screen of impression smears for CMV detection.

US$29.90

PMID: 2983526


Other references

Keep, R.F.; Zhou, N.; Xiang, J.; Andjelkovic, A.V.; Hua, Y.; Xi, G., 2014: Vascular disruption and blood-brain barrier dysfunction in intracerebral hemorrhage. This article reviews current knowledge of the mechanisms underlying the initial hemorrhage and secondary blood-brain barrier (BBB) dysfunction in primary spontaneous intracerebral hemorrhage (ICH) in adults. Multiple etiologies are associated with...

Aletsee, Ludwig, 1959: On the history of the moors and forests of northern Holstein. Late glacial and post-glacial sediments and moor formations were investigated stratigraphically and by pollen analyses in 3 localities of northern Holstein in the vicinity of Neumunster-Nortorf. The value of the traditional pollen-floristic horizo...

Ehrmann, G.; Wiedmann, A., 1952: Experimental studies on animals with Candida albicans infection of the nails. The results of inoculation experiments on the nails of rats and guineapigs with cultures of Candida albicans isolated from human cases of onychomycosis are reported from the University Clinic for Skin Diseases, Vienna. The changes thus induced wer...

Leep, R.H., 1975: Effect of soil heavy metal contamination upon growth and nutrient composition of corn. In pot trials maize was given 0.05-2.0 meq Cd or Cr or 0.1-0.9 meq Ni or Zn. Application of Cd delayed emergence, stunted plants and decreased plant dry weight; plant Cd content increased with increased rate of applied Cd. Application of Cr signif...

Gomersall, C.D.; Joynt, G.M., 1996: Near-infrared spectroscopy and cerebral hemodynamics. Critical Care Medicine 24(8): 1423; Author Reply 1424-5

Bhat, V.M., 1949: Three Cases of iEncephalitis Lethargica. Indian Medical Gazette 84(11): 485-488

Tonge, W.C., 1931: Cuokoo-Shrikes at Eltham. Victorian Naturalist, 47: 194

Repetto, L.; Pace, M.; Mammoliti, S.; Bruzzone, M.; Chiara, S.; Oliva, C.; Guido, T.; Conte, P.F.anco; Campora, E., 1993: The impact of received dose intensity on the outcome of advanced ovarian cancer. It has been demonstrated that the prognosis of ovarian cancer is influenced by the dose intensity of cytotoxic treatment. The impact of received dose intensity of platinum-based combination chemotherapy on disease outcome was analysed in 226 stage...

Schäfer, C.; Friedrich, Bärbel.; Lenz, O., 2014: Novel, oxygen-insensitive group 5 [NiFe]-hydrogenase in Ralstonia eutropha. Recently, a novel group of [NiFe]-hydrogenases has been defined that appear to have a great impact in the global hydrogen cycle. This so-called group 5 [NiFe]-hydrogenase is widespread in soil-living actinobacteria and can oxidize molecular hydrog...

Kaiser, M.G.; Lamont, S.J., 2001: Genetic line differences in survival and pathogen load in young layer chicks after Salmonella enterica serovar enteritidis exposure. Early infection may result in long-term colonization of layers with Salmonella enterica sv. enteritidis (S. enteritidis, SE), resulting in shedding into table or hatching eggs. To evaluate genetic factors underlying early response to SE, genetic l...