geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Disproportional expression of proteolipid protein and DM-20 in the X-linked, dysmyelinating shaking pup mutant


, : Disproportional expression of proteolipid protein and DM-20 in the X-linked, dysmyelinating shaking pup mutant. Journal of Neurochemistry 49(6): 1912-1917

The shaking pup is an X-linked canine mutant with a severe hypomyelination of the CNS. Proteolipid protein (PLP) and the related DM-20 protein were examined in this mutant by densitometric scanning of Western blots stained with PLP antiserum. In the spinal cord of 4-week-old mutants, PLP was reduced to less than 1% of the control level, which is a greater deficiency than was found for other myelin proteins. On Western blots of control spinal cord, PLP stained much more intensely than DM-20. However, on Western blots of the mutant spinal cord, a component with the electrophoretic mobility of DM-20 stained slightly more intensely with PLP antiserum than PLP itself. This component was shown to be DM-20 by its lack of reactivity with an antiserum raised to a synthetic peptide corresponding to part of the PLP sequence that is missing in DM-20. Thus PLP and DM-20 are expressed in approximately equal and greatly reduced amounts in the mutant spinal cord. Although PLP or DM-20 could not be detected in brain from the 4-week-old mutant, similar disproportional expression of these two proteins was demonstrated in both spinal cord and brain from a 10-week-old mutant pup. Immunostaining of tissue sections showed that the small amounts of PLP and/or DM-20 synthesized in the mutant are present in the thin myelin sheaths. The results suggest that the shaking pup could have a primary defect in the PLP gene leading to a severe deficiency of PLP and DM-20 as well as disproportional expression of these two proteins.

US$29.90

PMID: 2445922


Other references

Tejeda-Dominguez, F.; Huerta-Cantillo, J.; Chavez-Dueñas, L.; Navarro-Garcia, F., 2017: A Novel Mechanism for Protein Delivery by the Type 3 Secretion System for Extracellularly Secreted Proteins. The type 3 secretion system (T3SS) is essential for bacterial virulence through delivering effector proteins directly into the host cytosol. Here, we identified an alternative delivery mechanism of virulence factors mediated by the T3SS, which con...

Pironcheva, G.; Todorov, I.; Lesseva, M.; Russev, G., 1988: U4 and u6 small nuclear ribonucleoprotein particles 2. evidence for their involvement in pre messenger rna splicing. The in vitro splicing of pre-mRNA of the human .beta.-globin gene in the presence of HeLa cell nuclear extract was investigated. Splicing was inhibited by auto-antibodies against U4 and U6 snRNP particles. No intermediates or products of the splic...

Endres D.; Krutzik S.; Mueller J.; Broughton A., 1982: Validation of a carboxyl terminal parathyroid hormone radio immunoassay. Clinical Chemistry 28(7): 1624

Tsuboya Koroku, 1950: Talc deposit of Azai Mine, Okayama prefecture. The talc deposits of the Azai mine area, Japan, occur in phyllite or sandstone. Chemical analyses and data on reserves are given.

English, C.W., 1985: Who'll pay for retirees' health plans?. U.S. News & World Report 99(17): 72-72

Berrocal, F.; Carreras, J., 1987: Metabolism of 2 3 bisphosphoglycerate xi. essential amino acids of pig phosphoglycerate mutase isozymes. Phosphoglycerate mutase isozymes (types M, B and MB) from pig tissues are inactivated upon treatment with reagents specific for histidyl, arginyl and lysyl residues. Their mutase, 2,3-biphosphoglycerate synthase and 2,3-bisphosphoglycerate phospha...

Sivaramakrishnan M.; Tucker S.D.; Klostergaard J.; Lopez Berestein G., 1991: Evidence for nitric oxide independent effector mechanism for macrophage mediated mitochondrial respiration inhibition. Proceedings of the American Association for Cancer Research Annual Meeting 32: 266

Koetsawang, S.; Omran, K.F.; Wallman, J.A., 1975: Laparoscopic sterilization using spring-loaded clips: early experience in Bangkok, Thailand. Advances in Planned Parenthood 10(2): 82-89

Tracchi, G.; Ballasso, G.; Magli, G., 1998: Oxadiargyl, a new herbicide for sunflower and vegetable crops. Field experiments were conducted to determine the effect different rates of oxadiargyl, a new herbicide which controls weeds on sunflowers and transplanted vegetables in Italy. Oxadiargyl applied at 300-400 g a.i/ha during pre-emergence or pre-tra...

Gemma Carr; Rob B Potter, 2012: Towards effective water reuse drivers, challenges and strategies shaping the organisational management of reclaimed water in Jordan. Treated wastewater or reclaimed water is gaining recognition as a valuable water resource around the world. To assess why, where and how water reuse takes place in Jordan, semi-structured interviews were conducted with representatives of 29 key or...