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Double isotopic method using dansyl chloride for the determination of GABA in rat C6 astrocytoma cell cultures

, : Double isotopic method using dansyl chloride for the determination of GABA in rat C6 astrocytoma cell cultures. Journal of Neuroscience Research 16(3): 561-566

Methods are described for the quantitative measurement of GABA in culture. The method can be adapted to any amino acid or dansyl-chloride-reactive species. The sensitivity and selectivity of the procedure result from the double isotopic design in which (14C)-labeled internal standard was added to the samples before reaction with (3M)-labeled dansyl chloride. Values obtained by ion-exchange amino acid analysis of cultures agree closely with the values obtained by the double isotopic method. This method is sensitive enough to measure GABA intracellularly and the condition medium.


PMID: 3772993

DOI: 10.1002/jnr.490160311

Other references

Johnson, O.E.; Okon, R.D., 2013: Perception and practice of female genital cutting in a rural community in southern Nigeria. This study was carried out to determine the awareness and practice of FGC in a rural community in southern Nigeria. A cross sectional study was carried out among Ayadehe women in Itu, LGA Akwa Ibom State, Nigeria using a semi-structured interviewe...

Grossman, A.I.; Cannizzaro, L.A.; Warmoth, L.A.; Rosenberg, R.N., 1987: An anonymous single copy clone (p1,2-2) from a flow sorted human chromosomes 1 and 2 library (D1S33). Nucleic Acids Research 15(14): 5904-5904

McDonough M.R.; Grover T.W.; McNicoll V.J., 1993: New insights on the geology of the early Proterozoic southern Taltson magmatic zone, northeastern Alberta. Program with Abstracts - Geological Association of Canada(Mineralogical Association of Canada(Canadian Geophysical Union, Joint Annual Meeting 1993: 68

Thorner J.; Fuller R.; Brake A., 1985: Yeast pheromone precursor convertase is a calcium dependent lysine arginine specific endopeptidase. Federation Proceedings 44(3): VIII

Schiller P., 1980: New advances in sex education. Forleo, R. Pasini (ed.). Medical Sexology; The 3rd International Conference, Rome, Italy, . Xx 644p. Elsevier North-Holland Biomedical Press: Amsterdam, Netherlands; Publishing Sciences Group: Usa. Illus. P466-476

Camoni, I.; Muccio, A. di; Fabbrini, R., 1991: Estimation of daily intake of pesticide residues from the Italian diet during 1986 to 1987. Using UNEP/FAO/WHO criteria and monitoring data daily intake of residues from the Italian diet during 1986-87 was estimated for about 50 pesticides and compared with acceptable daily intake (ADI) values. About 18 000 estimations were made on about...

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Brace W.F.; Scholz C.H.; LaMori P.N., 1969: Isothermal compressibility of kyanite, andalusite, and sillimanite from synthetic aggregates. A compressibility determination technique is described in which an unknown mineral, available only in small quantities and in finely divided form, is mixed with a ductile solid of known characteristics; from measurements on hot-pressed samples of...

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