geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effect of various anti-cancer drugs on human renal cell carcinoma serially transplanted into nude mice


, : Effect of various anti-cancer drugs on human renal cell carcinoma serially transplanted into nude mice. Hinyokika Kiyo. Acta Urologica Japonica 32(10): 1423-1441

Using two xenografts of human renal cell carcinomas serially transplanted in nude mice (AM-RC-1 and AM-RC-6), both of which maintained the basic histologic features of the original tumor and showed a constant growth rate, the effects of various anticancer agents against 2 target tumors were evaluated. MitomycinC (MMC), adriamycin (ADM), cisplatinum diaminodichloride (CDDP), 5-fluorouracil (5FU), 5-fluro-2'-deoxy-beta-uridine (FUDR) and human lymphoblastoid interferon (HLBI) against AM-RC-1, and MMC, ADM, CDDP, vinblastin (VBL) and etoposide (VP-16) against AM-RC-6. Drugs other than HLBI were administered 3 times in total every three to five days by intraperitoneal injection according to Battelle Columbus Laboratories Protocol and HLBI was injected daily for 10 days intraperitoneally. Anti-cancer effects were evaluated based on tumor growth curve and changes of histologic findings. In terms of tumor growth only MMC (in a dose of 3 mg/kg) revealed a statistically significant inhibitory effect against both AM-RC-1 and AM-RC-6 (respectively P less than 0.001 and P less than 0.05). Concerning AM-RC-1, a significant difference (P less than 0.01) was recognized in the ADM group (5 mg/kg) at the time of the second administration, but evaluation could not ultimately be done owing to appearance of acute toxity after the last dose. The most remarkable histologic changes by light microscopy were recognized in the MMC group (in a dose of 3 mg/kg) against AM-RC-1. They were degenerative findings such as intracellular and nuclear vacuolation, karyorrhexis, karyolysis, karyopyknosis and marginal hyperchromatosis, which corresponded to grade IIa of the classification of the National Cancer Center. The other drugs administered to AM-RC-1 exhibited only grade O to grade I changes. On the other hand, in AM-RC-6, histologic changes were mild (less than grade II) for all the drugs. Electron microscopic features were as follows. AM-RC-1: Marked increase of vacuole of organella was observed and lumens were filled with a large quantity of debris in MMC group (3 mg/kg). In the ADM group (5 mg/kg) there was debris in lumens, although almost no changes of organella were seen. CDDP groups (both 5.6 mg/kg and 2.8 mg/kg) showed autophagic vacuole in the cytoplasm and increased collagen fibers in the stroma but little changes of organella. AM-RC-6: Mild intracellular vacuolation was recognized in the MMC group (3 mg/kg). Watery degeneration and microfibrils were found in the cytoplasm in both ADM (5 mg/kg) and CDDP (5.6 mg/kg, 2.8 mg/kg) groups.

US$29.90

PMID: 3565179


Other references

Broker, H.; Clagett, G.Patrick., 2006: Vascular reconstruction using deep vein for limb length discrepancy in a child. Iatrogenic vascular injuries can result in claudication and limb length discrepancy in growing children. Traditional reconstruction has been performed with great saphenous vein as a conduit. We report the case of a 7-year-old boy with a symptomati...

Kuruvadi S., 1991: Estimation of rubber and resin content in guayule from a diverse breeding population in mexico. Thirty-eight accessions and two control varieties of guayule were evaluated under rain-fed conditions. The analysis of variance showed significant differences for percentage rubber. The differences between percentage resin, plant height, and top s...

Tuzlukov A.P.; Gorbatovskaya N.S., 1982: Role of cervical osteochondrosis in the clinical picture of hypertensive disease. Patients (160) with hypertensive disease were examined for cervical osteochondrosis. All the examined patients including young subjects manifested clinical and X-ray signs of cervical osteochondrosis. The latter was shown to have a noticeable effe...

Zhang, X.; Wang, X.; Zhang, Z., 2002: Accuracy of computerized aid diagnosis, surgical simulation and facial appearance prediction in orthognathic surgery. The purpose of this report is to evaluated the accuracy and potential advantages of the computer system in orthognathic surgery. The accuracy of computer prediction was evaluated in 31 patients by using the orthognathic surgery prediction expert s...

Guma, A.; Bashan, N.; Burdett, E.; Hundal, H.; Ramlal, T.; Klip, A., 1992: GLUT1 glucose transporter and the long-term regulation of glucose uptake. Journal of General Physiology 100(6): 54A

Piper, G.M.; Frank, B.; Thorner, R.M., 1965: Survey Of Home-Delivered Meals Programs. A prelimi-nary study by the Division of Chronic Diseases, Public Health Service, of operating home-delivered meals programs emphasizes the fact that the services are available to small numbers of people in scattered communities. Sixteen of the 22...

Wells, P., 1982: Identifying learning needs: a teaching for emergency procedures. Aorn Journal 36(4): 639-644

Sadhu, A.N.; Mahajan, R.K., 1985: Technological change and agricultural development in India. During recent years Indian agriculture has experienced rapid changes in production technology. There are fears, however, that with the new technology being limited to a few crops, it has been the main factor responsible for displacement of labour,...

Brooks, L.A.; Tidy, J.A.; Gusterson, B.; Hiller, L.; O'Nions, J.; Gasco, M.; Marin, M.C.; Farrell, P.J.; Kaelin, W.G.; Crook, T., 2001: Preferential retention of codon 72 arginine p53 in squamous cell carcinomas of the vulva occurs in cancers positive and negative for human papillomavirus. We have sought to determine the basis for preferential loss of the codon 72 proline (72P) rather than the arginine (72R) allele in squamous cell carcinoma of the vulva with loss of heterozygosity (LOH) in p53. The proportion of cases containing hu...

Ibrahim, M.Magdy.; Bond, J.; Bergeron, A.; Miller, K.J.; Ehanire, T.; Quiles, C.; Lorden, E.R.; Medina, M.A.; Fisher, M.; Klitzman, B.; Selim, M.Angelica.; Leong, K.W.; Levinson, H., 2015: A novel immune competent murine hypertrophic scar contracture model: a tool to elucidate disease mechanism and develop new therapies. Hypertrophic scar (HSc) contraction following burn injury causes contractures. Contractures are painful and disfiguring. Current therapies are marginally effective. To study pathogenesis and develop new therapies, a murine model is needed. We have...