+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of amino acids on acute alcohol intoxication in mice--concentrations of ethanol, acetaldehyde, acetate and acetone in blood and tissues

, : Effects of amino acids on acute alcohol intoxication in mice--concentrations of ethanol, acetaldehyde, acetate and acetone in blood and tissues. Arukoru Kenkyu to Yakubutsu Izon 25(5): 429-440

Condensation reactions between some SH-amino acids (L-and D-cysteine 1%) and acetaldehyde (50 microM) were studied in vitro experiment. In the aqueous solution, free acetaldehyde was reduced to 41.3% by L-cysteine and to 36.4% by D-cysteine. In the reaction with human blood medium, after the medium was deproteinized with perchloric acid reagent, acetaldehyde was reduced to 47.0% by L-cysteine and to 43.8% by D-cysteine. D-Cysteine appears to have great stability of reacting acetaldehyde. In vitro experiment reactability for D-cysteine exhibited 3-8% higher than that for L-cysteine. Next, effects of some amino acids on alcohol metabolism were studied in male ICR mice. The animals were given ethanol through a gastric catheter at a dose of 2 g/kg and they were intraperitoneally injected L-cysteine (300 mg/kg), D-cysteine (300 mg/kg), L-alanine (300 mg/kg) and control (saline), respectively in the period of one hour before the injection of ethanol. Blood and tissues samples were analyzed for ethanol, acetaldehyde, acetate and acetone during alcohol intoxication in mice by head space gas chromatography. In the groups administered D-cysteine and L-cysteine, the mice showed a definitely faster oxidation and disappearance of ethanol. Especially in the D-cysteine group, ethanol levels in blood, liver and brain remained lower than that in the other groups (p less than 0.01). Acetaldehyde levels in blood, liver and brain remained low by L-cysteine. Ethanol metabolites during alcohol oxidation by chemical reactabilities of L- and D-cysteine showed different distribution in the mice, respectively. In the mice received L-alanine, acetate and acetone levels in blood, liver and brain were distinctly reduced (p less than 0.01). L-Alanine is reported to supply an abundance of pyruvic acid that performs the NAD-generating system. NAD produced is introduced to alcohol metabolism and the TCA cycle. It was thus presumed that the L- or/and D-cysteine, and L-alanine was effective in acute alcohol intoxication by heavy drinking.


PMID: 2275637

Other references

Hourdillé, P.; Hasitz, M.; Belloc, F.; Nurden, A.T., 1985: Immunocytochemical study of the binding of fibrinogen and thrombospondin to ADP- and thrombin-stimulated human platelets. We have used immunogold staining to locate thrombospondin (TSP) on thrombin-activated human platelets, and have compared its distribution with that of fibrinogen (or fibrin) on thrombin- and ADP-stimulated platelets. To do this, isolated platelets...

Wolff, H., 1999: Intuition in surgery. Zentralblatt für Chirurgie 124 Suppl 3: 38-38

Marker, C.; Zemann, A.; Terhoerst, T.; Kiefmann, M.; Kastenmayer, J.P.; Green, P.; Bachellerie, J.P.erre; Brosius, J.; Huettenhofer, A., 2002: Experimental RNomics: Identification of 140 candidates for small non-messenger RNAs in the plant Arabidopsis thaliana. Background: Genomes from all organisms known to date express two types of RNA molecules: messenger RNAs (mRNAs), which are translated into proteins, and non-messenger RNAs, which function at the RNA level and do not serve as templates for translat...

Rodrigues, VM. de Moraes, RAO., 1981: Vespideos sociais - estudo de Polybia (Apopolybia) jurinei de Saussure, 1854 (Polistinae - Polybiini). Data are presented on the geographical distribution, nest structure and nesting behavior of P. jurinei. The time for a life cycle is estimated. The population of a colony polygyny were recorded. Queens presented a jelly-like material associated wi...

Cavaillon, J.M., 1982: The nature and mechanism of action of anti-caries vaccine. Revue D'odonto-Stomatologie 11(5): 357-360

Grossman, M.I., 1974: Candidate hormones of the gut. I. Introduction. Gastroenterology 67(4): 730-731

Rivière, I.; Roy, K., 2017: Perspectives on Manufacturing of High-Quality Cell Therapies. Molecular Therapy 25(5): 1067-1068

Cullen, P.; Clapham, K.; Lo, S.; Rogers, K.; Hunter, K.; Treacy, R.; Porykali, B.; Keay, L.; Senserrick, T.; Ivers, R., 2017: Communities driving change: evaluation of an Aboriginal driver licensing programme in Australia. The Driving Change programme was developed to facilitate access to licensing in Aboriginal communities in Australia. This process evaluation aimed to explore whether Driving Change was implemented as intended and was addressing the needs of the co...

Abraham, O.C.; Klein, J.; Chalasani, G.; Cassells, L.; Dansey, R.; Abella, S.; Karaness, C.; Peters, W.; Baynes, R.; Chandrasekar, P.H., 1998: Infectious complications in women undergoing autologous peripheral blood progenitor cell transplantation for breast cancer. Blood 92(10 SUPPL 1 PART 1-2): 320B, Nov 15

Tatagiba, F., C.P.; Alves, R.-Jose-Valka; Silva, B.-Rezende, 2004: Two new white-flowered species of Pitcairnia from Brazil. Two new white-flowered species of Pitcairnia (Bromeliaceae) from coastal Rio de Janeiro State, Brazil, are described.