+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Endostreptosin: isolation of the probable immunogen of acute post-streptococcal glomerulonephritis (PSGN)

, : Endostreptosin: isolation of the probable immunogen of acute post-streptococcal glomerulonephritis (PSGN). Clinical and Experimental Immunology 76(2): 198-203

It is now generally accepted that acute post-streptococcal glomerulonephritis (PSGN) is the consequence of the formation of antigen-antibody-complement complexes on the basement membrane of the glomerulus and that the antigen is of streptococcal origin. In cases of acute PSGN a high titre of specific antibodies to a streptococcal cytoplasmic extract can be found at the very beginning of the disease. This cytoplasmic antigen which we called endostreptosin (ESS) is probably the pathogenetic antigen of glomerulonephritis. It is deposited on the subendothelial side of the basement membrane in the first few days of the disease and is rapidly covered by newly-formed and specific antibody and complement with resultant immune injury causing signs and symptoms of symptomatic but also frequently asymptomatic acute glomerulonephritis. To further characterize and isolate ESS we used immunoaffinity chromatography and Western blotting techniques. PAGE analysis of the affinity-isolated ESS revealed the major component to have a molecular weight of approximately 45 kD. Sera from patients with PSGN or sera of rabbits immunized with affinity-isolated ESS reacted by Western blotting with at least one antigenic component with a molecular weight of approximately 45 kD. Normal human sera or the sera of non-immunized rabbits failed to demonstrate activity against this antigen. The basement membranes of the glomeruli of patients with very early PSGN stain with fluorescein-labelled gammaglobulin of patients with glomerulonephritis. This staining can be prevented when these sera are pre-absorbed with ESS but not by pre-absorption with intact cells or cytoplasmic extracts of other bacteria.


PMID: 2667818

Other references

Barakat, M.R., 2012: Some aspects of the problem of nutrition in Egypt. Journal of Tropical Medicine and Hygiene 50(5): 95-98

Knipp, G.T.; Audus, K.L.; Soares, M.J., 1999: Nutrient transport across the placenta. The placenta forms a selective barrier that functions to transport nutrients that are of critical use to the fetus. Nutrient transport across the placenta is regulated by many different active transporters found on the surface of both maternal and...

Sica, G.S.; D.C.rlo, S.; Biancone, L.; Gentileschi, P.; Pallone, F.; Gaspari, A.L., 2011: Single access laparoscopic ileocecal resection in complicated Crohn's disease. Surgical Innovation 17(4): 359-360

Jacobs, S.R.; Weiner, B.J.; Reeve, B.B.; Hofmann, D.A.; Christian, M.; Weinberger, M., 2016: Determining the predictors of innovation implementation in healthcare: a quantitative analysis of implementation effectiveness. The failure rates for implementing complex innovations in healthcare organizations are high. Estimates range from 30% to 90% depending on the scope of the organizational change involved, the definition of failure, and the criteria to judge it. The...

Binford, A.; Liljequist, L., 2008: Behavioral correlates of selected MMPI-2 clinical, content, and restructured clinical scales. In this study, we evaluated the validity of the Restructured Clinical (RC; Tellegen et al., 2003) scales by identifying and comparing behavioral correlates of selected RC scales (RCd, RC2, RC4), their original Clinical scale counterparts (Scale 2,...

Elzinga L.W.; Rosen S.; Coffman T.; Bennett W.M., 1991: Fish oil fo supplementation in a model of chronic cyclosporin csa nephrotoxicity ntx. Journal of the American Society of Nephrology 2(3): 661

Yasutake, T., 2003: Trend analysis of large-scale management by dynamics of agricultural structure. Changes in farm size are described, using data from the Japanese agricultural census in 1990 -2000. Farms maintaining a size of more than 5 ha represented 64.4%, but in the Shikoku area this was only 29.8%. Farms which grew from 3-5 ha to more tha...

Cloud, J.L.; Hoggan, K.; Belousov, E.; Cohen, S.; Brown-Elliott, B.A.; Mann, L.; Wilson, R.; Aldous, W.; Wallace, R.J.; Woods, G.L., 2005: Use of the MGB Eclipse system and SmartCycler PCR for differentiation of Mycobacterium chelonae and M. abscessus. Although accurate in the identification of Mycobacterium species, partial 16S rRNA gene sequencing does not distinguish Mycobacterium chelonae from M. abscessus. Thus, we designed a SmartCycler PCR assay targeting the 16S-to-23S internal transcrib...

Christlieb, A.R., 1982: Treating hypertension in the patient with diabetes mellitus. Medical Clinics of North America 66(6): 1373-1388

D.Lumley H., 1988: The stratigraphy of vallonnet cave france. Five stratigraphic sets show how Vallonnet Cave was filled, with the total depth of the cave not exceeding 1,5 metre. Stratigraphidc set I is made up of large fallen blocks and a stalagmite floor, and corresponds to the first continental filling....