geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Ensemble noise and current relaxation analysis of K+ current in single isolated salivary acinar cells from rat


, : Ensemble noise and current relaxation analysis of K+ current in single isolated salivary acinar cells from rat. Pflugers Archiv 406(1): 69-72

The K+ channel in rat parotid gland acinar cells were investigated by ensemble current noise analysis in single isolated cells employing the giga-seal whole cell current recording mode. Sets of 20-40 identical de- and hyperpolarization voltage steps were applied and the resultant current records were processed by computer to obtain the mean and the variance of the current. The time-course of the mean current could be fitted by the sum of two exponentials, suggesting a 3-state model. The simplest plausible hypothesis is a model with one open and two closed states. Assuming this model, the relationship between the variance (sigma 2) and the mean current (I) could be fitted by the function sigma 2/I = i--I/N. The estimated single channel i/V-relations were similar to those taken from single channel current recordings, and the size of the population of channels per cell (N) was 76 +/- 26 (n = 12). The validity of the model was tested by a successful simulation of the time-course of the variance.

US$29.90

PMID: 2419831


Other references

Preuss, Axel, 1998: Quo vadis, professional food control?. Lebensmittelchemie 52(1): 1-3

Roldan, E.R.; Fragio, C., 1993: Phospholipase A2 activation and subsequent exocytosis in the Ca2+/ionophore-induced acrosome reaction of ram spermatozoa. In ram spermatozoa treatment with Ca2+ and A23187 or ionomycin stimulated the release of arachidonic acid (20:4) and exocytosis of the acrosome in a time- and concentration-dependent manner. Diacylglycerol did not appear to be the source of 20:4....

Choi, D.Won.; Chang, Y.Hyuk., 2012: Steady and Dynamic Shear Rheological Properties of Buckwheat Starch-galactomannan Mixtures. This study investigated the effects of galacomannans (guar gum, tara gum, and locust bean gum) on the rheological properties of buckwheat starch pastes under steady and dynamic shear conditions. The power law and Casson models were applied to desc...

Williams, R.; Ross, A.; Sotos, P.C.eeks, C., 2000: Water-repellent preservative treatment of brick molding prior to factory priming improves paint service life. Proceedings annual meeting of the American Wood Preservers' Association7(97): 65-67

Baliga; Sooryakumar; Rao; Tipping; Poll, 1987: Delta J=4 infrared transitions of solid D2 and a Raman study of H2, D2, and HD in their liquid and solid phases. Physical Review. B, Condensed Matter 35(18): 9766-9770

Polanco M.C.; Pelaez M.I.; Ruiz M.L., 1988: Factors affecting callus and shoot formation from in vitro cultures of lens culinaris medik. The influence of culture medium and explant on callus and shoot formation of lentil (Lens culinaris Medik.) has been studied. Three different explants (shoot-tip, first node and first pair of leaves) from three Spanish lentil cultivars were cultiv...

Piquet, J.; Meignan, M.; Teisseire, B.; Harf, A., 1985: Heterogeneity of pulmonary ventilation/perfusion ratio: significance and measurement. La Revue du Praticien 35(17): 971-6, 979-81

Marshall, S.F., 1956: Technique of total gastrectomy. Surgical Clinics of North America 36(3): 673-685

Harris, R.E.; Christiansen, K.H.; Blunt, M.H., 1978: Observations on the epidemiology of malignant catarrhal fever. Geographical differences in the reported incidence of MCF in New Zealand are related to sheep/cattle and beef/dairy cattle ratios in the area. Apparent differences in age susceptibility may be due to differential reporting, but this point needs ex...

Heidrich, H.; Ott, H.; Beach, R.C., 1981: Lormetazepam - a benzodiazepine derivative without hangover effect? A double-blind study with chronic insomniacs in a general practice setting. Lormetazepam, a new short-acting benzodiazepine, was tested multicentrally in 15 general practices. The subjects were 62 chronically sleep-disturbed patients. The first 7 days served as a placebo baseline week, the next 14 days were either lormeta...