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Enzyme immunoassay of testosterone in plasma, with use of polyethylene glycol to separate antibody-bound and free hormone

, : Enzyme immunoassay of testosterone in plasma, with use of polyethylene glycol to separate antibody-bound and free hormone. Steroids 34(5): 575-579

We describe an enzyme immunoassay for testosterone in which we use a testosterone-3-(carboxymethyl)oxime horseradish peroxidase conjugate as the label and an antiserum, raised in rabbits, to testosterone-3-(carboxymethyl)oxime-bovine serum albumin conjugate. Polyethylene glycol (Carbowax 6000) is used to separate antibody-bound and free steroid. The assay has a sensitivity of 12 pg/assay tube and satisfies the usual criteria of specificity, precision and accuracy. The results agree well with those obtained with a comparable radioimmunoassay.


PMID: 390778

Other references

Ide H.; Fujiya S.; Aanuma Y.; Agishi Y., 1990: Effects of simvastatin an hmg coenzyme a reductase inhibitor on plasma lipids and steroid hormones. Seven hyperlipidemic postmenopausal women received 2.5 mg of simvastatin daily for six months, 5 mg for four months, and 2.5 mg for two months. During treatment, there were significant decreases in the patients' levels of plasma total cholest...

Lauber, A.H.; Romano, G.J.; Pfaff, D.W., 1991: Gene expression for estrogen and progesterone receptor mRNAs in rat brain and possible relations to sexually dimorphic functions. A clear neuroendocrine sex difference lies in the ability of the female rat to produce an ovulatory surge of luteinizing hormone. Preoptic neurons, as they respond to estrogen and progesterone, have been proven to be involved in this mechanism, wi...

Bush, T.G., 2002: Enteric glial cells. An upstream target for induction of necrotizing enterocolitis and Crohn's disease?. As a direct consequence of the sophisticated arrangement of its intrinsic neurons, the gastrointestinal tract is unique among peripheral organs, in its ability to mediate its own reflexes. Neurons of the enteric nervous system are intimately assoc...

Skld, M.K.; Cullheim, S.; Risling, M., 2002: INDUCTION OF HIF1alpha IN MOTONEURONS AFTER INTRASPINAL AXOTOMY. Tissues respond to hypoxia by upregulation and activation of hypoxia-inducible factors (HIFs); members of the basic helix loop helix (bHLH) superfamily of transcription factors. HIF, in turn, is dimerized and bind to hypoxia-response elements (HRE...

Scheihing M.H.; Gluskoter H.J., 1977: Kaolinite interstitial networks in pyrite framboids of the Meigs Creek coal of Ohio. Abstracts with Programs - Geological Society of America 9(7): 1159-1160

Macedo, C.F.; Tomaz, C., 1991: Water maze learning in rats with neocortical and hippocampal lesions. Experiments were carried out in order to determine whether groups of rats (N = 7) with unilateral or bilateral ablation of the neocortex or with removal of the neocortex plus the hippocampus of either hemisphere could learn to escape from a tank o...

Johnson, T.R.; Smerkers, B.; Moulder, J.K.; Stellar, J.R.; Febo, M., 2013: Neural processing of a cocaine-associated odor cue revealed by functional MRI in awake rats. Using an olfactory conditioning procedure, brain stimulation reward threshold measurements, and functional magnetic resonance imaging (fMRI), we investigated brain stimulation reward threshold change and fMRI neural activation in response to a coc...

Voute C., 1982: Consulting. ITC Journal = Bulletin de l'ITC 1982(1): 55-59

Sleigh M.A., 1990: Symposium cellular and molecular aspects of mucus and cilia. Biorheology 27(3-4): 527-532

Santiago, Mía.Jesús.; Ruiz-Rubio, M.; Dio, L.Di.; González-Reyes, J.A.; Alejandre-Durán, E., 2008: Ubiquitous expression of two translesion synthesis DNA polymerase genes in Arabidopsis. Cellular DNA is continually exposed to a large variety of external and internal DNA-damaging agents. Although lesions can be removed by different repair processes, damages often remain in the DNA during replication, and specialized DNA polymerase...