+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Epidemiologic typing of Enterobacter sakazakii in two neonatal nosocomial outbreaks

, : Epidemiologic typing of Enterobacter sakazakii in two neonatal nosocomial outbreaks. Diagnostic Microbiology and Infectious Disease 13(6): 467-472

Two unrelated hospital outbreaks of Enterobacter sakazakii, involving meningitis, bacteremia, and colonization of neonates, were investigated. In each of these outbreaks, E. sakazakii was isolated from both patients and dried infant formula. In previous outbreaks, the source and mode of transmission of E. sakazakii in neonatal infections was not determined. In this study, we used a combination of typing methods (plasmid analysis, antibiograms, chromosomal restriction endonuclease analysis, ribotyping, and multilocus enzyme electrophoresis) to evaluate the isolates from each outbreak as to their relatedness. The typing results differed among outbreaks, but in each one, patient and formula isolates shared the same typing pattern. The only exceptions were disk antibiograms, which often varied among colonies selected from each of the isolates. Plasmid analysis, chromosomal restriction endonuclease analysis, ribotyping, and multilocus enzyme electrophoresis all were effective as epidemiological typing methods for E. sakazakii, especially when used in combination. By using this typing scheme, we have confirmed that E. sakazakii from intrinsically contaminated dried infant formula was the source of neonatal infection.


PMID: 2279379

Other references

Gragnoli, G.; Signorini, A.M.; Tanganelli, I., 1984: Effects of ticlopidine on the plasma levels of beta-thromboglobulin and thromboxane B2 in types 1 and 2 diabetics. 11 insulin-dependent and 19 non insulin-dependent diabetics with varying degrees of metabolic compensation and with high plasma levels of beta-thromboglobulin and thromboxane B2 were selected. Depending on the type of diabetes and the degree of gl...

Rudi, A.; Talpir, R.; Kashman, Y.; Benayahu, Y.; Schleyer, M., 1993: Four new C16 1,2-dioxene-polyketides from the sponge Plakortis aff. simplex. Two new cyclic peroxide-containing polyketide C-16 acids 2 and 4 with their methyl esters 3 and 5 have been isolated from the Indo-Pacific marine sponge Plakortis aff. simplex. Both 2 and 3 are proposed to contain a single 1,2-dioxene ring, while...

Labombarda, F.; Barigou, A.; Legallois, D.; Campbell, K.; Bergot, E.; Milliez, P.; Saloux, E., 2016: Tricuspid systolic peak velocity is a prognostic factor in patients with cystic fibrosis. International Journal of Cardiology 199: 435-436

Falandysz, J., 1988: The occurrence and estimation of dietary intake of arsenic from Baltic fish in Poland. Mean arsenic content, mu g/kg fresh matter, in muscles of 784 Baltic fishes was: cod 920 (100 to 3900), herring 1200 (30 to 3200), sprat 2200 (100 to 7800), Platichthys flesus [flounder] 2100 (20 to 12 000), plaice 5100 (780 to 26 000), eel 330 (1...

Robin, G.C., 1961: Joplin's sling operation in adolescent hallux valgus. Journal of the American Podiatry Association 51: 260-266

Massari S.; Colonna R., 1986: Lipid miscibility and size increase of vesicles composed of two phosphatidylcholines. The size increase of small unilamellar vesicles composed of binary mixtures either of saturated fatty acid phosphatidylcholines with different chain lengths or of saturated and unsaturated phosphatidylcholines was found to depend on the miscibilit...

Beutler G., 1973: Relationships between the lower Kimmerian (Tertiary) structural geology and the deep basement. Veroeffentlichungen des Zentralinstituts fuer Physik der Erde 14, Teil 1, Stockwerkbau und Felderteilung): 113-118

Feinfeld, D.A., 1979: SLE--prognosis in focal nephritis. Hospital Practice 14(10): 21-21

Insull, W.; Hata, Y.; Meakin, J.D.; Marchant, L.; Andrews, C.W.; Buzek, B., 1974: Morphology of cholesteryl ester-rich inclusions in lesions of atherosclerosis in man. Atherosclerosis 19(3): 555-560

Wangensteen, O.H., 1961: Extended operations for alimentary tract malignancy: including the esophagus, stomach and colon, together with recital of experiences with the second look operation. Arizona Medicine 18: 319-328