+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Evidence that leukosialin, CD43, is intensely sulfated in the murine T lymphoma line RDM-4

, : Evidence that leukosialin, CD43, is intensely sulfated in the murine T lymphoma line RDM-4. Journal of Immunology 148(6): 1777-1783

Metabolic labeling of the murine T lymphoma cell line RDM-4 with [35S] sulfate results in intense incorporation into a cell-retained component of apparent Mr approximately 100,000. This macromolecule is identified as a glycoprotein by lectin chromatography. The sulfate is not incorporated as tyrosine sulfate. Release of the radiolabel by alkaline beta-elimination but not by endoglycosidase F is consistent with the sulfation of O- rather than N-linked oligosaccharides. The sulfated glycoprotein displays anomalous migration on SDS-PAGE in two respects: 1) the apparent Mr shifts from 115,000 to 87,000 on increasing the acrylamide concentration from 7 to 12%, and 2) on neuraminidase digestion migration is substantially reduced (apparent Mr 140,000). These properties indicate that the sulfated protein is both heavily glycosylated and extensively sialylated, and are characteristic of the lymphoid mucin, leukosialin (sialophorin, CD43). Specific labeling of the sialoglycoproteins of RDM-4 cells indicates that leukosialin, the most intensely labeled protein, comigrates with the sulfated protein on SDS-PAGE at varying acrylamide concentrations. Our data are therefore consistent with sulfation of at least some of the numerous O-linked oligosaccharides of this abundant glycoprotein in RDM-4 cells. No sulfation of CD43 in resting splenic T cells is observed.


PMID: 1541817

Other references

Hill J.H., 1977: Differential transmission of 3 soybean mosaic virus isolates by 2 aphid species. Proceedings Of The American Phytopathological Society:

Popeski, N.; Woodside, B., 2004: Central nitric oxide synthase inhibition disrupts maternal behavior in the rat. Blocking nitric oxide (NO) production, by 3rd ventricle administration of a nitric oxide synthase (NOS) inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME; 250 mug/5 mul, postpartum (pp)) decreased milk ejections in Day 10 pp rats. On Day 4 pp,...

Orlov, L.; Shevchenko, N.; Vuinyi, A., 1977: Chemical composition and calorie value of the carcasses of meat chickens receiving chemically synthesized L-lysine in their rations. Biulleten' Vsesoiuznogo nauchno issledovatel'skogo instituta fiziologii biokhimii i pitaniia sel'skokhoziaistvennykh zhivotnykh: ) 61-63

Reekmans, K.; D.V.cht, N.; Praet, J.; Fransen, E.; L.B.on, D.; Hoornaert, Cé.; Daans, J.; Goossens, H.; Van der Linden, A.; Berneman, Z.; Ponsaerts, P., 2015: Spatiotemporal evolution of early innate immune responses triggered by neural stem cell grafting. Stem Cell Research & Therapy 3(6): 56-56

Beauvais Anicet; Ritz Michel; Parisot Jean Claude; Bantsimba Christian, 2003: Testing etching hypothesis for the shaping of granite dome structures beneath lateritic weathering landsurfaces using ERT method. Granite domes, boulders and knobs buried within saprolite have been detected beneath lateritic weathering landsurfaces using 2D electrical resistivity tomography (ERT). This technique provides a valuable means of mapping the bedrock topography and...

Wackett, J., 2002: Factors affecting Yukon teen pregnancy decline in the mid and late 1990s. Teen pregnancy has declined throughout North America in the 1990s. In Yukon Territory, Canada, teen pregnancy in the late 1990s was almost 40% lower than in the early 1990s. This rate of decline is significantly greater than most recently reported...

Fruman, D.A.; Pai, S.Y.; Burakoff, S.J.; Bierer, B.E., 1995: A missense mutation in the calcineurin A-alpha gene expressed by EL4 lymphoma cells. 9TH INTERNATIONAL CONGRESS OF IMMUNOLOGY [Author] The 9th International Congress of Immunology : 14

Anonymous, 1994: Rural tourism and sustainable rural development. Proceedings of the Second International School on Rural Development, 28 June-9 July 1993, University College Galway, Ireland. This publication contains the proceedings of the Second International School on Rural Development, held in Galway, Irish Republic in 1993. The conference focused on the issues surrounding the development of rural tourism and of more sustainable fo...

Sun HuiZhong; H.X.eLi; Zhang YueJin; Chen TieShan, 2007: Study on microstructure features of seeds from 12 Seriphidium (Bess) Poljak. plants in China. In order to discuss the taxonomic characteristics of Seriphidium (Bess) Poljak. the seed microstructure features of its 12 species in China were observed under light microscope (LM) and scanning electron microscope (SEM). The results show that the...

Rattanapak, T.; Birchall, J.C.; Young, K.; Kubo, A.; Fujimori, S.; Ishii, M.; Hook, S., 2015: Dynamic visualization of dendritic cell-antigen interactions in the skin following transcutaneous immunization. Delivery of vaccines into the skin provides many advantages over traditional parenteral vaccination and is a promising approach due to the abundance of antigen presenting cells (APC) residing in the skin including Langerhans cells (LC) and dermal...