geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Extraction of type-IV collagenase/gelatinase from plasma membranes of human cancer cells


, : Extraction of type-IV collagenase/gelatinase from plasma membranes of human cancer cells. International Journal of Cancer 45(6): 1137-1142

Tumor proteinases are considered to be important in the process of cancer invasion and metastasis. We have proposed that the surface membrane localization of these proteinases places them in an optimal site to facilitate the invasion of surrounding extracellular matrix. In this study, we have used the organic solvent, n-butanol, and the detergent, n-octyl-glucoside, to sequentially extract metalloproteinases from crude plasma membranes of human RWP-I pancreatic cancer cells. Anion exchange chromatography and gel permeation chromatography were employed to further purify enzymes with the capacity to degrade gelatin, type-IV collagen, and carboxymethylated transferrin. Gelatin zymography was used to demonstrate proteinase bands of 92, 70 and 62-kDa. Immunoblotting of solubilized, partially purified pancreatic cancer plasma membrane proteins using polyclonal rabbit antibodies, which have specificity for type-IV collagenase/gelatinase, resulted in the recognition of a 70-kDa protein, but not the 92-kDa gelatinase. A type-IV collagenase/gelatinase of 68-kDa was similarly identified in A2058 human melanoma cancer cell plasma membranes.

US$29.90

PMID: 2161801


Other references

Spribille, Toby, 2002: The mountain forests of British Columbia and the American Northwest: Floristic patterns and syntaxonomy. An overview of the montane and subalpine forests of Abies lasiocarpa, Picea engelmannii, Abies amabilis and Tsuga mertensiana of northwestern North America is provided based on 541 releves of forest vegetation from coastal and inland British Colum...

Matsumoto T.; Morozumi Y.; Bando Y.; Hashimoto H.; Matsuoka A., 1981: Note on pravitoceras sigmoidale cretaceous heteromorph ammonite. P. sigmoidale Yabe (1902) was established on several fragmentary specimens. Based on better preserved specimens of subsequent collections, the characters of this peculiar heteromorph ammonite, are redefined. This species has an affinity with, and...

Volterrani, L.; Mazzei, M.Antonietta.; Fedi, M.; Scialpi, M., 2009: Computed tomography perfusion using first pass methods for lung nodule characterization: limits and implications in radiologic practice. Investigative Radiology 44(2): 124; Author Reply 124-124; Author Reply 124

Gupta, J.P.; Yadava, D.N., 1984: Bacteria in bile and their hazards in biliary tract surgery. Journal of the Indian Medical Association 82(10): 354-356

Vis, Aé.N.; Roemeling, S.; Kranse, R.; Schröder, F.H.; van der Kwast, T.H., 2006: Should we replace the Gleason score with the amount of high-grade prostate cancer?. Objectives: The stage and grade shift of currently diagnosed prostate cancer has led to a diminished prognostic power of the Gleason score system. We investigated the predictive value of the amount of high-grade cancer (Gleason growth patterns 4/...

Zhang, J-Jie.; Yang, J.; Li, Y-Chao.; Liu, N.; Zhang, R-Zhi., 2014: Genetic relationships of introduced Colorado potato beetle Leptinotarsa decemlineata populations in Xinjiang, China. The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), is an infamous invasive species worldwide that aggressively attacks potato and other Solanaceae crops. CPB was first found in China in 1993 and has since spread across 2.77 × 10(5...

Bergeron, G.A., 1958: Physiological development of male & female adolescents. Laval Medical 25(3): 348-374

Colombie Claude; Rameil N., 2007: Tethyan to Boreal correlation in the Kimmeridgian using high-resolution sequence stratigraphy (Vocontian Basin, Swiss Jura, Boulonnais, Dorset). Geologische Rundschau = International Journal of Earth Sciences (1999) 96(3): 567-591

Arnal, E.; Debrot, E.; Marcano, R.; Montagne, A., 1993: Population fluctuation of whiteflies and its relation with tomato yellow mosaic at a Venezuelan locality. A study of populations of adult whiteflies captured on four columnar sticky yellow traps, placed at the four cardinal points around experimental plots of tomatoes, was related to the incidence of tomato yellow mosaic (TYM), a virus disease respons...

Frezzi, M.J., 1965: Leaf scorch caused by L. arachidicola and other fungi in wild peanuts (rhizomatous group) of different origin. Hitherto exclusively attacking Arachis burkartii, A. glabrata and A. hagenbeckii plants originating from north-east Argentina, Paraguay and the southern Mato Grosso (Brazil), L. arachidicola has new been found to infect cultivated varieties. The w...