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Growth-associated gene expression is not constant in cells traversing G-1 after exiting mitosis

, : Growth-associated gene expression is not constant in cells traversing G-1 after exiting mitosis. Journal of Cellular Physiology 147(2): 231-241

Analysis of gene expression following stimulation of growth-arrested cells has been the main approach for identification of growth-associated genes. Since the activation of these gene sequences is dependent on both the stimulatory agent and the state of quiescence of the cell, the activation and role of the same genes may be entirely different in non-growth arrested, actively proliferating cells. We have addressed the question of growth-associated gene expression during active growth by analyzing gene expression during G-1 of cells which have just exited mitosis without first leaving the cell cycle. We were able to isolate, by a non-inductive, drug free system, a population of highly synchronized Swiss 3T3 cells within mitosis (greater than 90%) in numbers sufficient to determine the pattern of expression of a large number of representative growth-associated genes. Our results show that after replating the mitotic cells into conditioned medium: (1) growth-associated gene expression is not constant during G-1 of actively proliferating cells, and (2) while a number of genes (e.g., JE, c-myc, ODC, p53, and histone) exhibited patterns of expression similar to that reported in the quiescent systems, others (e.g., nur-77, vimentin, calcyclin) exhibited patterns which were completely different. From these results, we can begin to construct a temporal map of G-1 progression during active growth.


PMID: 2040657

DOI: 10.1002/jcp.1041470207

Other references

Pytskii, V.I., 1957: A patho-physiological mechanism of immunity following dysentery intoxication in rabbits. Rabbits immunized with a heated vaccine of the Flexner type S, strain 1 or 338, were challenged with a lethal dose of complete antigen. The intoxication was mild and the blood circulation rate somewhat lowered, indicating an arteriolar spasm in th...

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Maurer, J.J.; Doggett, T.A.; Burns Keliher, L.; Curtiss, R., IIi, 2000: Expression of the rfa, LPS biosynthesis promoter in Salmonella typhimurium during invasion of intestinal epithelial cells. Salmonella regulates transcription of many of its genes in response to environmental conditions encountered inside or outside the eukaryotic cells it infects. In this paper, we examined Salmonella typhimurium gene expression within epithelial cell...

Monden Y.; Uyama T.; Kimura S.; Taniki T., 1991: Extrathymic malignancy in patients with myasthenia gravis. The presence of extrathymic malignancies was investigated in 296 thymectomised myasthenia gravis (MG) cases. In 5 of the 296 cases, extrathymic malignant tumours were observed. 4 of the 5 cases had thymomatous MG. 3 cases had malignant fibrous his...

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Anonymous, 2014: Etymologia: Sarcocystis nesbitti. Emerging Infectious Diseases 19(12): 1974-1974

Frisch-Niggemeyer, W., 1956: Absolute amount of ribonucleic acid in viruses. Nature 178(4528): 307-308

Harr, D.P.; Knight, L.C.; Charkes, N.C.; Urbain, J.L.; Krevsky, B.; Fisher, R.S.; Maurer, A.H., 1993: Can liquids by used to predict abnormal solid phase gastric emptying?. Journal of Nuclear Medicine 34(5 SUPPL ): 11P

Hoppner, E., 1958: New varieties of potato in 1958. The following varieties, in addition to the export variety Fecula, were recognized in 1958: very early, Imme; early, Gallo; medium-early, Andrea, Climax and Feldeslohn (culinary), Petra (culinary and industrial); medium-late to late, Servena and I...

Draghici, L.; Bude, A., 1974: The quality of barley for malting and for fodder from the aspect of genetics and methods of breeding. In this review of research, chemical composition, inheritance of quality characters and methods of breeding to improve both malting and forage quality are discussed. Quality assessment carried out on various varieties and lines at Fundulea and els...