+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Guanine nucleotide regulation of [3H]vasopressin binding to liver plasma membranes and solubilized receptors. Evidence for the involvement of a guanine nucleotide regulatory protein

, : Guanine nucleotide regulation of [3H]vasopressin binding to liver plasma membranes and solubilized receptors. Evidence for the involvement of a guanine nucleotide regulatory protein. Biochemical Journal 240(2): 361-365

A guanine nucleotide regulatory protein may be involved in vasopressin-receptor-mediated polyphosphoinositide breakdown in rat liver. Therefore we examined the effects of the non-hydrolysable guanine nucleotide guanosine 5'-[beta gamma-imido]triphosphate (p[NH]ppG) on [3H]vasopressin ([3H]AVP) binding to hepatic plasma membranes and detergent extracts. [3H]AVP bound to a single set of high-affinity binding sites in membranes. Addition of p[NH]ppG decreased the affinity of receptor binding without altering the maximal binding capacity. The rate of dissociation of [3H]AVP from membrane-bound receptors was also enhanced by p[NH]ppG. Solubilization of [3H]AVP-prelabelled membranes with dodecyl beta-D-maltoside resulted in a [3H]AVP-receptor complex that was unstable in solution. Incubation of these extracts for 5 min at 30 degrees C resulted in a 40% loss of bound [3H]AVP, whereas in the presence of p[NH]ppG there was a 54% loss. However, when membranes were prelabelled with [3H]AVP and p[NH]ppG and then solubilized, the resulting hormone-receptor complex was still temperature-labile but insensitive to the further addition of p[NH]ppG. The molecular size of soluble vasopressin receptors was estimated by gel filtration. The [3H]AVP-receptor complex was eluted as a single peak with an apparent molecular size of 258 kDa. However, no peak was detected when solubilized extract was made from membranes prelabelled with [3H]AVP and p[NH]ppG, suggesting that this receptor complex had dissociated during chromatography. It is possible therefore that the high-Mr complex contains the hormone, its receptor and a guanine nucleotide binding protein.


PMID: 2949738

Other references

Lightowlers, R.; Takamiya, S.; Wessling, R.; Lindorfer, M.; Capaldi, R.A., 1989: Cloning and sequencing of the cDNA for a 13th different subunit (IHQ) of beef heart cytochrome c oxidase. A cDNA encoding the one remaining uncharacterised subunit, subunit IHQ, was isolated. The cDNA was 480 bp long, and encoded a polypeptide which was either 83 or 88 amino acids long, including an N-terminal leader sequence of either 27 or 32 residu...

Baskina V.A., 1974: Igneous processes associated with ore-bearing zones and large ore junctions. Pages 165-172 1974

Rabor, Di0sc0r0 S., 1938: Birds from Leyte. Collections and observations were carried on in the vicinity of Helosig, a highland barrio in the central highlands of the southern third of Leyte, in May, 1937. 257 specimens, belonging to 77 forms, were collected. 17 forms are newly recorded fro...

Ribeiro, M.E.; Elliot, J.S., 1964: Direct Enzymatic Determination Of Urinary Oxalate. Urinary oxalate may be determined directly by means of an oxalic acid decarboxylase after preliminary ultrafiltration and concentration. The method is superior in terms of accuracy and time involved to ether extraction methods.

Seager, SWJ.; Demorest, CN., 1978: Carnivores (Carnivora). Reproduction of captive wild carnivores. Unknown

Pesce, C.; Morrow, M., 2013: The need for lymph node dissection in nonmetastatic breast cancer. Determining whether cancer has spread to locoregional lymph nodes is a critical step in the initial staging of breast cancer patients. Although axillary dissection reliably identifies nodal metastases and prevents the recurrence of cancer in the a...

Jaeger, J.A.; SantaLucia, J.; Tinoco, I., 1993: Determination of RNA structure and thermodynamics. We have developed a new computer program for tissue segmentation designed for the quantification of smooth muscle in the corpus cavernosum. The program uses digitalized images of stained histological sections taken with a CCD camera. The recogniti...

Baskin, L.S.; Hayward, S.W.; Sutherland, R.A.; Disandro, M.J.; Thomson, A.A.; Goodman, J.; Cunha, G.R., 1996: Mesenchymal-epithelial interactions in the bladder. During bladder development, undifferentiated mesenchymal and epithelial cells undergo an orderly sequence of differentiation defined by the expression of smooth-muscle (alpha-actin, myosin, vinculin, desmin, vimentin, and laminin) and epithelial (...

Kinzer, D.M., 1956: Hospitals give Chicago a shot in the arm. Modern Hospital 87(5): 61-64

Shiple, B.J., 1997: Relieving low-back pain with exercise. If you suffer from low-back pain, the most important part of your treatment is something only you can give yourself: exercise. Regular workouts make pain go away faster. By strengthening the muscles that support your back and improving your back&a...