+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

High molecular mass forms of basic fibroblast growth factor are initiated by alternative CUG codons

, : High molecular mass forms of basic fibroblast growth factor are initiated by alternative CUG codons. Proceedings of the National Academy of Sciences of the United States of America 86(6): 1836-1840

A 6.75-kilobase human hepatoma-derived basic fibroblast growth factor (bFGF) cDNA was cloned and sequenced. An amino-terminal sequence generated from a purified hepatoma bFGF was found to correspond to the nucleotide sequence and to begin 8 amino acids upstream from the putative methionine start codon thought to initiate a 154-amino acid bFGF translation product. This sequence suggests that a form of bFGF of at least 163 amino acids exists. The hepatoma cDNA was transcribed in vitro into RNA; in vitro translation of this RNA generated three forms of bFGF with molecular masses of 18, 21, and 22.5 kDa. By use of in vitro mutagenesis, it was found that the 22.5-kDa bFGF and possibly the 21-kDa form were initiated with CUG start codons. The 18-kDa bFGF was initiated with an AUG codon. By transfecting into COS cells human hepatoma bFGF cDNA and a construct from which the AUG initiator was eliminated, it was found that the higher molecular mass forms of bFGF were as biologically active as the 18-kDa form.


PMID: 2538817

Other references

Schonberg, I.L., 2016: The spreading factors in eczematous processes. Ohio State Medical Journal 43(12): 1240-1242

Onderoğlu, L.S., 1998: Digital examination and transperineal ultrasonographic measurement of cervical length to assess risk of preterm delivery. To compare the diagnostic performance of cervical length measurement determined with transperineal ultrasonography and digital assessment of the cervix for the prediction of preterm delivery in patients with preterm labor. 90 patients admitted to...

Durkee, J.W.; Antich, P.P.; Lee, C.E., 1990: Exact solutions to the multiregion time-dependent bioheat equation. II: Numerical evaluation of the solutions. Analytic solutions to the time-dependent multiregion bioheat equation are evaluated numerically for cartesian and spherical models. Principle and alternate branch contributions to the solutions are examined. The calculations include a parametric i...

Hershey, L.A., 1996: Dementia: not always a disease of the elderly. Dural arteriovenous fistula is an uncommon cause of myelopathy. The nidus of the malformation is on or within the dura and results in a mass of serpentine vessels on the dorsal surface of the spinal cord, usually at low thoracic or lumbar levels....

Satz, J.; Sandt de Villiers JF.V.A.; Abrahams, L.C., 1970: Editorial. The Journal and its Editor. Journal of the Dental Association of South Africa 25(10): 325-326

Gyer, Jo, 1974: Rescue: the aim is rescue, not just removal. Green scene: 3 (1) 2-7

Liu, B.; Schaider, L.A.; Mason, R.P.; Bank, M.S.; Rabalais, N.N.; Swarzenski, P.W.; Shine, J.P.; Hollweg, T.; Senn, D.B., 2009: Disturbance impacts on mercury dynamics in northern Gulf of Mexico sediments. Journal of Geophysical Research 114.G

Black, J.A.; Chan, G.F.; Hew, C.L.; Dixon, G.H., 1970: Gene action in the human haptoglobins. 3. Isolation of the alpha-chains as single gene products. Isolation, molecular weight, and amino acid composition of alpha and beta chains. Canadian Journal of Biochemistry 48(1): 123-132

Vit, S., 1981: Une nouvelle espece du genre Tohlezkus Vit de Taiwan (Coleoptera, Eucinetidae). A new species Tohlezkus orientalis is described for the genus Tohlezkus errected by Vit (1977b) for the apterous species T. ponticus from northeastern Turkey. Only 2 winged species of the genus Eucinetus Germar are known from the Oriental region.

Wrage, Lj, 1987: Wild proso, woolly cupgrass. Field facts: soils insects diseases weeds crops South Dakota State University Cooperative Extension Plant Science Department987 2(19): 3