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Human-parathormone assay for use in dogs: validation, sample handling studies, and parathyroid function testing

, : Human-parathormone assay for use in dogs: validation, sample handling studies, and parathyroid function testing. American Journal of Veterinary Research 50(7): 1123-1127

Ten commercially available parathormone (PTH) assays were competitively validated, using dilutional parallelism, intra-assay and interassay coefficients of variation, and sensitivity and measured responses of 2 dogs to calcium and EDTA infusions. A 2-site immunoradiometric assay for intact human-PTH was superior to the others for estimating canine-PTH, met the criteria for validity, and was further investigated. A series of sample-handling studies was performed. Serum and plasma samples stored at 24 C lost 15% (n = 5; P less than 0.05) of PTH between 2 and 24 hours. This did not occur at 6 C. The mean PTH concentration of sera from blood samples clotted at 24 C was 6% (P less than 0.05) higher than equivalent EDTA samples. Serum samples stored at 6 and 37 C deteriorated 35% and 100% (n = 5; P less than 0.05), respectively, after 1 week, whereas samples stored at -20 and -70 C for 4 weeks did not deteriorate. There was no significant deterioration of PTH in samples frozen (-40 C) and thawed up to 7 times (n = 5). Parathyroid function testing was investigated by use of 2-hour infusions of disodium EDTA (25 mg/kg/h), 10-minute infusions of calcium gluconate (3 mg of elemental calcium/kg/10 min), and physiologic saline controls (n = 8). Renal function was monitored before and after EDTA infusion by exogenous creatinine clearance. Infusion of disodium EDTA increased mean PTH concentration from 67 (time 0) to 317 and 235 pg/ml at 90 and 180 minutes, respectively (P less than 0.001). Infusion of calcium gluconate decreased mean PTH concentration from 84 (time 0) to 14 and 12 pg/ml at 15 and 60 minutes, respectively (P less than 0.005). There were no observable side effects of the infusions in normal conscious dogs and no differences in exogenous creatinine clearance after EDTA infusion.


PMID: 2505649

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Pearce, C.Martin.; de Lusignan, S.; Phillips, C.; Hall, S.; Travaglia, J., 2013: The computerized medical record as a tool for clinical governance in Australian primary care. Computerized medical records (CMR) are used in most Australian general practices. Although CMRs have the capacity to amalgamate and provide data to the clinician about their standard of care, there is little research on the way in which they may b...

Yamaguchi M.; Kitade M.; Okada S., 1979: Decrease in calcium content in the femur of rats orally administered stannous chloride. The Ca content in the femur of rats was significantly decreased by the oral administration of stannous chloride (3.0 mg Sn2+/100 g body wt, 6 .times. at 12 h intervals). This decrease was dose-dependent. Administration of Sn did not prevent femora...

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Radionov, B.V.; Kogosov, I.A.; Konovalov, E.P.; Khmel', O.V.; Gumeniuk, N.I., 1991: Clinical and experimental studies of the effect of low-intensity laser irradiation on the blood and blood vessels. Sovetskaia Meditsina: 27-29

Belicza, M.; Cupic, H.; Kruslin, B.; Simunovic, D.; Lenicek, T.; Gladic, V.; Lechpammer, M.; Kusic, Z., 2000: Possible effects of Chernobyl accident and war aggression on Croatia on thyroid and breast cancer epidemiology. Pathology International 50(Supplement): A78, October

Vasil' eva, R.A.; Tsyrenov, V.Z.; Zhdanova, E.A., 1977: Excretion of nucleotides in lactic acid bacteria. 44 strains of Streptococcus lactis, 10 of Str. cremoris, 36 of Str. diacetilactis, 8 of Str. diacetilactis subsp. acetoinicus, and 9 of Str. paracitrovorus [Leuconostoc dextranicum] from the collections of the All Union Butter and Cheese Industry...

Baschinsky, D.; Hameed, A.; Keyhani Rofagha, S., 1999: Fine-needle aspiration cytological features of dermoid cyst of the parotid gland: A report of two cases. We describe the cytological features of dermoid cyst of the parotid gland and the value of preoperative diagnosis by fine-needle aspiration (FNA) cytological evaluation. Both patients had painless parotid masses. On physical examination, a freely...

Laihinen, A.; Rinne, J.O.; Rinne, U.K.; Haaparanta, M.; Ruotsalainen, U.; Bergman, J.; Solin, O., 1992: Fluorine 18 6 fluorodopa pet scanning in parkinson's disease after selective comt inhibition with nitecapone or 462. PET studies were performed to investigate the effects of a new catechol-O-methyltransferase (COMT) inhibitor, nitecapone (OR-462 [3-(3,4-dihydroxy-5-nitrobenzylidene)-2,4-pentadione]), on the accumulation of dopamine in the striatum and whether it...

Tencati, J.R.; Novey, H.S., 1983: Hypersensitivity angiitis caused by fumes from heat-activated photocopy paper. A 53-year-old librarian had recurrent palpable purpura on her ankles and legs that was found to be caused by the fumes released from heat-activated photocopy paper at her place of employment. Behenic acid was identified as the responsible chemical...

Gil Salom, M.; Espi Macias, A.; Chuan Nuez, P.; Arenas Ricart, J.; Clar Blanch, F.; Carretero González, P.; García-Lomas, J., 1988: Serum immunosuppressive activity of patients undergoing urologic surgery. Actas Urologicas Espanolas 12(6): 544-548