geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and characterization of mutations responsible for a runaway replication phenotype of plasmid R1


, : Identification and characterization of mutations responsible for a runaway replication phenotype of plasmid R1. Gene 57(2-3): 203-211

Initiation of replication of the resistance plasmid R1 is carefully regulated by the two negatively acting factors, CopA and CopB. It is shown here that the temperature-dependent runaway-replication phenotype of an R1 plasmid mutant is caused by two point mutations in each of the promoters for the genes of these control factors. Expression of the two genes is affected in the following way: (1) one C-to-T transition in the putative -35 box of the copB-repA operon creates a two- to three-fold stronger promoter from which expression is temperature-dependent; (2) another C-to-T transition in a G + C-rich area immediately downstream from the -10 box of the copA promoter reduces expression of the copA gene three-fold. The phenotypic consequences of the two mutations are discussed in the light of the current model for R1 replication control.

US$29.90

PMID: 3692168


Other references

Santagiustina, M.; Wright, E.M., 1995: Suppression of third-order dispersion radiation in solid-state soliton lasers. Optics Letters 20(22): 2267-2267

Ghazal, T.S.; Levy, S.M.; Childers, N.K.; Broffitt, B.A.; Caplan, D.J.; Warren, J.J.; Cavanaugh, J.E.; Kolker, J., 2016: Dental Caries in High-risk, School-age African American Children in Alabama: A Six-year Prospective. The purpose of this study was to assess the prevalence and incidence of dental caries in school-age African American children who received semi-annual fluoride varnish applications. A cohort of six-year-old high caries-risk African American childr...

Taylor, L.E.; Maynard, M.A.; Friedmann, P.D.; Macleod, C.J.; Rich, J.D.; Flanigan, T.P.; Sylvestre, D.L., 2013: Buprenorphine for human immunodeficiency virus/hepatitis C virus-coinfected patients: does it serve as a bridge to hepatitis C virus therapy?. Buprenorphine is associated with enhanced human immunodeficiency virus (HIV) treatment outcomes including increased antiretroviral therapy initiation rates, adherence, and CD4 cell counts among HIV-infected opioid-dependent individuals. Buprenorph...

Hauser, S., 1990: Water and nutrient dynamics under alley cropping versus monocropping in the humid-subhumid transition zone. Investigations were carried out in 1988 and 1989 on a degraded alfisol with a slope of 5-7% at Ibadan, Nigeria, in an established alley cropping trial with Leucaena leucocephala as hedgerow trees planted perpendicular to the slope and at 4-m inter...

Van Liew, H.D.; Mahajan, R.K., 1989: Display of the alveolar plateau of single-breath tests in "dilution index" format. In 1949, Fowler (J. Appl. Physiol. 2: 283-299) advocated calculation of a "dilution index" from data of the alveolar plateau of single-breath tests; the calculation provides an estimate of the dilution of resident gas in the lung that ga...

Joshi, M.M., 1973: Specific microbial food chains and soil classification. Nematodes are involved in many specific and interlocking food chains and the species differ between soil types. There is thus some basis for using nematodes and other soil organisms for biological classifications of soil.

O'Dea, K., 1999: The prevalence of pressure damage in acute care hospital patients in the UK. This paper aims to provide information on the prevalence of pressure damage in UK hospital patients since 1992. A survey method and data handling service provided by a medical device company was used to set targets and monitor trends. The results...

Biehn, T.L.; Elhai, J.D.; Fine, T.H.; Seligman, L.D.; Richardson, J.Don., 2012: PTSD factor structure differences between veterans with and without a PTSD diagnosis. This study examined differences in posttraumatic stress disorder's (PTSD) factor structure between veterans with and without a PTSD diagnosis. An archival dataset of 378 trauma-exposed Canadian veterans (mostly men) was used. The sample was d...

Dahme, E.; Bilzer, T.; Matic, G.; Hafner, A.; Kaiser, E., 1987: Immunohistochemical study of canine tumours of the cranial and spinal nerve roots. An immunohistochemical evaluation of the cell markers S 100, GFAP, Leu 7, NSE, Desmin and Cytokeratin, was made on a canine tumour collection to improve the classification and grading of tumours in nerve roots. A differentiation between neurinomas...

Parton P.; N.W.G.; Shaw K.N.F.; Giudici T.A.; Scher E.; Carter M.L.; Kumor E.; Falk R.; Donnell G.N.; L.T.; Sweetman L.S., 1986: Complications in diagnosis of multiple carboxylase deficiency. Clinical Research 34(1): 121A