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Identification and intracellular localization of the unc-22 gene product of Caenorhabditis elegans

, : Identification and intracellular localization of the unc-22 gene product of Caenorhabditis elegans. Genes & Development 2(1): 93-105

The unc-22 gene is one of a set of genes identified using classical genetics that affect muscle structure and function in the free-living nematode Caenorhabditis elegans. Since cloning the unc-22 gene by transposon tagging, we have used conventional techniques combined with a set of Tc1 transposon insertion alleles to characterize the gene and its products. The gene extends over more than 20 kb of genomic sequence and produces a transcript of approximately 14 kb. A polyclonal antibody raised against an Escherichia coli beta-galactosidase-unc-22 fusion protein recognizes a polypeptide in nematode extracts that is between 500,000 and 600,000 daltons and labels the muscle A-band in indirect immunofluorescent microscopy. The Tc1-induced alleles have been used at every stage to verify these conclusions. The Tc1 insertions are spread over much of the region that contributes to the mature transcript; in most alleles, Tc1 sequences are incorporated into a composite unc-22-Tc1 transcript. The large protein is either absent or severely reduced in amounts in the mutants. In one case, a truncated polypeptide was also identified. The location of the protein in the A-band, along with earlier genetic data, suggests that the unc-22 product may interact with myosin to regulate its function.


PMID: 2833427

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Davidson, R.Andrew.; Sugiyama, C.; Guo, T., 2014: Determination of absolute quantum efficiency of X-ray nano phosphors by thin film photovoltaic cells. The absolute optical power at 611 nm emitting from Eu doped Gd2O3 nano phosphors upon X-ray excitation from a microfocus X-ray source operated at 100 kV was measured with thin film photovoltaic cells (TFPCs), whose optical response was calibrated...

Pick, E., 1986: Microassays for superoxide and hydrogen peroxide production and nitroblue tetrazolium reduction using an enzyme immunoassay microplate reader. Methods in Enzymology 132: 407-421

E.S.da, M.; Bergère, J.L.; Desmazeaud, M.J., 1978: Detection and localization of peptide hydrolases in Lactobacillus casei. The proteolytic system of L. casei NCDO 151 is described. It possesses specific activities of aminopeptidase, dipeptidase, carboxypeptidase, endopeptidase and caseinolytic activity. All of these enzymes are located in the cell cytoplasm.

Moscona, A., 1957: The Development In Vitro Of Chimeric Aggregates Of Dissociated Embryonic Chick And Mouse Cells. Dissociated cells from various organ rudiments of chick and mouse embryos when intermixed in suspension cultures, readily aggregated and combined to form composite, chimeric tissues. Under suitable conditions of culture, such reconstituted tissues...

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Ohshima, Y.; Ohno, Y.; Kajiyama, K.; Takahashi, K., 1986: High-performance liquid chomatographic separation of rhubarb constituents. An HPLC procedure using a gradient solvent system was developed for the simultaneous separation from a chloroform/methanol extract of rhubarb [Rheum officinale] of monomeric anthraquinones, their glycosides and dimeric anthrone glycosides; and 3-d...

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Blazar, B.R.; Widmer, M.B.; Taylor, P.A.; Vallera, D.A., 1992: Promotion of murine marrow alloengraftment and hematopoietic recovery across the major histocompatibility barrier by administration of recombinant human interleukin-1 alpha. Irradiated C57BL/6 (H-2b) recipients of T-cell-depleted (TCD) BALB/c (H-2d) bone marrow (BM) and recombinant interleukin-1 alpha (IL-1 alpha) (1 microgram/d) had a significantly (P less than or equal to .006) higher 100-day actuarial survival rate...

Maloletnev, V.I.; Moyanova, S.G., 1978: Effect of thalamic relay nuclei damage on the structure of sleep in cats. In chronic experiments on cats shortly after local electrical coagulation in the thalamic relay nuclei (ventro-postero-lateralis and lateral geniculate body) drastic qualitative and quantitative changes were observed in the sleep structure. The ch...