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Identification by immunoblotting of three halothane-induced liver microsomal polypeptide antigens recognized by antibodies in sera from patients with halothane-associated hepatitis


, : Identification by immunoblotting of three halothane-induced liver microsomal polypeptide antigens recognized by antibodies in sera from patients with halothane-associated hepatitis. Journal of Pharmacology and Experimental Therapeutics 242(2): 733-740

Previous studies have demonstrated that sera from patients with severe liver damage after halothane anesthesia ("halothane hepatitis") contain antibodies reacting with novel antigenic determinants expressed on hepatocytes from rabbits exposed previously to halothane. To determine the structure of the halothane-induced antigen(s), immunoblotting experiments were performed using patient sera and rabbit liver subcellular fractions. Three polypeptide antigens (Mr 100,000, 76,000 and 57,000) expressed in liver fractions from animals sacrificed 16 hr after exposure to 1% halothane in oxygen for 45 min, but not in fractions from unexposed animals, were identified. Analysis of fractions prepared by differential and sucrose density gradient centrifugation, and characterized by enzyme marker analysis, localized all three antigens to a microsomal subfraction relatively enriched in glucose-6-phosphatase activity, therefore, presumably derived from the endoplasmic reticulum. Antibodies to these antigens were detected in 19 of 24 sera from patients with halothane hepatitis, and four distinct patterns of antibody specificity were observed: 100,000 + 76,000 (seven patients), 100,000 alone (seven patients), 76,000 alone (three patients) and 57,000 alone (two patients). Such antibodies were not detectable in sera from 24 normal blood donors or 36 control patients. Thus, halothane induces expression of three distinct polypeptide antigens in liver, and patients with halothane hepatitis differ in patterns of recognition of these antigens by circulating antibodies.

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PMID: 3302210


Other references

Ageel, A.R.; Amin, M.A., 1998: Integration of schistosomiasis-control activities into the primary-health-care system in the Gizan region, Saudi Arabia. A few decades ago, 43-91% of people living in the Gizan region of Saudi Arabia were infected with Schistosoma haematobium, the dominant schistosome in the region and one which is endemic in several provinces. However, a vertical disease control pr...

Eckmann, L.; Stenson, W.F.; Savidge, T.C.; Lowe, D.C.; Barrett, K.E.; Fierer, J.; Smith, J.R.; Kagnoff, M.F., 1997: Role of intestinal epithelial cells in the host secretory response to infection by invasive bacteria. Bacterial entry induces epithelial prostaglandin h synthase-2 expression and prostaglandin E2 and F2alpha production. Increased intestinal fluid secretion is a protective host response after enteric infection with invasive bacteria that is initiated within hours after infection, and is mediated by prostaglandin H synthase (PGHS) products in animal models of infec...

Shimada, I., 1995: An analysis of decision-making process in help-seeking behavior. The purpose of this study was to analyze decision-making process in help-seeking behavior with the information monitoring method. Four simulated situations were prepared in which help became necessary. Eight categories of further situational infor...

Turner, M.S.; Helmann, J.D., 2000: Mutations in multidrug efflux homologs, sugar isomerases, and antimicrobial biosynthesis genes differentially elevate activity of the sigma(X) and sigma(W) factors in Bacillus subtilis. The sigma(X) and sigma(W) extracytoplasmic function sigma factors regulate more than 40 genes in Bacillus subtilis. sigma(W) activates genes which function in detoxification and the production of antimicrobial compounds, while sigma(X) activates f...

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Yao, R.; Doig, P.; Burr, D.H.; Trust, T.J.; Guerry, P., 1996: Isolation and characterization of a gene required for pilus biosynthesis in Campylobacter jejuni. Abstracts of the General Meeting of the American Society for Microbiology 96(0): 166

Gómez-Blanco, J.Carlos.; Martínez-Reina, F.Javier.; Cruz, D.; Pagador, J.Blas.; Sánchez-Margallo, F.M.; Soria, F., 2016: Fluid Structural Analysis of Urine Flow in a Stented Ureter. Many urologists are currently studying new designs of ureteral stents to improve the quality of their operations and the subsequent recovery of the patient. In order to help during this design process, many computational models have been developed...

Markin, G.P.; Silva, L.; Aguiar, A.M.F., 1995: The insect fauna associated with the tree Myrica faya (Myricaceae) in the Macaronesia Islands and on Mainland Portugal. Myrica faya AITON, a plant native to the Macaronesia Islands, has been accidentally introduced to Hawaii where it has become a serious pest of our forests and other native ecosystems. Since the 1950's, an intensive survey of the insects assoc...

Segizabeva, M.O.; Isaev, G.G., 1995: The control of breathing under conditions of altered density of the gas Milieu during exercise. Pfluegers Archiv European Journal of Physiology 430(4 SUPPL ): R159

Soares, J., 1999: Critical commentary to occult papillary carcinoma of the thyroid gland with extensive osseous metastases A case report with review of the literature. Pathology Research & Practice 195(10): 715-716