geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of Plasmodium falciparum-infected mosquitoes using a probe containing repetitive DNA


, : Identification of Plasmodium falciparum-infected mosquitoes using a probe containing repetitive DNA. Molecular and Biochemical Parasitology 32(2-3): 105-112

A cloned repetitive DNA sequence (rep20) was evaluated as a diagnostic probe specific for Plasmodium falciparum sporozoites using experimentally infected mosquitoes squashed directly on nylon filters. Head/thorax portions of mosquitoes, killed 14-16 days after ingesting P. falciparum-infected blood, gave positive signals when examined for the presence of P. falciparum sporozoite DNA by hybridisation. This correlated with the number of oocysts found in a sample of the same batch of mosquitoes examined by dissection. No positive signals were obtained with 50 Plasmodium berghei-infected mosquitoes probed with the rep20 sequence. The results indicate that a probe containing rep20 may be useful in the rapid and specific incrimination of vectors carrying P. falciparum sporozoites. The value of repetitive DNA in the diagnosis of malaria is discussed.

US$29.90

PMID: 2648141


Other references

Trebous, B., 1975: Some data on the fertilization of potato crops. Pomme de terre francaise: 37 (370) 19-21

Higa, T., 1991: Bioactive phenolics and related compounds. Bioorganic Marine Chemistry, 4: 33-90

Fain, A.; Lukoschus, F.S., 1979: Parasites of Western Australia. IX. Myobiidae parasitic on rodents (Acarina: Prostigmata). Six new species of Myobiidae are described from specimens collected from rodents in Western Australia.

Stadtmann, H., 2001: Dose quantities in radiation protection and dosemeter calibration. Dosimetric concepts and the definition of dose quantities for use in radiation protection were defined by the ICRP and the ICRU. Three types of quantities are of relevance for radiation protection purposes: basic physical quantities, protection qu...

Niu, Z-ru.; Yue, X-jing.; Kong, Q-yu.; Wang, Y-fen.; Yao, Y-qing., 2014: A meta-analysis of preventing bone mineral loss in patients with endometriosis treated by gonadotrophin-releasing hormone analogues with add-back therapy. To evaluate the role and efficacy of preventing bone mineral loss in patients with endometriosis treated by gonadotrophin-releasing hormone analogues (GnRH-a) combined with add-back therapy. Prospective, randomized controlled studies of the use of...

Chappelow, A.V.; McMahon, J.T.; Jones, C.L.; Kosmorsky, G., 2010: Granulomatous orbital inflammation associated with intraorbital titanium-impregnated acetate fiber. Herein we report a 52-year-old man with subacute right-sided proptosis and diffuse intraconal enhancing abnormality on MRI. Orbital biopsy revealed granulomatous inflammation consistent with idiopathic orbital inflammatory syndrome (IOIS), or orbi...

Taira, T.; Hori, T., 2007: Diaphragm pacing with a spinal cord stimulator: current state and future directions. Diaphragm pacing with electrical stimulation of the phrenic nerve is an established treatment for central hypoventilation syndrome. The device, however, is not readily available. We tested the same spinal cord stimulator we use for pain control in...

Whimster, IW., 1980: Neural induction of epidermal sensory organs in gecko skin. Linnean Society Symposium Series, 161-167 No. 9

Ruozi, B.; Tosi, G.; Forni, F.; Angela Vandelli, M., 2006: Ketorolac tromethamine liposomes: encapsulation and release studies. Liposomes loaded with ketorolac tromethamine salt were prepared by using a thin layer evaporation method. The physical properties of liposomes were studied by using atomic force microscopy (AFM) and transmission electron microscopy (TEM). The rela...

Najda, J.; Gmiński, J., 1992: Silica in pathophysiology of the central nervous system. Polski Tygodnik Lekarski 47(20-21): 459-460