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Identification of calcium binding proteins in two-dimensional gel electrophoretic pattern of Drosophila thorax and their distribution in two types of muscles


, : Identification of calcium binding proteins in two-dimensional gel electrophoretic pattern of Drosophila thorax and their distribution in two types of muscles. Journal of Biochemistry 104(4): 489-491

Ten Drosophila thorax proteins (six myosin light chains and four proteins called a, b, c, d) were found to have high affinities with Ca2+. This was proved after subjecting the total Drosophila thorax proteins to two-dimensional (2D) transblot, followed by 45Ca2+ autoradiography. Three proteins (a, c, d) showed Ca2+ dependent electrophoretic mobility changes. To know their tissue-specific localization, fibrillar and tubular type muscle fibers were individually dissected from freeze-dried flies and separately subjected to 2D gel electrophoresis. Fibrillar type muscle had protein b and a small amount of protein a. Tubular type muscle had proteins c, d and a very large amount of protein a. Protein d was characterized to be calmodulin.

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PMID: 3240990


Other references

Krell, J.; Stebbing, J., 2012: Circulating tumour cells as biomarkers in early breast cancer. Lancet. Oncology 13(7): 653-654

Pont,S.J.; Huizinga,M.M.; Fields,W.T.; Beech,B.M., 2008: Targeting teeth: pediatric obesity and the dental office. Obesity Management: 3, 104-110

Sadykov S.S., 1990: Increasing tolerance to verticillium wilt in cotton cultivars. Uzbekskii Biologicheskii Zhurnal (1): 53-56

Etzioni, A., 1974: On incrementing a hurricane. Science 186(4166): 783-783

Parfent'-Eva, T.; Chernenko, E.; Isaeva, N.; Iatsenko, T., 1975: Influence of precipitant excess on the completion of precipitation of potassium lime from sodium eluate. Izvestiia vysshikh uchebnykh zavedenii Pishchevaia tekhnologiia: 154-155

Townsend, T.R.; Moses, H., 1993: Continuous quality improvement: much-needed scrutiny. Clinical Performance and Quality Health Care 1(2): 67-68

Nakamura S.; Itoh S.; Kuroiwa T., 1986: Behavior of chloroplast nucleus during chloroplast development and degeneration in chlamydomonas reinhardii. Changes in morphology of chloroplast nuclei (cp-nuclei), total cp-DNA content, number of cp-nuclei, oxygen evolution activity and chlorophyll (a and b) content were examined during the degeneration and development of chloroplasts, using Chlamydomo...

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Kubota, M., 1985: Autoradiographic study of iron transport in rat incisor enamel. An autoradiographic study using 55Fe was performed to investigate the distribution of iron in the ameloblasts and enamel of rat incisors. The study of methodological problems of 55Fe autoradiography revealed that in the undecalcified sections, the...

Keeman, H.; Harris, G.C., 2016: An audit of frontline (F)ISH testing outside the Australian setting on HER2 positivity rates in breast cancer and the impact of the 2013 ASCO guidelines. Pathology 48 Suppl 1: S61-S61