geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of proteins that bind tightly to pre-mRNA during in vitro splicing


, : Identification of proteins that bind tightly to pre-mRNA during in vitro splicing. Proceedings of the National Academy of Sciences of the United States of America 83(11): 3718-3722

Incubation of a human beta-globin pre-mRNA in a HeLa cell nuclear extract under conditions permissive for efficient splicing resulted in the assembly of the RNA into ribonucleoprotein (RNP) complexes. This RNP formation occurred largely within the characteristic lag period that precedes splicing. Two classes of RNP were detected by the criterion of their stability in Cs2SO4 gradients. One was unstable and contained mainly aberrant RNA cleavage products. The other class of RNP complexes comprised 50-85% of the beta-globin RNA, formed only under splicing-permissive conditions, was stable in Cs2SO4 gradients, and contained both unspliced pre-mRNA molecules and the lariat intron 1-exon 2 splicing intermediate. This latter class of RNP complexes banded at approximately equal to 1.30 g/cm3, a density very similar to that of native heterogeneous nuclear RNP particles that contain pre-mRNA. RNA-protein crosslinking revealed major proteins of Mr approximately equal to 38,000 and 41,000 in the stable class of RNP. The use of antibodies specific for heterogeneous nuclear RNP core proteins and for small nuclear RNA-associated proteins, in conjunction with [32P]RNA-protein crosslinking, revealed polypeptides having the molecular weights of both sets of antigens. These results show that both heterogeneous nuclear RNP particle core proteins and small nuclear RNA-associated proteins bind tightly to pre-mRNA during splicing in vitro.

US$29.90

PMID: 3459150


Other references

Balraj Singh; Vijai Singh; Singh, R.P.; Srivastava, B.K., 1998: Economic prospects of vegetable intercropping in young eucalypts plantation. Seedlings of Eucalyptus sp. were transplanted in September 1987 at 3.0x1.5 m spacing [in Uttar Pradesh, India]. In 1987-88 and 1988-89, 4 crop rotations were grown either in the Eucalyptus plantation or on open ground. Productivity and economics o...

Hagino, H.; Takada, T.; Kunimi, H.; Sakamoto, K., 2007: Characterization and source presumption of wintertime submicron organic aerosols at Saitama, Japan, using the Aerodyne aerosol mass spectrometer. The chemical composition and size distribution of submicron aerosols were analyzed at a suburban site at Saitama, Japan, in the winter of 2004/2005, using an Aerodyne aerosol mass spectrometer. Although organics and nitrate were the dominant speci...

Fadel, P., 2003: Respect for bodily integrity: a Catholic perspective on circumcision in Catholic hospitals. American Journal of Bioethics 3(2): W9-W9

Pinnola, I.M., 1988: Chestnut production in relation to climate. Climatic data for Cuneo (N. Italy), Arezzo (central Italy) and Cosenza (S. Italy) were studied for the years 1960-72 to examine the relationship between climate and nut production. Five-day mean values for maximum and minimum air temperatures and...

Sasaki, Kazuo, 1993: Study on integrative function of the frontal association cortex of monkeys and humans. Neuroscience Research Supplement 0(18): S1

Wang, Q.; Raja, K.S.; Lin, T.; Johnson, J.E.; Finn, M.G., 2002: Cowpea Mosaic Virus as a natural nanochemical building block. Abstracts of Papers American Chemical Society 224(1-2): COLL 2

Kalra, N.; Chaudhary, S.; Sanghi, S., 2005: Non-syndrome multiple supplemental supernumerary teeth. Multiple supernumerary teeth without any associated systemic conditions or syndromes are not common. We document a case of non-syndrome multiple supplemental supernumerary teeth in mandibular premolar region of an adolescent male patient, an incid...

Siegel, R.A.; Weidenfeld, J.; Feldman, S.; Conforti, N.; Chowers, I., 1981: Neural pathways mediating basal and stress-induced secretion of luteinizing hormone, follicle-stimulating hormone, and testosterone in the rat. Adult male rats, intact or bearing complete, anterior, or posterior hypothalamic deafferentations (CHD, AHD, or PHD, respectively) or bilateral medial forebrain bundle (MFB) lesions, were acutely exposed to visual, audiogenic, or thermal stress. T...

Ljungstrom, B., 1964: Treatment of spontaneous hypoglycaemia with glucagon. A diet high in protein and low in carbohydrate was inadequate for treating severe spontaneous functional hypoglycaemia in a 71-year-old woman. Injections of 0.5 mg glucagon 3 to 4 times daily eliminated the attacks. It is suggested that "func...

Anonymous, 2003: Clear, practical and concise Fiona Horrox Clear, practical and concise Whurr Publishers 440pp £35 1 86156 244 6 1861562446 [Formula: see text. NlmCategory="UNASSIGNED">This comprehensive text has been written by a nurse for nurses. The complex subject of heart disease in neonates and children has been approached in a clear, practical and concise way throughout. The first cha...