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Immunoenzyme test system based on antibody F(ab)2-fragments for detecting the NP protein of the influenza virus


, : Immunoenzyme test system based on antibody F(ab)2-fragments for detecting the NP protein of the influenza virus. Voprosy Virusologii 33(1): 18-21

A test system has been proposed for the detection of influenza A virus NP protein by solid-phase enzyme immunoassay in a modified system of "double" antibodies: the primary antibodies were F(ab)2-fragments of immunoglobulin class G isolated from rabbit antiserum specific for NP protein which was also used as the source of secondary antibodies. The antigen-bound antibodies were detected by protein A (from St. aureus) conjugate with horseradish peroxidase. The high specificity and sensitivity of the test system (0.3 ng/ml NP protein) and a minimal level of nonspecific reactions were demonstrated. The advantages of this method are discussed.

US$29.90

PMID: 3285591


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