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Immunoenzyme test system based on antibody F(ab)2-fragments for detecting the NP protein of the influenza virus

, : Immunoenzyme test system based on antibody F(ab)2-fragments for detecting the NP protein of the influenza virus. Voprosy Virusologii 33(1): 18-21

A test system has been proposed for the detection of influenza A virus NP protein by solid-phase enzyme immunoassay in a modified system of "double" antibodies: the primary antibodies were F(ab)2-fragments of immunoglobulin class G isolated from rabbit antiserum specific for NP protein which was also used as the source of secondary antibodies. The antigen-bound antibodies were detected by protein A (from St. aureus) conjugate with horseradish peroxidase. The high specificity and sensitivity of the test system (0.3 ng/ml NP protein) and a minimal level of nonspecific reactions were demonstrated. The advantages of this method are discussed.


PMID: 3285591

Other references

Skurikhin, V.N.; Toikka, V.S., 1986: Effect of feeding with fish solubles conserved with formalin on metabolism and productivity in lactating cows. A control group of cows received a diet containing digestible protein at 113 g/feed unit, fibre 18.2 and fat 2.47% DM, and had a calcium:phosphorus ratio of 1.6:1; 1 feed unit contained 1.27 kg DM. Cows in the test group were fed as those in the c...

Tucker, M., 1999: Can solar cooking save the forests?. Cooking with the sun has become a potentially viable substitute for fuelwood in food preparation in much of the developing world. Fuelwood scarcity is a growing problem that has thus far been poorly addressed. Solar cooking is one possible solutio...

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Strenger, A., 1950: Eine funktionsanatomische Untersuchung einiger Dermapterencerci. Zoologische Jahrbuecher Jena Anatomie, 70: 576-600

Wimalaratne, S.M.; Halstead, M.D.B.; Lloyd, C.M.; Cooling, M.T.; Crampin, E.J.; Nielsen, P.F., 2009: Facilitating modularity and reuse: guidelines for structuring CellML 1.1 models by isolating common biophysical concepts. The CellML language was developed in response to the need for a high-level language to represent and exchange mathematical models of biological processes. The flexible structure of CellML allows modellers to construct mathematical models of the sa...

Dimonte, V., 1993: A window on the past: lay nurses and nun nurses in hospital modernization at the start of the 20th century. The presence of nuns in Italian hospitals raised hot debates since the beginning of the century. Though recognised by a large majority as ideal nurses, for the devotion and the amount of time spent working in the hospital, physicians, feminist mov...

Smith, G.P.; Gibbs, J.; Jerome, C.; Pi-Sunyer, F.X.; Kissileff, H.R.; Thornton, J., 1981: The satiety effect of cholecystokinin: a progress report. The satiety effect of cholecystokinin (CCK) that was first observed in rats has now been extended to chickens, rabbits, pigs, sheep, rhesus monkeys, lean mice, genetically obese mice and rats, neurologically obese rats, lean men and women, and obe...

Moumile, K.; Merckx, J.; Glorion, C.; Pouliquen, J.C.; Berche, P.; Ferroni, A., 2005: Bacterial aetiology of acute osteoarticular infections in children. Aim: To study the bacterial aetiology of acute osteoarticular infections in children and to analyse the efficiency of culture methods. Methods: Bacteriological data of 407 cases of clinically suspected osteoarticular infections affecting 406 child...

Ghods, A.J.; Ossareh, S., 2002: Detection and treatment of coronary artery disease in renal transplantation candidates. Transplantation Proceedings 34(6): 2415-2417

Alvarez D.S.ntos M.; Florenzano A.H.; Hurtoado M.B.; Butcher Lopez E.A.; Gonzalez Quiroga G.; Santos Flores J.R., 1985: Xx male syndrome. Boletin Medico del Hospital Infantil de Mexico 42(6): 393-396