geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

In vitro methane and methyl coenzyme M formation from acetate: evidence that acetyl-CoA is the required intermediate activated form of acetate


, : In vitro methane and methyl coenzyme M formation from acetate: evidence that acetyl-CoA is the required intermediate activated form of acetate. Biochemical and Biophysical Research Communications 147(1): 254-258

Buffer-soluble extracts of acetate-grown Methanosarcina barkeri catalyzed methanogenesis from acetate in the presence of hydrogen and ATP. The rates of methane formation from either acetate plus ATP, or acetylphosphate without ATP added, were approximately doubled by the addition of coenzyme A (CoA). In vitro methyl group transfer from [2-14C]acetate to form [14CH3]methyl coenzyme M (14CH3S-CoM) was monitored by causing the accumulation of 14CH3S-CoM (14CH3-SCH2CH2SO3-) in the presence of 2-bromoethanesulfonate. The rate of 14CH3S-CoM formation was increased 2.5-fold by 0.2 mM CoA.

US$29.90

PMID: 3115259


Other references

John P., 1987: Paracoccus as a free living mitochondrion. Lee, J J And J F Fredrick (Ed ) Annals Of The New York Academy Of Sciences, Vol 503 Endocytobiology, Iii; International Colloquium, New York, New York, Usa, June 10-12, 1986 Xii+590p The New York Academy Of Sciences: New York, New York, Usa Illus 140-150

Chen ShuChun; H.Y.nHeng, 1995: Two new species of the genus Baculum found at Mt. Tianmu, Zhejiang, China. Descriptions are given of Baculum tianmushanense sp. nov. and Baculum brachycerum sp. nov.

McLeod John D., 1993: The search for oil and gas in Idaho. AAPG Bulletin: , Pages 1455. 1993.

Sarkis-Onofre, R.; Poletto-Neto, Vório.; Cenci, M.Sérgio.; Pereira-Cenci, T.; Moher, D., 2017: Impact of the CONSORT Statement endorsement in the completeness of reporting of randomized clinical trials in restorative dentistry. The aim of this study was to assess if journal endorsement of the CONSORT Statement is associated with improved completeness of reporting of randomized controlled trials (RCTs) in restorative dentistry. RCTs in restorative dentistry published in t...

Knychalska Karwan Z.; Pawlicki R.; Jakob Dolezal K.; Karwan T., 1982: Hard teeth tissues in rats with experimental rickets examinations with scanning electron microscope and micro analyzer. Experiments were carried out on Wistar rats with experimentally induced rickets. Radiological examinations of the jaws, tooth analysis in the microanalyzer for the distribution of fundamental elements and ultrastructure observations in the scannin...

Palmer, F.J.; D.C.rle, D.J., 1976: Arterial vasoconstrictor therapy for bleeding oesophageal varices. Upper gastrointestinal bleeding in patients with hepatic cirrhosis carries considerable mortality. Difficulties are encountered both in determining the source of bleeding and in controlling blood loss. The techniques of selective visceral angiogra...

Ward, C.; Glass, N.; Ford, R., 2014: Care in the home for seriously ill children with complex needs: A narrative literature review. This article reviews contemporary literature on home care of the seriously ill child with complex care needs and the sick child/parent dyad. The literature search revealed three major themes, namely the increasing acuity of child illness, the evol...

Eisenberg, G.M.; Flippin, H.F.; Kayser, H.L.; Nadel, J.; Sathavara, S.; Spivack, A.; Weiss, W., 1956: Klebsiella in respiratory disease. Klebsiella pneumoniae and Aerobacter aeorgenes cannot be distinguished on any basis. Since 1952, organisms fitting the criteria of Kauffmann have been differentiated by capsular type. Serotypes 1 to 10 were distinguished. It is concluded that Kleb...

Gill, T., 1899: Larval stage of the Eel. Science, 2(ix): 820

Garcia-Sancho, J.; Giraldez, F.; Belmonte, C., 1978: Absence of apparent cell pH variations during hypoxia in the carotid body chemoreceptors in vitro. Neuroscience Letters 10(3): 247-249