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Inhibition of neutrophil chemotaxis by elastase-generated IgG fragments


, : Inhibition of neutrophil chemotaxis by elastase-generated IgG fragments. Scandinavian Journal of Immunology 34(3): 359-364

IgG1 is cleaved in vitro by granulocyte elastase into Fc and Fab fragments. The elastase-specific Fc fragment has been previously detected in vivo. Biological activity of the fragments has been described in modulating neutrophil oxidative metabolism and enzyme release. To investigate further effects granulocyte chemotaxis (CT) was tested. The CT was assayed in Boyden chambers and the chemotactic index (CI) was calculated which represents the mean distance travelled by the activated cells. Stimulation of leucocyte CT by casein, activated serum and FMLP gives maximal values of delta CI = 46.7, 26.4 and 7.2 microns, respectively. Native IgG1 and the elastase-produced IgG fragments do not stimulate leucocyte CT. FMLP-stimulated CT is specifically inhibited by the elastase-produced Fc fragments. Addition of 7 nmol Fc to stimulus concentrations of 16 to 125 nM FMLP results in total inhibition of chemotaxis demonstrated by CI values which are lower than those for unstimulated cells. The inhibition of CT is concentration dependent in the range of 2 to 7 nmol Fc/10(6) PMN. Number and affinity of FMLP receptors are not influenced by Fc fragments, so Fc binds neither to FMLP nor the FMLP receptor. CT stimulated by casein shows a large portion of chemokinesis. Only at suboptimal casein concentrations do Fc and IgG have an inhibitory effect on CT (0.63 mg casein/ml, 10 nmol peptide/10(6) PMN). C5a-stimulated CT is not influenced by IgG or IgG fragments which indicates that the samples are not cytotoxic. So the FMLP and casein-stimulated CT is specifically inhibited by the elastase-produced Fc fragments in a low concentration range.

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PMID: 1882190


Other references

Lionnet, G.R.E., 1989: Washing with syrup in A batch centrifugals. The possibility of washing massecuites with syrup during centrifuging was tested. In the laboratory, artificial massecuites were washed with either 40 degrees Bx syrup or water. At one factory, A massecuites were washed with 50 degrees Bx syrup; a...

Bookchin, R.M.; Ortiz, O.E.; Freeman, C.J.; Lew, V.L., 1989: Predictions and tests of a new integrated reticulocyte model: implications for dehydration of sickle cells. Progress in Clinical and Biological Research 319: 615-627

Andreoli, J.M.; Jang, S.I.g; Tarcsa, E.; Coticchia, C.M.; Steinert, P.M.; Markova, N., 1997: The regulation of expression of the human trichohyalin gene in cultured hair follicles. Journal of Investigative Dermatology 108(4): 630

Vanetti, A.; Levy, J.P.; Fourati, M.; Ben-Ismail, M.; Daumet, P., 1978: Total anomalous pulmonary venous return: apropos of an unusual case. A case is reported in which the entire pulmonary venous return was abnormal, being retro-cardiac. Unusual features were the age at which the abnormality was discovered (14 years), and the anatomical type of abnormality of pulmonary venous return....

Wingfield, A.; Byrnes, D.L., 1972: Decay of information in short-term memory. Self-paced recall in Broadbent's simultaneous listening task shows particular temporal patterns associated with report strategies. Accuracy is a decreasing function of the interval between presentation and report of an item, irrespective of r...

Stockwell, R.A., 1974: Fine structure and macromolecular organization of connective tissue. Transactions of the Ophthalmological Societies of the United Kingdom 94(3): 648-662

Almeida, Jão.Paulo.; Ruiz-Treviño, A.S.; Liang, B.; Omay, S.B.; Shetty, S.R.; Chen, Y-Ning.; Anand, V.K.; Grover, K.; Christos, P.; Schwartz, T.H., 2017: Reoperation for growth hormone-secreting pituitary adenomas: report on an endonasal endoscopic series with a systematic review and meta-analysis of the literature. OBJECTIVE Surgery is generally the first-line therapy for acromegaly. For patients with residual or recurrent tumors, several treatment options exist, including repeat surgery, medical therapy, and radiation. Reoperation for recurrent acromegaly h...

Vasil'-Ev, Ai, 1978: Quality of corn green matter as a feeding area and fertilization background under Siberian conditions. Soviet agriculture sciences: 2) 8-9

Muzio, L.G.; Ohashi, J.P., 1979: The RN student--unique characteristics, unique needs. Nursing Outlook 27(8): 528-532

Tukendorf, A.S.bczynski, W.; Baszynski, T., 1981: Participation of beta-carotene in reactivation of PSI of heptane-extracted spinach chloroplasts. Photosynthesis research(3): 153-166