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Interaction of rabbit muscle enolase and 3-phosphoglycerate mutase studied by ELISA and by batch gel filtration

, : Interaction of rabbit muscle enolase and 3-phosphoglycerate mutase studied by ELISA and by batch gel filtration. Archives of Biochemistry and Biophysics 296(2): 650-653

The interaction of rabbit skeletal muscle enolase and 3-phosphoglycerate mutase was detected by an ELISA test, a batch gel-filtration technique, and fluorescence anisotropy measurements, and the activity of enolase was determined to be a function of mutase concentration. The apparent dissociation constant of this enzyme complex is approximately 1 microM. This value seems to be independent of the presence (in fluorescence anisotropy measurements) or the absence (in activity as well as in ELISA experiments) of fluorescein isothiocyanate used widely as a label for determining the complex formation between enzymes in fluorescence anisotropy measurements.


PMID: 1321593

Other references

Shun ko V.V., 1987: The genesis of apatite mineralization in the Kazankov District, central Ukrainian Shield. Dopovidi Akademiyi Nauk Ukrayins'koyi RSR, Seriya B: Geologichni, Khimichni ta Biologichni Nauki 1987(4): 34-37

Hunter, S., 1957: Stewart Hunter. New Zealand Medical Journal 56(313): 271-271

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Petri, Karl., 1912: Siebenburgens Kafer-fauna auf Grund ihrer Erforschung bis zum Jahre 1911. (Hrsg. v. Siebenburg. Verein f. Naturwiss.) Hermannstadte (Druck v. J. Drotleff) Komm-Verl. Unknown

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D.C.rvalho, M.; Nogueira, A.; Pinto, A.; Miguens, J.; Sales Luis, M.L., 1999: Reflex sympathetic dystrophy associated with amyotrophic lateral sclerosis. Reflex sympathetic dystrophy (RSD) is a syndrome characterised by severe distal pain and vasomotor changes. It is believed to be caused by sympathetic nervous system overactivity. Trauma is the most frequent precipitant event. An association with...

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Warr, W.A., 2012: Scientific workflow systems: Pipeline Pilot and KNIME. Journal of Computer-Aided Molecular Design 26(7): 801-804

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