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Ionophore A23187 induces a refractory state in thrombin-activated release of inositol phosphates

, : Ionophore A23187 induces a refractory state in thrombin-activated release of inositol phosphates. Biochemical Journal 238(3): 709-714

The phosphatidylinositol cycle has been proposed to be involved in the regulation of platelet functionality through the control of cytoplasmic Ca2+ levels. However, the requirements of phospholipase C for Ca2+ has not yet been elucidated in intact platelets. The primary purpose of the present study was to investigate the Ca2+ requirements of this enzyme in platelets from miniature swine by taking advantage of the permeabilizing properties of the ionophore A23187. Our results strongly suggest that the treatment of platelets with A23187 induces a refractory state in thrombin-stimulated release of inositol phosphates while 5-hydroxytryptamine (serotonin)-secretory capacity in response to thrombin remained constant. This refractory state seems to be dependent on some cytochalasin-inhibitable cytoskeletal phenomena.


PMID: 3099773

Other references

Verona, O., 1960: The special ecological environment in the boron-manufacturing centre at Larderello, and the boron tolerance of some plant species.. Data are reported on the B supplied by precipitation, deposited on and taken up by leaves, and contained in soil near B-producing factories.

Hartog, A.; Houmes, R.J.; Verbrugge, S.J.; Erdmann, W.; Lachmann, B., 1997: Partial liquid ventilation and inhaled nitric oxide have a cumulative effect in improving arterial oxygenation in experimental ARDS. Advances in Experimental Medicine and Biology 428: 281-283

Sussman, V., 1985: Cantaloupes without calamity. Organic gardening 32(5): 56-59

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