geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Lens-specific expression of the chloramphenicol acetyltransferase gene promoted by 5' flanking sequences of the murine alpha A-crystallin gene in explanted chicken lens epithelia


, : Lens-specific expression of the chloramphenicol acetyltransferase gene promoted by 5' flanking sequences of the murine alpha A-crystallin gene in explanted chicken lens epithelia. Proceedings of the National Academy of Sciences of the United States of America 82(8): 2334-2338

We have developed a system using explanted embryonic chicken lens epithelia to express foreign recombinant genes containing crystallin DNA regulatory sequences introduced by calcium phosphate transfection. Optimal results were obtained with lens epithelia from 14-day embryos transfected 1 day after explantation and assayed 3 days later. When DNA sequences (-364 to +45) of the murine alpha A-crystallin gene were inserted in the pSVO-CAT expression vector of Gorman et al. [Gorman, C. M., Moffat, L. F. & Howard, B. H. (1982) Mol. Cell. Biol. 2, 1044-1051] in the same orientation as in the crystallin gene, they promoted chloramphenicol acetyltransferase (CAT; EC 2.3.1.28) activity in the transfected epithelia. Sequences 87 to 364 base pairs upstream from the murine gene cap site were required for CAT gene expression. These crystallin gene regulatory sequences did not promote CAT expression in primary cultures of embryonic chicken fibroblasts or other nonlens cells. By contrast, the long terminal repeat of Rous sarcoma virus and the early promoter of simian virus 40 promoted CAT activity in lens and nonlens cells. Our experiments thus demonstrate that the explanted embryonic chicken lens epithelium is an advantageous recipient for identifying lens-cell-specific regulatory sequences of crystallin genes and implicate a DNA region upstream of the "TATA box" for regulation of the murine alpha A-crystallin gene. These experiments also suggest that explanted epithelia from other tissues may be useful for studying the expression of foreign genes.

US$29.90

PMID: 3857584


Other references

Olmi, M., 1976: Dryops kaszabi new species from hungary coleoptera dryopidae. Dryops kaszabi from Hungary is described [and compared to D. griseus Erichson]. Types are deposited in the Hungarian Natural History Museum, Budapest.

Hébert, R., 2008: What's new in nicotine & tobacco research?. Nicotine & Tobacco Research 10(4): 559-566

Ionescu, T.; Cazacu, E.; Georgian, P.; Dăscălescu, C., 1969: Some methodological aspects of the nosologic liquidation of diphtheria. Revista Medico-Chirurgicala A Societatii de Medici Si Naturalisti Din Iasi 73(4): 949-954

Fife Donald L.; Bryant Mark E., 1982: Significance of Neogene phosphorites in Capistrano Embayment, Southern California. AAPG Bulletin 66(10): 1688

Vivirito, G., 1977: Should the dentist be allowed to advertise?. Dental Student 55(4): 45-47

Izadpanah, R.; Kaushal, D.; Kriedt, C.; Tsien, F.; Patel, B.; Dufour, J.; Bunnell, B.A., 2008: Long-term in vitro expansion alters the biology of adult mesenchymal stem cells. Mesenchymal stem cells (MSC) derived from bone marrow stem cells (BMSC) and adipose tissue stem cells (ASC) of humans and rhesus macaques were evaluated for their cell cycle properties during protracted culture in vitro. Human ASCs (hASC) and rhe...

Lai, J.; Sun, H-xia.; Jie, Y-Sheng.; Zhang, K.; Ke, W-min., 2014: Serum HBsAg level and its clinical significance in lamivudine treatment for patients with HBeAg-negative acute-on-chronic liver failure. To investigate the dynamics and clinical significance of hepatitis B surface antigen (HBsAg) levels in hepatitis B e antigen (HBeAg)-negative acute-on-chronic liver failure (ACLF) patients receiving lamivudine. Fifty-nine nucleoside-naïve patient...

Garcia-Closas, M.; Hankinson, S., E.; Ho, S.-Mei; Malins, D., C.; Polissar, N., L.; Schaefer, S., N.; Su, Y.; Vinson, M., A., 2000: Chapter 9: Factors critical to the design and execution of epidemiologic studies and description of an innovative technology to follow the progression from normal to cancer tissue. The results obtained from experimental studies of estrogen carcinogenesis need validation in epidemiologic studies. Such studies present additional challenges, however, because variations in human populations are much greater than those in experim...

Pittinger, C., 1970: Ionic calcium. Crc Critical Reviews in Clinical Laboratory Sciences 1(2): 351-379

Iván, Gábor.; Szabadka, Zán.; Grolmusz, V., 2008: Being a binding site: characterizing residue composition of binding sites on proteins. The Protein Data Bank contains the description of more than 45,000 three-dimensional protein and nucleic-acid structures today. Started to exist as the computer-readable depository of crystallographic data complementing printed articles, the prope...