geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Mapping of the multiple regulatory sites for putP and putA expression in the putC region of Escherichia coli


, : Mapping of the multiple regulatory sites for putP and putA expression in the putC region of Escherichia coli. Molecular & General Genetics 214(3): 379-388

The effects of regulatory proteins on the expression of putP and putA were studied using put-lacZ fusion genes. The expression of the putP-lacZ gene was activated by the glnG gene product and the catabolite gene activator protein (CAP). The putA gene product inhibited activation of putP-lacZ gene expression by CAP or the glnG gene product and its inhibition was greater in the absence of proline. The expression of the putA-lacZ gene was activated by CAP and repressed by the glnG gene product. The putA gene product acted as a repressor in the absence of proline, but not in its presence. Studies using put-lacZ fusion genes with upstream deletions showed that the region required for the activation of putP by CAP was within 234 bp upstream of the translational initiation site and that that for the activation of putA was within 107 bp upstream of the translational initiation site of the putA gene. This supported the suggested locations of CAP binding sites. The region required for induction of putP and putA expression by proline was located at the HpaI site 182 bp upstream of the translational starting site of putA, suggesting that a sequence of dyad symmetry located 1 bp to the left of the HpaI site is a candidate for the binding site of the putA gene product.

US$29.90

PMID: 2464125


Other references

Leon, L.A., 1956: Contribucion del Dr. Paul Rivet al conocimiento cientifico de la Republica del Ecuador. Boletin de Informaciones Cientificas Nacionales Quito, 876: 681-706

Emery, C.A.; Kang, J.; Schneider, K.J.; Meeuwisse, W.H., 2012: Risk of injury and concussion associated with team performance and penalty minutes in competitive youth ice hockey. To determine if there is an association between the risk of all injury or concussion and win–loss records or penalty minutes in competitive youth ice hockey players (ages 11–14). , setting, participantsThis is a secondary data analysis...

Levi, D.; Saarinen, J., 2004: Perception of mirror symmetry in amblyopic vision. Mirror symmetry is ubiquitous in natural visual scenes, and detection of mirror symmetry seems to be a global, automatic, effortless and important aspect of visual perception. The perception of mirror symmetry has not been studied in humans with a...

Roemer Ferdinand, 1850: Ueber Stephanocrinus, eine fossile Crinoiden-Gattung aus der Familie der Cystideen. Arch Naturg 16(1): 365-375

Perreau, P.; Joubaud, F.; Simard, C.; Gazeau, O., 1972: Gougerot-Sjögren syndrome and glomerulonephritis. La Semaine des Hopitaux 48(14): 973-978

Rokach J.; Tagari P.; Ethier D.; Foster A.; Delorme D.; Malo J.L.; Cartier A.; Manning P.; O'byrne P.M.; Girard Y., 1989: Identification and quantitation of leukotriene metabolites in animal and man. Zor, U , Z Noar And A Danon (Ed ) New Trends in Lipid Mediators Research, Vol 3 Leukotrienes And Prostanoids in Health And Disease; 2nd International Conference, Jerusalem, Israel, October 9-14, 1988 X+345p S Karger Ag: Basel, Switzerland; New York, New York, Usa Illus 187-193

Vaarama, A., 1949: Spindle abnormalities and variation in chromosome number in Ribes nigrum. A num-ber of colchieine-induced tetraploid Ribes nigrum bushes and their c2-progeny raised from seed were studied. The chromo-some number in the somatic cells has varied from 4 to 32. The formation of 2 separate and independent spindles in one cel...

Faleev N.G.; Ruvinov S.B.; Zakomyrdina L.N.; Sakharova I.S.; Torchinskii Y.M.; Belikov V.M., 1991: Factors determining the binding efficiency of tryptophanase and amino acids. Inhibition of tryptophanase-catalyzed decomposition of S-(o-nitrophenyl)-L-cysteine by a variety of amino acids has been investigated. For amino acids similar to the natural substrate and for those having minimal steric requirements for the side c...

de Simone, G.; Roman, M.J.; Daniels, S.R.; Mureddu, G.; Kimball, T.R.; Greco, R.; Devereux, R.B., 1997: Age-related changes in total arterial capacitance from birth to maturity in a normotensive population. We evaluated the effect of body growth and aging on the ratio of echocardiographic (Teichholz) stroke volume to pulse pressure (SV/PP ratio) in 373 normal-weight, normotensive children to adolescents (1 day to 17 years old; 166 girls, 87 nonwhite)...

Romero Sueldo, M.; Cuezzo, F., 2001: Presence of Paratrechina silvestrii (Hymenoptera: Formicidae), possible natural enemy of Doru lineare (Dermaptera: Forficulidae) in maize crops. Doru lineare is a predator of the maize pest Spodoptera frugiperda in Argentina. A study was carried out between October 1999 and January 2000 in Tucaman province. Paratrechina silvestrii was identified for the first time feeding on ova and nymphs...