geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Molecular weight of mitochondrial type B MAO in various organs of guinea pig


, : Molecular weight of mitochondrial type B MAO in various organs of guinea pig. Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica 85(1): 23-31

Molecular weights of mitochondrial type B monoamine oxidase (MAO) in guinea pig brain, liver and kidney were estimated, and their identities and multiplicity were studied. We ascertained what concentration of 3H-pargyline bound to type B MAO specifically from the inhibition curve toward serotonin (5-HT) and beta-phenylethylamine (beta-PEA) by pargyline. Pargyline irreversibly binds to FAD in MAO at a one to one molecular ratio. 3H-pargyline bound to type B MAO specifically and irreversibly by incubation for 5 hr at 37 degrees C, and SDS-disc electrophoresis was carried out using 3H-pargyline as a tracer. The molecular weight of MAO was estimated after specific binding of pargyline was corrected for non-specific binding. The molecular weight of type B MAO in every organ was found to be 60,000, giving a single peak after solubilization with 6% SDS, but several peaks at higher molecular weight were found in each organ after solubilization with 2% SDS. In the brain, there appeared to be a peak of 100,000, and it was suggested that the MAO existed as a dimer which was composed of a FAD containing subunit and a low molecular weight subunit containing no FAD. In the liver, there appeared to be peaks of 120,000 and 240,000, and it was suggested that the MAO existed as a dimer and tetramer. In the kidney, there appeared to be a peak of 180,000, and MAO was suggested to exist as a trimer.

US$29.90

PMID: 3988165


Other references

Chiou, W.-Bin; Cheng, Y.-Yao, 2013: In broad daylight, we trust in God Brightness, the salience of morality, and ethical behavior. Based on metaphorical associations between light and goodness, we hypothesized that experiencing brightness increases the salience of moral considerations and the likelihood of engaging in ethical behavior. The results of three experiments support...

Tricot, M.; Houssier, C.; Desreux, V., 1975: Electro-optical studies on synthetic polyelectrolytes. I. Ultraviolet electric dichroism of poly-N-alkylvinylpyridinium bromides in aqueous solution. The electric dichroism of aqueous solutions of poly-2- and 4-vinylpyridinium bromides has been measured in the 220 to 300 nm region. From the knowledge of the assignments of the absorption bands, the orientation of the pyridinium ring with respect...

Simon, E., 1888: Descriptions d'especes et de genres nouveaux de Madagascar et de Mayotte. Annales de la Societe Entomologique de France, VIII 223-236

Hansing, C.E.; Marquardt, J.P.; Sutton, D.M.; York, J.D., 2016: Balloon-Occluded Retrograde Transvenous Obliteration of a Gastric Vascular Malformation: An Innovative Approach to Treatment of a Rare Condition. NlmCategory="UNASSIGNED">Arteriovenous malformations (AVMs) are a high-flow form of a vascular malformation, which can be found anywhere in the body. While historically treated surgically, a multidisciplinary approach utilizing multip...

Shahgholi, E.; Mollaian, M.; Haghshenas, Z.; Honarmand, M., 2012: Congenital rhabdomyosarcoma, central precocious puberty, hemihypertrophy and hypophosphatemic rickets associated with epidermal nevus syndrome. We describe a newborn girl with right-sided extended epidermal nevus, congenital rhabdomyosarcoma of the inguinal area at birth who had developed central precocious puberty, hemihypertrophy and vitamin D3-responsive hypophosphatemic rickets at the...

Waymouth, Charity, 1956: A rapid quantitative hematocrit method for measuring increase in cell population of strain L cells culti-vated in serum-free nutrient solutions. Data are presented to show that the growth of replicate tissue cultures of strain L cells in serum-free nutrient solutions can be evaluated simply and rapidly by serial measurements of the total volume of cells from 1 Carrel flask, using a Van All...

Tanaka, A.; Okuzumi, H.; Hosokawa, T.; Murai, N., 1998: Sex differences in facial skin temperature when exposed to darkness with and without warning. The changes in skin temperature under anxiety were investigated by noninvasive measurements. The nasal skin temperature of 20 subjects was assessed by thermography. The measurement of the skin temperature by thermistor causes stress in subjects. T...

Frerker, C.; Schäfer, U.; Kuck, K.H., 2010: Mitral valve regurgitation in advanced heart failure: interventional or surgical treatment? For interventional treatment. Deutsche Medizinische Wochenschrift 135(13): 624-624

Evdokimov, A., 1973: Grafting and regrafting of fruit trees. Opty Soderzhaniia Sada: 8-51

Gvozdenovic, M.P., 1961: Entamoeba histolytica and other intestinal parasites in a mountain village. Medicinski Arhiv 15: 13-20