+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) does not modify the angiotensin II-stimulated calcium signal in cultured bovine glomerulosa cells

, : N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) does not modify the angiotensin II-stimulated calcium signal in cultured bovine glomerulosa cells. Life Sciences 48(1): 27-35

Studies were performed to determine if the sustained elevation in [Ca2+]c noted previously in glomerulosa cells in response to Ang II resulted from the presence of HEPES in the experimental medium. At confluence, primary cultures of bovine glomerulosa cells were maintained for 24-30 h in the presence of either 14 mM NaHCO3/5% CO2 or 25 mM HEPES/4 mM NaHCO3/air. During subsequent experimental periods, cells were incubated in the presence of the corresponding or reciprocal buffer, and the effects of Ang II on [Ca2+]c were monitored by fura 2 fluorescence. Increases in [Ca2+]c produced by Ang II in cells continuously maintained in either HCO3(-) - or HEPES-buffered media were similar, and with the same monolayer the nature of the Ang II-stimulated Ca2+ signal was independent of the buffer employed. Moreover, the Ang II-stimulated Ca2+ signal was not significantly affected by the removal of HCO3- from the superfusate. These results indicate that the sustained increase in [Ca2+]c is not an artifact introduced by the use of HEPES as an experimental buffer, but rather a normal component of the Ang II-stimulated Ca2+ signal.


PMID: 1986182

Other references

Chunwarin, W., 1976: Physical properties of three Thai bamboos. Bambusa arundinacea, Thyrsostachys siamensis and Dendrocalamus membranaceus.

Hales, T.C.; Roering, J.J., 2009: A frost buzzsaw mechanism for erosion of the eastern Southern Alps, New Zealand. In the Southern Alps, New Zealand, large gradients in precipitation (<1 to 12 m year(-1) ) and rock uplift (<1 to 10 mm year(-1) ) produce distinct post-glacial geomorphic domains in which landslide-driven sediment production dominates in th...

Tisseau, M.A., 1959: Deficiency of copper and zinc in pineapples: crook-neck disease. Symptoms are described, and curative measures are given in detail. These involve spraying the leaves with 1% zinc sulfate, using 2, 000 1. of solution per ha. and repeating the treatment if necessary, and spraying the soil round the plants with 1....

Danilczyk U.; Olley P.M.; Jackowski G., 1987: Evidence for noncoordinated expression of cardiac myosin heavy and light chains. Journal of Muscle Research & Cell Motility 8(1): 82

O'Dowd, A., 2009: Doctors warn against removing benefits from alcoholics who refuse treatment. Bmj 338: B1591-B1591

Donovan, D.A.; Bingham, B.L.; Farren, H.M.; Gallardo, R.; Vigilant, V.L., 2002: Effects of sponge encrustation on the swimming behaviour, energetics and morphometry of the scallop Chlamys hastata. The effect of sponge encrustation on swimming ability of Chlamys hastata was determined by investigating swimming behaviour, scallop morphometry, and energy expended during swimming with and without commensal epibionts. Scallops swam significantly...

Kim, H.; Im, S.H.; Ambalavanar, R.; Enquist, L.W.; Ludlow, C.L., 2003: Transynaptic connections of laryngeal mucosal afferents in the medulla. Stimulation of laryngeal mucosal afferents elicits protective adductor reflexes in the cat larynx. To determine the transynaptic pathway involving these afferents in the medulla, we used the Becker strain of pseudorabies virus (PRV) because of its...

Islam, M.S.; Berggren, P.O., 1998: Depolarization-evoked Ca2+ signalling in pancreatic beta-cells is amplified by calcium-induced calcium release. Diabetologia 41(SUPPL 1): A37, Aug

Romero, R.; Berry, S.; Gomez, R.; Munoz, H.; Mazor, M.; Sorokin, Y.; Ghezzi, F.; Cotton, D.B., 1995: Hematologic evidence that the human fetus participates in the process leading to preterm parturition. American Journal of Obstetrics & Gynecology 172(1 PART 2): 403

Ward David S.; Greenwald Robert M.; Srinivasan P., 1992: Strategies for groundwater model application through GIS. Pages 32 1992