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Osmotic stimulation of Na(+)-K(+)-Cl- cotransport in squid giant axon is [Cl-]i dependent


, : Osmotic stimulation of Na(+)-K(+)-Cl- cotransport in squid giant axon is [Cl-]i dependent. American Journal of Physiology 258(4 Pt 1): C749-C753

The effects of increasing extracellular osmolality on unidirectional Cl- fluxes through the Na(+)-K(+)-Cl- cotransporter were studied in internally dialyzed squid giant axons. Hyperosmotic seawater stimulated bumetanide-sensitive Cl-influx at 150 mM intracellular Cl- concentration ([Cl-]i), whereas Cl- efflux was unaffected under comparable ionic conditions. Stimulation of bumetanide-sensitive Cl- influx was proportional to the increase in extracellular osmolality. Bumetanide-sensitive Cl- influx began to increase after a latency of approximately 20 min after a stepwise increase of extracellular osmolality and continued to increase for at least 70 min. The increased bumetanide-sensitive Cl- influx measured after 65 min of exposure to hyperosmotic external fluid was a function of the intracellular Cl- concentration; stimulation by hyperosmotic external fluids was observed at physiological [Cl-]i levels (greater than 100 mM) but not at lower [Cl-]i levels. Under both normo- and hyperosmotic conditions, intracellular Cl- inhibited Na(+)-K(+)-Cl- cotransport influx in a concentration-dependent manner. However, in hyperosmotic seawater, the dose dependence of inhibition by intracellular Cl- was shifted to higher [Cl-]i values. Therefore, we conclude that hyperosmotic extracellular fluids stimulate influx via the Na(+)-K(+)-Cl- cotransport by resetting the relation between [Cl-]i and transport activity.

US$29.90

PMID: 2333955


Other references

Gervitz, L.M.; Lutherer, L.O.; Hamilton, M.E.; Fowler, J.C., 2002: Lack of central effects of peripherally administered adenosine A1 agonists on synaptic transmission in the rat hippocampus. Peripheral administration of adenosine A(1) receptor selective agonists is generally thought to protect the hippocampus against ischemic damage via central actions. We examined the effects of two peripherally administered A(1) agonists, cyclohexyl...

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Bunn, H.F., 1969: Subunit dissociation of certain abnormal human hemo globins cat dog rabbit rat guinea pig. Journal of Clinical Investigation 48(1): 126-138

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Smith, A.; Roman, E.; Howell, D.; Jones, R.; Patmore, R.; Jack, A.; Ackroyd, S.; Ali, S.; Allsup, D.; Ashcroft, J.; Bashi, R.; Bond, L.; Bynoe, G.; Bowen, D.; Carter, C.; Chapple, M.; Ciepluch, H.; Cook, G.; Cuthbert, A.; Czyz, J.; Edwards, A.; Feyler, S.; Gilleece, M.; Gilson, D.; Hall, C.; Harris, E.; Hill, A.; Hill, Q.; Hillmen, P.; Howard, M.; Jalihal, S.; Johnson, R.; Kinsey, S.; Kraemer, D.; Kwok-Williams, M.; Mcverry, T.; Moreton, P.; Munro, L.; Newton, L.; Norfolk, D.; Parapia, L.; Patil, 2010: The Haematological Malignancy Research Network (HMRN): a new information strategy for population based epidemiology and health service research. The Haematological Malignancy Research Network (HMRN) was established in 2004 to provide robust generalizable data to inform clinical practice and research. It comprises an ongoing population-based cohort of patients newly diagnosed by a single in...

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