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Ovarian cancer-associated antibodies recovered from ascites: their use for the isolation of ovarian cancer-associated antigen to produce monoclonal antibodies

, : Ovarian cancer-associated antibodies recovered from ascites: their use for the isolation of ovarian cancer-associated antigen to produce monoclonal antibodies. Gynecologic Oncology 37(1): 24-28

Immune complexes (ICs) were recovered from the ascites of a patient with stage IV endometrioid ovarian cancer by sequential precipitation with 33% saturated ammonium sulfate and 2.5% polyethylene glycol 6000 (PEG 6000), followed by affinity chromatography on protein A-Sepharose CL-4B. The IgG-containing ICs were dissociated using 8 M urea, separated by ion-exchange chromatography on Sephadex QAE-50, and subsequently analyzed for purity by immunoelectrophoresis (IEP) and radial immunodiffusion (RID). Recovered antibody was tested for reactivity by immunohistologic techniques against paraffin-embedded tumor tissue and acetone-fixed cell suspensions of epithelial tumors. The antibody which demonstrated ovarian cancer-associated activity was absorbed with antigen extracts of breast, colon, and lung cancers as well as keratin to reduce cross-reactivity. The absorbed endometrioid ovarian cancer-associated antibody (OCAAb) was used to produce an immunoadsorbent column for the recovery of tumor-associated antigens. A mouse monoclonal antibody designated FEN-1 was produced using this antigen-containing fraction, and preliminary screening has demonstrated ovarian tumor-associated reactivity. The use of autologous ICs as reagents for preparing tumor antigen-rich immunogens may provide a valuable tool in the search for tumor-associated antigens.


PMID: 2182406

Other references

Light, I.J.; Sutherland, J.M., 1968: Effect of topical antibacterial agents on the acquisition of bacteria by newborn infants. Antimicrobial Agents and ChemoTherapy 8: 274-278

Sengers, B.G.; Please, C.P.; Taylor, M.; Oreffo, R.O.C., 2009: Experimental-computational evaluation of human bone marrow stromal cell spreading on trabecular bone structures. The clinical application of macro-porous scaffolds for bone regeneration is significantly affected by the problem of insufficient cell colonization. Given the wide variety of different scaffold structures used for tissue engineering it is essentia...

Cholewicki, J.; Crisco, J.J.; Oxland, T.R.; Yamamoto, I.; Panjabi, M.M., 1996: Effects of posture and structure on three-dimensional coupled rotations in the lumbar spine. A biomechanical analysis. A biomechanical lumbar spine model was constructed to simulate three-dimensional spinal kinematics under the application of pure moments. Parametric analysis of the model allowed for the estimation of how much of the coupled motions could be predi...

Maramba-Ceballos, L., 1966: Philippines empirical dental remedies against pain. Dental Mirror 3(1): 13-16

Devine, J.B., 1956: John Berchmans Devine. Medical Journal of Australia 43(8): 340-341

Blondeau A.; Abdelmalik W.M.; Boukhary M., 1982: The nummulites of Thebes Formation, Nile Valley, Egypt. Revue de Micropaleontologie: , Pages 90-93. 1982.

Richette, P.; Brière, C.; Hoenen-Clavert, V.; Loeuille, D.; Bardin, T., 2007: Rasburicase for tophaceous gout not treatable with allopurinol: an exploratory study. Objective. To evaluate the short-term safety and outcome of 2 different experimental applications of rasburicase 0.2 mg/kg (monthly vs daily) in patients with tophaceous gout not treatable by allopurinol. Rasburicase could be useful for patients w...

Delgado M.A., 1990: Chromosome transfer from brewing to laboratory strains of yeast. Organizing Committee Of Biotec-90 From Genes to Bioproducts; 3rd Spanish Congress Of Biotechnology, Murcia, Spain, June Xxiv+409p Promociones Y Publicaciones Universitarias, S A : Barcelona, Spain; Dm: Murcia, Spain Illus Paper 101-106

Couteaux, M-M.; Mousseau, M.; Celerier, M-L.; Bottner, P., 1991: Increased atmospheric CO2 and litter quality: decomposition of sweet chestnut leaf litter with animal food webs of different complexities. Two-year-old chestnut [Castanea sativa] seedlings were potted (pot diameter 25 cm, height 50 cm) with top soil from a sweet chestnut forest and grown for two yr under ambient (350 p.p.m.) and enriched (700 p.p.m.) CO2 concentrations, in two natura...

Bhaskar, P.A., 1994: Effect of music on the human brain alpha rhythm. Journal of the Association of Physicians of India 42(4): 319-323