+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Production of mitogenic factors by cell types of bovine large estrogen-active and estrogen-inactive follicles

, : Production of mitogenic factors by cell types of bovine large estrogen-active and estrogen-inactive follicles. Journal of Animal Science 69(1): 237-245

This study investigated whether large follicles (estrogen-active and estrogen-inactive) of cows produce factors with mitogenic activity. Large, preovulatory follicles (greater than or equal to 9 mm in diameter) were classified as estrogen-active or -inactive based on ratio of estrogen: progesterone concentrations in follicular fluid. After incubation of granulosa cells and thecal tissues from follicles, granulosa cell conditioned media (GCM), thecal conditioned media (TCM) and follicular fluid (FFL) were evaluated for effects on proliferation of bovine aortic endothelial (BAE) and BALB/3T3 (3T3) cells. Pools of GCM, TCM and FFL stimulated proliferation of BAE and 3T3 in a dose-dependent fashion. Across all follicles (n = 20), GCM had greater stimulatory effect on proliferation of BAE than on proliferation of 3T3 (135 vs 115% of unconditioned media controls), whereas TCM stimulated proliferation of BAE and 3T3 to a similar extent (128 and 128%). Across type (GCM and TCM) of conditioned media, estrogen-active follicles stimulated proliferation of BAE more than proliferation of 3T3 (137 vs 121% of unconditioned media controls), whereas estrogen-inactive follicles stimulated proliferation of BAE and 3T3 to a similar extent (120 vs 122%). As observed for GCM, FFL across all follicles had a greater stimulatory effect on proliferation of BAE than on proliferation of 3T3 (159 vs 141%). Granulosa-conditioned media stimulated proliferation of BAE and 3T3 only when obtained from estrogen-active follicles; mitogenic activities of TCM and FFL were not influenced by type of follicle. These data demonstrate that granulosa cells of large preovulatory bovine follicles secrete a mitogenic factor(s) that is more stimulatory for proliferation of BAE than for 3T3.


PMID: 2005020

Other references

Foss G.V., 1938: Redke metally v zolotykh i sulfidnykh mestorozhdeniyakh Soyuza SSR. Rare metals in the gold and sulphide ores of Russia.

Kelley, R.B., 1948: Zebu-cross cattle in Northern Australia. Observations in Queensland. Quarter-bred Zebu-cross cows, milked once a day and kept on indifferent pasture, gave 20 per cent. more milk than British bred cows, due to their resistance to drought periods, and tolerance to ticks. The average daily yield of Jersey X Zebu cross...

Frantzen, S.; Maage, A.; Duinker, A.; Julshamn, K.; Iversen, S.A., 2015: A baseline study of metals in herring (Clupea harengus) from the Norwegian Sea, with focus on mercury, cadmium, arsenic and lead. The Norwegian spring spawning (NSS) herring is an ecologically and economically important fish population in the Norwegian Sea. It was the first of several Norwegian fish stocks subject to a baseline study designed to give a comprehensive account...

Jung, C.; Yee, S.; Kuhn, K., 1995: Electro-optic polymer light modulator based on surface plasmon resonance. The first experimental results, to our knowledge, for an optical modulator that makes use of an electro-optic polymer located between two thin silver films are presented. The methods used to fabricate the modulator are discussed. Initial results s...

Park, C.S.; Chung, W.G.; Kang, J.H.; Lee, K.H.; Roh, H.K.; Cha, Y.N., 1998: Determination of human FMO activity using caffeine metabolism and identification of FMO mutants. Clinical Pharmacology & Therapeutics 63(2): 207, Feb

Nuñez-Cumplido, E.; Perez-Calatayud, J.; Casares-Magaz, O.; Hernandez-Armas, J., 2016: Influence of source batch S dispersion on dosimetry for prostate cancer treatment with permanent implants. In clinical practice, specific air kerma strength (SK) value is used in treatment planning system (TPS) permanent brachytherapy implant calculations with (125)I and (103)Pd sources; in fact, commercial TPS provide only one SK input value for all i...

Lokot' L.I.; Gladkikh L.I., 1991: Correlation between body weight and linear dimensions in infusorians. The article proposes a new calculating method to define the individual mass of Infusoria. The method is based on the gradual dependence between mass and linear dimensions of the body. The parameters of equations for 18 predominant species and type...

van der Tas, G., 1966: Education and its relation to nursing. Sa Nursing Journal. Sa Verplegingstydskrif 33(2): 7-8 Contd

Rajan, P.; Hagman, A.; Sooriakumaran, P.; Nyberg, T.; Wallerstedt, A.; Adding, C.; Akre, O.; Carlsson, S.; Hosseini, A.; Olsson, M.; Egevad, L.; Wiklund, F.; Steineck, G.; Wiklund, N.Peter., 2017: Oncologic Outcomes After Robot-assisted Radical Prostatectomy: A Large European Single-centre Cohort with Median 10-Year Follow-up. Robot-assisted radical prostatectomy (RARP) for prostate cancer (PCa) treatment has been widely adopted with limited evidence for long-term (>5 yr) oncologic efficacy. To evaluate long-term oncologic outcomes following RARP. Prospective cohort...

Bankov, I.; Timanova, A.; Barrett, J., 1996: Methionine and cysteine metabolism in Fasciola hepatica. Radiolabel from the methyl groups of serine and methyltetrahydrofolate was readily incorporated into methionine in adult Fasciola hepatica, and a substantial proportion of the label from [35S]methionine appeared in cysteine. The data suggest that...