geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Prolactin secretion by mixed ACTH-prolactin pituitary adenoma cells in culture


, : Prolactin secretion by mixed ACTH-prolactin pituitary adenoma cells in culture. Acta Endocrinologica 108(4): 456-463

To characterize the functional aspect of prolactin (Prl) cells coexisting with corticotroph adenomas, pituitary adenoma cells obtained from a patient with Cushing's disease and a patient with Nelson's syndrome, who were associated with hyperprolactinaemia, were cultured in monolayer and their Prl responses to various secretagogues were compared with those of prolactinoma cells in culture. Immunohistochemistry performed in one of these two adenomas demonstrated the presence of Prl-containing cells in addition to ACTH cells. When ACTH-Prl adenoma cells were exposed to ovine corticotrophin-releasing factor (CRF), a dose-dependent increase in both ACTH and Prl secretion was observed, which was blocked by coincubation with hydrocortisone. In contrast, no stimulatory effect of CRF on Prl release was observed in all of the experiments using prolactinoma cells. Thyrotrophin-releasing hormone, which consistently stimulated Prl secretion in ACTH-Prl adenomas, was effective in triggering Prl release in only 25% of the prolactinomas. Exposure of the cultured cells to lysine vasopressin, growth hormone-releasing factor and vasoactive intestinal peptide resulted in an increase in ACTH and Prl secretion in one ACTH-Prl adenoma, however, none of the prolactinomas responded to these stimuli to secrete Prl. Dopamine and somatostatin, on the other hand, uniformly suppressed Prl secretion from ACTH-Prl adenomas as well as from prolactinoma cells. These results suggest that the mode of Prl secretion by mixed ACTH-Prl pituitary adenomas is not identical to that by pure prolactinomas and is, at least in part, common to that of ACTh secretion.

US$29.90

PMID: 2859725


Other references

Al-Khatib, M.; Shapiro, R.Jean.; Partovi, N.; Ting, L.S.L.; Levine, M.; Ensom, M.H.H., 2010: Limited sampling strategies for predicting area under the concentration-time curve of mycophenolic acid in islet transplant recipients. Mycophenolate mofetil is widely used in islet transplant recipients and its active metabolite, mycophenolic acid (MPA), exhibits wide pharmacokinetic variability. However, to our knowledge, no limited sampling strategy (LSS) exists for monitoring...

Moeller J.F.; Case J.F., 1990: Single unit analysis of visual interneurons in the mesopelagic mysid gnathophausia ingens. Society for Neuroscience Abstracts 16(1): 402

Bartek, J.K., 1994: The medication minute: Finasteride. Urologic Nursing 14(1): 29-30

Frouget, T., 2011: How to measure proteinuria: 24-hour urine collection versus protein/creatinine ratio in a random urine sample?. La Revue de Medecine Interne 31(12): 799-803

Afonso, M.B.; Rodrigues, Cília.M.P., 2017: microRevolution in understanding primary biliary cholangitis pathophysiology. Hepatology

Kako Y.; Higuchi S.; Hiroishi M.; Aoki T., 1984: Crystal structures of tropomyosin and its subunits and their variation through binding of troponin. The crystal structures of tropomyosin and its subunits (.alpha.- and .beta.-components) from porcine skeletal muscle were investigated. And at the same time, the structural variations through the binding of troponin with them were surveyed using t...

Zakerolhosseini, A.; Sokouti, M.; Pezeshkian, M., 2013: Home and Clinical Cardiovascular Care Center (H4C): a Framework for Integrating Body Sensor Networks and QTRU Cryptography System. Quick responds to heart attack patients before arriving to hospital is a very important factor. In this paper, a combined model of Body Sensor Network and Personal Digital Access using QTRU cipher algorithm in Wifi networks is presented to efficie...

Brodzinsky D.M., 1975: The role of conceptual tempo and stimulus characteristics in childrens humor development. Developmental Psychology 11(6): 843-850

Daoud, M.S.; Gibson, L.E.; Deremee, R.A.; Specks, U.; E.A.hary, R.A.; Su, W.P.D.niel, 1993: Cutaneous Wegeners granulomatosis Clinical, histopathologic, and immunologic features of 34 patients. Journal of Cutaneous Pathology 20(6): 537

Arul Pragasam, A.R., 1987: A three-point harness system for single-animal-drawn carts and implements. A harness system is presented which can be easily attached to any type of cart or implement, is within the economic means of the av. farmer, is easily accepted by the animal and gives better draught power.